Suppression of Human Melanoma Metastasis in SCID Mice with Antibodies to the EGF-Receptor

1994 ◽  
pp. 119-125
Author(s):  
B. M. Mueller ◽  
R. A. Reisfeld
Keyword(s):  
Egf Receptor ◽  
Human Melanoma ◽  
Scid Mice ◽  
Melanoma Metastasis

scholarly journals Deuterium Oxide (D2O) Induces Early Stress Response Gene Expression and Impairs Growth and Metastasis of Experimental Malignant Melanoma

Cancers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 605
Author(s):  
Jana Jandova ◽  
Anh B. Hua ◽  
Jocelyn Fimbres ◽  
Georg T. Wondrak
Keyword(s):  
Gene Expression ◽  
Stress Response ◽  
Malignant Melanoma ◽  
Tumor Growth ◽  
Deuterium Oxide ◽  
Human Melanoma ◽  
Pharmacological Intervention ◽  
Melanoma Metastasis ◽  
Response Gene ◽  
Stress Response Gene

There are two stable isotopes of hydrogen, protium (1H) and deuterium (2H; D). Cellular stress response dysregulation in cancer represents both a major pathological driving force and a promising therapeutic target, but the molecular consequences and potential therapeutic impact of deuterium (2H)-stress on cancer cells remain largely unexplored. We have examined the anti-proliferative and apoptogenic effects of deuterium oxide (D2O; ‘heavy water’) together with stress response gene expression profiling in panels of malignant melanoma (A375V600E, A375NRAS, G361, LOX-IMVI), and pancreatic ductal adenocarcinoma (PANC-1, Capan-2, or MIA PaCa-2) cells with inclusion of human diploid Hs27 skin fibroblasts. Moreover, we have examined the efficacy of D2O-based pharmacological intervention in murine models of human melanoma tumor growth and metastasis. D2O-induction of apoptosis was substantiated by AV-PI flow cytometry, immunodetection of PARP-1, and pro-caspase 3 cleavage, and rescue by pan-caspase inhibition. Differential array analysis revealed early modulation of stress response gene expression in both A375 melanoma and PANC-1 adenocarcinoma cells elicited by D2O (90%; ≤6 h) (upregulated: CDKN1A, DDIT3, EGR1, GADD45A, HMOX1, NFKBIA, or SOD2 (up to 9-fold; p < 0.01)) confirmed by independent RT-qPCR analysis. Immunoblot analysis revealed rapid onset of D2O-induced stress response phospho-protein activation (p-ERK, p-JNK, p-eIF2α, or p-H2AX) or attenuation (p-AKT). Feasibility of D2O-based chemotherapeutic intervention (drinking water (30% w/w)) was demonstrated in a severe combined immunodeficiency (SCID) mouse melanoma metastasis model using luciferase-expressing A375-Luc2 cells. Lung tumor burden (visualized by bioluminescence imaging) was attenuated by D2O, and inhibition of invasiveness was also confirmed in an in vitro Matrigel transwell invasion assay. D2O supplementation also suppressed tumor growth in a murine xenograft model of human melanoma, and median survival was significantly increased without causing adverse effects. These data demonstrate for the first time that systemic D2O administration impairs growth and metastasis of malignant melanoma through the pharmacological induction of deuterium (2H)-stress.

Sex-dependent liver colonization of human melanoma in SCID mice—role of host defense mechanisms

2012 ◽  
Vol 30 (4) ◽  
pp. 497-506 ◽  
Author(s):  
Judit Dobos ◽  
Anita Mohos ◽  
József Tóvári ◽  
Erzsébet Rásó ◽  
Tamás Lőrincz ◽  
...  
Keyword(s):  
Host Defense ◽  
Defense Mechanisms ◽  
Human Melanoma ◽  
Scid Mice

scholarly journals Human Melanoma-Cell Metabolic Profiling: Identification of Novel Biomarkers Indicating Metastasis

2020 ◽  
Vol 21 (7) ◽  
pp. 2436 ◽  
Author(s):  
Mariangela Kosmopoulou ◽  
Aikaterini F. Giannopoulou ◽  
Aikaterini Iliou ◽  
Dimitra Benaki ◽  
Aristeidis Panagiotakis ◽  
...  
Keyword(s):  
Discriminant Analysis ◽  
High Resolution ◽  
Least Squares ◽  
Partial Least Squares ◽  
Cell Lines ◽  
High Performance ◽  
Principal Component ◽  
Metastatic Tumor ◽  
Human Melanoma ◽  
Melanoma Metastasis

Melanoma is the most aggressive type of skin cancer, leading to metabolic rewiring and enhancement of metastatic transformation. Efforts to improve its early and accurate diagnosis are largely based on preclinical models and especially cell lines. Hence, we herein present a combinational Nuclear Magnetic Resonance (NMR)- and Ultra High Performance Liquid Chromatography-High-Resolution Tandem Mass Spectrometry (UHPLC-HRMS/MS)-mediated untargeted metabolomic profiling of melanoma cells, to landscape metabolic alterations likely controlling metastasis. The cell lines WM115 and WM2664, which belong to the same patient, were examined, with WM115 being derived from a primary, pre-metastatic, tumor and WM2664 clonally expanded from lymph-node metastases. Metabolite samples were analyzed using NMR and UHPLC-HRMS. Multivariate statistical analysis of high resolution NMR and MS (positive and negative ionization) results was performed by Principal Component Analysis (PCA), Partial Least Squares-Discriminant Analysis (PLS-DA) and Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA), while metastasis-related biomarkers were determined on the basis of VIP lists, S-plots and Student’s t-tests. Receiver Operating Characteristic (ROC) curves of NMR and MS data revealed significantly differentiated metabolite profiles for each cell line, with WM115 being mainly characterized by upregulated levels of phosphocholine, choline, guanosine and inosine. Interestingly, WM2664 showed notably increased contents of hypoxanthine, myo-inositol, glutamic acid, organic acids, purines, pyrimidines, AMP, ADP, ATP and UDP(s), thus indicating the critical roles of purine, pyrimidine and amino acid metabolism during human melanoma metastasis.

