Role of Pigment Epithelium-Derived Factor (PEDF) in Photoreceptor Cell Protection

2001 ◽  
pp. 119-126
Author(s):  
Wei Cao ◽  
Joyce Tombran-Tink ◽  
Rajesh Elias ◽  
Steven Sezate ◽  
James F. McGinnis
Keyword(s):  
Photoreceptor Cell ◽  
Pigment Epithelium ◽  
Cell Protection ◽  
Pigment Epithelium Derived Factor

scholarly journals A human model of Batten disease shows role of CLN3 in phagocytosis at the photoreceptor–RPE interface

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Cynthia Tang ◽  
Jimin Han ◽  
Sonal Dalvi ◽  
Kannan Manian ◽  
Lauren Winschel ◽  
...  
Keyword(s):  
Photoreceptor Cell ◽  
Vision Loss ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Cell Loss ◽  
Human Model ◽  
Lysosomal Storage ◽  
Rpe Cells ◽  
Cln3 Disease

AbstractMutations in CLN3 lead to photoreceptor cell loss in CLN3 disease, a lysosomal storage disorder characterized by childhood-onset vision loss, neurological impairment, and premature death. However, how CLN3 mutations cause photoreceptor cell death is not known. Here, we show that CLN3 is required for phagocytosis of photoreceptor outer segment (POS) by retinal pigment epithelium (RPE) cells, a cellular process essential for photoreceptor survival. Specifically, a proportion of CLN3 in human, mouse, and iPSC-RPE cells localized to RPE microvilli, the site of POS phagocytosis. Furthermore, patient-derived CLN3 disease iPSC-RPE cells showed decreased RPE microvilli density and reduced POS binding and ingestion. Notably, POS phagocytosis defect in CLN3 disease iPSC-RPE cells could be rescued by wild-type CLN3 gene supplementation. Altogether, these results illustrate a novel role of CLN3 in regulating POS phagocytosis and suggest a contribution of primary RPE dysfunction for photoreceptor cell loss in CLN3 disease that can be targeted by gene therapy.

Role of pigment epithelium-derived factor (PEDF) on arsenic-induced neuronal apoptosis

Chemosphere ◽  
2019 ◽  
Vol 215 ◽  
pp. 925-931 ◽  
Author(s):  
Wei Zhang ◽  
Xiaohui Cui ◽  
Yanhui Gao ◽  
Liyan Sun ◽  
Jing Wang ◽  
...  
Keyword(s):  
Neuronal Apoptosis ◽  
Pigment Epithelium ◽  
Pigment Epithelium Derived Factor

Protective role of pigment epithelium-derived factor (PEDF) in early phase of experimental diabetic retinopathy

2009 ◽  
Vol 25 (7) ◽  
pp. 678-686 ◽  
Author(s):  
Yumiko Yoshida ◽  
Sho-Ichi Yamagishi ◽  
Takanori Matsui ◽  
Yuko Jinnouchi ◽  
Kei Fukami ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Early Phase ◽  
Pigment Epithelium ◽  
Protective Role ◽  
Pigment Epithelium Derived Factor

Possible Role of Pigment-epithelium-derived Factor in Corneal Angiogenesis

2009 ◽  
Vol 03 (01) ◽  
pp. 64
Author(s):  
Oran Abdiu ◽  
Gysbert Van Setten ◽  
◽  
Keyword(s):  
Growth Factor ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Age Related Macular Degeneration ◽  
Antiangiogenic Agents ◽  
Special Role ◽  
Corneal Tissue ◽  
Pigment Epithelium Derived Factor ◽  
Corneal Angiogenesis

The detection of pigment-epithelium-derived factor (PEDF) in corneal tissue has allowed greater understanding of the avascularity of corneal tissue. The ability of the cornea to maintain the avascular nature of this tissue, also referred to as the angiogenic privilege of the cornea, could be partly attributed to the presence of this factor. This privilege is severely impaired by various diseases of the ocular surface associated with inflammation and infection that are often followed by neovascularisation, which compromises the transparency of the cornea and results in visual impairment. The rapidly increasing insights into the basic mechanisms controlling neovascularisation, i.e. balance of growth factor activation and enzymatic activity, has most recently led to the development of large-scale use of specific antiangiogenic agents in the treatment of neovascular age-related macular degeneration (AMD). Focusing on the effects of vascular endothelial growth factor (VEGF), the use of such agents, including bevacizumab (Avastin®), a humanised anti-VEGF monoclonal antibody originally used in the treatment of metastatic colorectal cancer, has been investigated in corneal angiogenesis. PEDF is only one of the many factors involved in ocular angiogenesis. However, although it is only a small protein, it has strong antiangiogenic actions that are expressed in the retinal pigment epithelial (RPE) layer, as well as in other parts of the eye. There are specific characteristics that could designate a special role for PEDF in the regulation of avascularity in the eye. In this article, we focus on corneal angiogenesis and highlight the special features of this somewhat unexplored cytokine, outlining the current knowledge and possible role of PEDF in corneal neovascularisation.

