Abstract. Introduction. The relevance of the research topic is due to the need to develop and scientifically substantiate physiological criteria for assessing working conditions in difficult cases according to indicators of the functional state of the body (in accordance with the section "General hygienic assessment of working conditions" of Guideline R 2.2.2006-05). The aim of the work is to determine changes in the functional state of the body in workers of mental, visual-intense, physical activity on the basis of complex physiological and hygienic studies to substantiate quantitative physiological criteria for different classes of labor intensity. Materials and methods. Complex industrial physiological and hygienic studies included an assessment of the degree of labor intensity with the analysis of retrospective data, physiological studies of the central nervous system, visual analyzer, neuromuscular apparatus, cardiovascular system of mental workers (52 groups), visually stressful (32), physical labor ( 71 professional group). Statistical processing of the obtained data was carried out using the statistical software Statistika 10, Microsoft Excel 2010. Research results. It is shown that the depth and time of the onset of physiological changes depend on the degree of labor intensity, which makes it possible to calculate the percentage of decrease in functions, taking into account the intensity class of the labor process. The list of physiological criteria for confirming the class of working conditions is determined by the characteristics of labor activity, with mental work these are indicators of concentration of attention and the volume of working memory, with visually intense labor, the critical frequency of fusion of light flashes, with physical labor, muscle endurance and heart rate. Conclusions. Quantitative physiological criteria have been established for various types of labor activity, which provide a valid evidence-based assessment of the class of working conditions according to the characteristics of the intensity of the labor process and control of the levels of the functional state.