scholarly journals Plasma Treatment Limits Human Melanoma Spheroid Growth and Metastasis Independent of the Ambient Gas Composition

Cancers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2570 ◽  
Author(s):  
Sybille Hasse ◽  
Tita Meder ◽  
Eric Freund ◽  
Thomas von Woedtke ◽  
Sander Bekeschus
Keyword(s):  
Plasma Treatment ◽  
Treatment Time ◽  
Argon Plasma ◽  
Ambient Air ◽  
Human Melanoma ◽  
Melanoma Metastasis ◽  
Spheroid Growth ◽  
Tumor Entity ◽  
Melanoma Treatment ◽  
Jet Plasma

Melanoma skin cancer is still a deadly disease despite recent advances in therapy. Previous studies have suggested medical plasma technology as a promising modality for melanoma treatment. However, the efficacy of plasmas operated under different ambient air conditions and the comparison of direct and indirect plasma treatments are mostly unexplored for this tumor entity. Moreover, exactly how plasma treatment affects melanoma metastasis has still not been explained. Using 3D tumor spheroid models and high-content imaging technology, we addressed these questions by utilizing one metastatic and one non-metastatic human melanoma cell line targeted with an argon plasma jet. Plasma treatment was toxic in both cell lines. Modulating the oxygen and nitrogen ambient air composition (100/0, 75/25, 50/50, 25/75, and 0/100) gave similar toxicity and reduced the spheroid growth for all conditions. This was the case for both direct and indirect treatments, with the former showing a treatment time-dependent response while the latter resulted in cytotoxicity with the longest treatment time investigated. Live-cell imaging of in-gel cultured spheroids indicated that plasma treatment did not enhance metastasis, and flow cytometry showed a significant modulation of S100A4 but not in any of the five other metastasis-related markers (β-catenin, E-cadherin, LEF1, SLUG, and ZEB1) investigated.

Suppression of Human Melanoma Metastasis by the Metastasis Suppressor Gene, BRMS1

2002 ◽  
Vol 273 (2) ◽  
pp. 229-239 ◽  
Author(s):  
Lalita A Shevde ◽  
Rajeev S Samant ◽  
Steven F Goldberg ◽  
Tabo Sikaneta ◽  
Alessandro Alessandrini ◽  
...  
Keyword(s):  
Suppressor Gene ◽  
Human Melanoma ◽  
Metastasis Suppressor ◽  
Melanoma Metastasis ◽  
Metastasis Suppressor Gene

scholarly journals Differential patterns ofHOX gene expression are associated with specific integrin and ICAM profiles in clonal populations isolated from a single human melanoma metastasis

1996 ◽  
Vol 66 (5) ◽  
pp. 692-697 ◽  
Author(s):  
Clemente Cillo ◽  
Monica Cantile ◽  
Roberta Mortarini ◽  
Pasquale Barba ◽  
Giorgio Parmiani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Melanoma ◽  
Melanoma Metastasis

Abstract 3430: Role of Tip60 in human melanoma cell migration, melanoma metastasis and patient survival

2012 ◽  
Author(s):  
Guangdi Chen ◽  
Yabin Cheng ◽  
Zhizhong Zhang ◽  
Magdalena Martinka ◽  
Gang Li
Keyword(s):  
Cell Migration ◽  
Melanoma Cell ◽  
Patient Survival ◽  
Human Melanoma ◽  
Human Melanoma Cell ◽  
Melanoma Metastasis

Cimetidine and a tamoxifen derivate reduce tumour formation in SCID mice xenotransplanted with a human melanoma cell line

2002 ◽  
Vol 12 (3) ◽  
pp. 231-240 ◽  
Author(s):  
N. Szincsák ◽  
H. Hegyesi ◽  
J. Hunyadi ◽  
G. Martin ◽  
E. Lázár-Molnár ◽  
...  
Keyword(s):  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Human Melanoma ◽  
Scid Mice ◽  
Human Melanoma Cell Line ◽  
Human Melanoma Cell ◽  
Tumour Formation

C-myc gene expression positively correlates with metastatic potential of human melanoma in SCID mice

1997 ◽  
Vol 7 (Supplement 1) ◽  
pp. S6 ◽  
Author(s):  
H Schlagbauer-Wadl ◽  
M Griffioen ◽  
Elsas A van ◽  
P I Schrier ◽  
T Pustelnik ◽  
...  
Keyword(s):  
Gene Expression ◽  
Metastatic Potential ◽  
Human Melanoma ◽  
Scid Mice ◽  
Myc Gene

scholarly journals Antibody Directed against Human YKL-40 Increases Tumor Volume in a Human Melanoma Xenograft Model in Scid Mice

PLoS ONE ◽  
2014 ◽  
Vol 9 (4) ◽  
pp. e95822 ◽  
Author(s):  
Johannes Salamon ◽  
Tatjana Hoffmann ◽  
Eva Elies ◽  
Kersten Peldschus ◽  
Julia S. Johansen ◽  
...  
Keyword(s):  
Tumor Volume ◽  
Xenograft Model ◽  
Human Melanoma ◽  
Scid Mice ◽  
Human Melanoma Xenograft ◽  
Melanoma Xenograft
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