Augmented expression and secretion of adipose-derived pigment epithelium-derived factor does not alter local angiogenesis or contribute to the development of systemic metabolic derangements

2014 ◽  
Vol 306 (12) ◽  
pp. E1367-E1377 ◽  
Author(s):  
Thomas V. Lakeland ◽  
Melissa L. Borg ◽  
Maria Matzaris ◽  
Amany Abdelkader ◽  
Roger G. Evans ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Energy Expenditure ◽  
Body Mass ◽  
Pigment Epithelium ◽  
In Vivo Studies ◽  
Whole Body ◽  
Pigment Epithelium Derived Factor ◽  
Metabolic Role

Impaired coupling of adipose tissue expansion and vascularization is proposed to lead to adipocyte hypoxia and inflammation, which in turn contributes to systemic metabolic derangements. Pigment epithelium-derived factor (PEDF) is a powerful antiangiogenic factor that is secreted by adipocytes, elevated in obesity, and implicated in the development of insulin resistance. We explored the angiogenic and metabolic role of adipose-derived PEDF through in vivo studies of mice with overexpression of PEDF in adipocytes (PEDF-aP2). PEDF expression in white adipocytes and PEDF secretion from adipose tissue was increased in transgenic mice, but circulating levels of PEDF were not increased. Overexpression of PEDF did not alter vascularization, the partial pressure of O2, cellular hypoxia, or gene expression of inflammatory markers in adipose tissue. Energy expenditure and metabolic substrate utilization, body mass, and adiposity were not altered in PEDF-aP2 mice. Whole body glycemic control was normal as assessed by glucose and insulin tolerance tests, and adipocyte-specific glucose uptake was unaffected by PEDF overexpression. Adipocyte lipolysis was increased in PEDF-aP2 mice and associated with increased adipose triglyceride lipase and decreased perilipin 1 expression. Experiments conducted in mice rendered obese by high-fat feeding showed no differences between PEDF-aP2 and wild-type mice for body mass, adiposity, whole body energy expenditure, glucose tolerance, or adipose tissue oxygenation. Together, these data indicate that adipocyte-generated PEDF enhances lipolysis but question the role of PEDF as a major antiangiogenic or proinflammatory mediator in adipose tissue in vivo.

The role of pigment epithelium-derived factor in protecting against cellular stress

2019 ◽  
Vol 53 (11-12) ◽  
pp. 1166-1180 ◽  
Author(s):  
Naomi Brook ◽  
Emily Brook ◽  
Arun Dharmarajan ◽  
Arlene Chan ◽  
Crispin R. Dass
Keyword(s):  
Pigment Epithelium ◽  
Cellular Stress ◽  
Pigment Epithelium Derived Factor

scholarly journals Pigment Epithelium-Derived Factor (PEDF) Fragments Prevent Mouse Cone Photoreceptor Cell Loss Induced by Focal Phototoxicity In Vivo

2020 ◽  
Vol 21 (19) ◽  
pp. 7242
Author(s):  
Francisco J. Valiente-Soriano ◽  
Johnny Di Pierdomenico ◽  
Diego García-Ayuso ◽  
Arturo Ortín-Martínez ◽  
Juan A. Miralles de Imperial-Ollero ◽  
...  
Keyword(s):  
Photoreceptor Cell ◽  
Cell Damage ◽  
Pigment Epithelium ◽  
Cell Loss ◽  
Negative Control ◽  
Cone Photoreceptor ◽  
Peptide Fragments ◽  
Neurotrophic Activity ◽  
Pigment Epithelium Derived Factor

Here, we evaluated the effects of PEDF (pigment epithelium-derived factor) and PEDF peptides on cone-photoreceptor cell damage in a mouse model of focal LED-induced phototoxicity (LIP) in vivo. Swiss mice were dark-adapted overnight, anesthetized, and their left eyes were exposed to a blue LED placed over the cornea. Immediately after, intravitreal injection of PEDF, PEDF-peptide fragments 17-mer, 17-mer[H105A] or 17-mer[R99A] (all at 10 pmol) were administered into the left eye of each animal. BDNF (92 pmol) and bFGF (27 pmol) injections were positive controls, and vehicle negative control. After 7 days, LIP resulted in a consistent circular lesion located in the supratemporal quadrant and the number of S-cones were counted within an area centered on the lesion. Retinas treated with effectors had significantly greater S-cone numbers (PEDF (60%), 17-mer (56%), 17-mer [H105A] (57%), BDNF (64%) or bFGF (60%)) relative to their corresponding vehicle groups (≈42%). The 17-mer[R99A] with no PEDF receptor binding and no neurotrophic activity, PEDF combined with a molar excess of the PEDF receptor blocker P1 peptide, or with a PEDF-R enzymatic inhibitor had undetectable effects in S-cone survival. The findings demonstrated that the cone survival effects were mediated via interactions between the 17-mer region of the PEDF molecule and its PEDF-R receptor.

scholarly journals The Role of Pigment Epithelium-Derived Factor in the Pathophysiology and Treatment of Ovarian Hyperstimulation Syndrome in Mice

2013 ◽  
Vol 98 (2) ◽  
pp. E258-E266 ◽  
Author(s):  
Dana Chuderland ◽  
Ido Ben-Ami ◽  
Ruth Kaplan-Kraicer ◽  
Hadas Grossman ◽  
Raphael Ron-El ◽  
...  
Keyword(s):  
Ovarian Hyperstimulation Syndrome ◽  
Pigment Epithelium ◽  
Ovarian Hyperstimulation ◽  
Pigment Epithelium Derived Factor

Pathophysiological Role of Pigment Epithelium-Derived Factor (PEDF) in Hepatic Disorders

2010 ◽  
Vol 17 (19) ◽  
pp. 1995-2000 ◽  
Author(s):  
S.-I. Yamagishi ◽  
T. Matsui ◽  
T. Kawaguchi ◽  
M. Sata
Keyword(s):  
Pigment Epithelium ◽  
Pigment Epithelium Derived Factor ◽  
Pathophysiological Role
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