Do the Two Types of IL-8 Receptors on Human Neutrophils Mediate Different Cellular Responses?

1993 ◽  
pp. 207-207 ◽  
Author(s):  
C. Lam ◽  
A. Tuschil ◽  
I. J. D. Lindley
Keyword(s):  
Cellular Responses ◽  
Human Neutrophils

Receptor Binding Kinetics and Cellular Responses of Six N-Formyl Peptide Agonists in Human Neutrophils†

Biochemistry ◽  
2004 ◽  
Vol 43 (25) ◽  
pp. 8204-8216 ◽  
Author(s):  
Anna Waller ◽  
Karyn L. Sutton ◽  
Tamara L. Kinzer-Ursem ◽  
Afaf Absood ◽  
John R. Traynor ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Cellular Responses ◽  
Binding Kinetics ◽  
Human Neutrophils ◽  
Formyl Peptide

Comparison of 1-oleoyl-2-acetyl-glycerol and phorbol myristate acetate as secretagogues for human neutrophils

1986 ◽  
Vol 64 (8) ◽  
pp. 1149-1152 ◽  
Author(s):  
Kenneth Wong ◽  
Christine Chew
Keyword(s):  
Phorbol Myristate Acetate ◽  
Cellular Responses ◽  
Current Evidence ◽  
Human Neutrophils ◽  
Myristate Acetate ◽  
First Order ◽  
Regulatory Constraints ◽  
Kinase C ◽  
Rate Of Decay ◽  
The Stability

Cellular responses induced in human neutrophils by the synthetic diacylglycerol, 1-oleoyl-2-acetyl-glycerol (OAG), paralleled those induced by phorbol myristate acetate (PMA). Like PMA, OAG caused the preferential release of enzymes from specific granules and promoted superoxide (O2−) generation. The efficacy of OAG was similar to that for PMA, but its potency was lower by four orders of magnitude. First derivative kinetic analysis showed that rates of O2− generation elicited by PMA decayed exponentially in a first order manner; the half life was found to be 21 ± 6 min. Results obtained in studies carried out with high OAG concentrations were similar except that after 40 min, the rate of decay increased and became complex order. This difference was attributed to the greater susceptibility of OAG to metabolic alteration, and was reflected in the NADPH oxidase activity of granule rich membrane fractions (GRF) of cells stimulated for 90 min with PMA or OAG. It was found that the O2− generating activity of the PMA treated GRF was significantly greater than that for the OAG treated fraction. Current evidence indicates that cellular responses arise from direct activation of protein kinase C by PMA-OAG. The stability of this complex and the bypassing of normal regulatory constraints may account for the relative longevity of the PMA–OAG O2− respiratory burst.

scholarly journals CD16b associates with high-density, detergent-resistant membranes in human neutrophils

2005 ◽  
Vol 393 (1) ◽  
pp. 351-359 ◽  
Author(s):  
Maria J. G. Fernandes ◽  
Emmanuelle Rollet-Labelle ◽  
Guillaume Paré ◽  
Sébastien Marois ◽  
Marie-Lisane Tremblay ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Plasma Membrane ◽  
Direct Evidence ◽  
Cellular Responses ◽  
High Density ◽  
Polymorphonuclear Neutrophils ◽  
Human Neutrophils ◽  
Glycosylphosphatidylinositol Anchor ◽  
Detergent Resistant Membranes ◽  
Receptor Ligation

CD16b is unique in that it is the only Fc receptor linked to the plasma membrane by a GPI (glycosylphosphatidylinositol) anchor. GPI-anchored proteins often preferentially localize to DRMs (detergent-resistant membranes) that are rich in sphingolipids and cholesterol and play an important role in signal transduction. Even though the responses to CD16b engagement have been intensively investigated, the importance of DRM integrity for CD16b signalling has not been characterized in human neutrophils. We provide direct evidence that CD16b constitutively partitions with both low- and high-density DRMs. Moreover, upon CD16b engagement, a significant increase in the amount of the receptor is observed in high-density DRMs. Similarly to CD16b, CD11b also resides in low- and high-density DRMs. In contrast with CD16b, the partitioning of CD11b in DRMs does not change in response to CD16b engagement. We also provide evidence for the implication of Syk in CD16b signalling and its partitioning to DRMs in resting and activated PMNs (polymorphonuclear neutrophils). Additionally, DRM-disrupting agents, such as nystatin and methyl-β-cyclodextrin, alter cellular responses to CD16b receptor ligation. Notably, a significant increase in the mobilization of intracellular Ca2+ and in tyrosine phosphorylation of intracellular substrates after CD16b engagement is observed. Altogether, the results of this study provide evidence that high-density DRMs play a role in CD16b signalling in human neutrophils.

Neutrophil Myeloperoxidase Destruction by Ultraviolet Irradiation

1988 ◽  
Vol 46 ◽  
pp. 138-139
Author(s):  
J. Hanker ◽  
B. Giammara ◽  
G. Strauss
Keyword(s):  
Uv Radiation ◽  
Myeloid Leukemia ◽  
Stratospheric Ozone ◽  
Psoriasis Patient ◽  
Whole Body ◽  
Human Neutrophils ◽  
Cupric Nitrate ◽  
The Earth ◽  
Routine Handling ◽  
Uvb Phototherapy

Only a fraction of the UV radiation emitted by the sun reaches the earth; most of the UVB (290-320nm) is eliminated by stratospheric ozone. There is increasing concern, however, that man-made chemicals are damaging this ozone layer. Although the effects of UV on DNA or as a carcinogen are widely known, preleukemia and acute myeloid leukemia (AML) have only rarely been reported in psoriasis patients treated with 8-methoxypsoralen and UV (PUVA). It was therefore of interest to study the effects of UV on the myeloperoxidase (MP) activity of human neutrophils. The peroxidase activity of enriched leukocyte preparations on coverslips was shown cytochemically with a diaminobenzidine medium and cupric nitrate intensification.Control samples (Figs. 1,4,5) of human bloods that were not specifically exposed to UV radiation or light except during routine handling were compared with samples which had been exposed in one of several different ways. One preparation (Fig. 2) was from a psoriasis patient who had received whole-body UVB phototherapy repeatedly.

The Response of Testis Cells to Low Dose X-Irradiation

1981 ◽  
Vol 39 ◽  
pp. 542-543
Author(s):  
D. E. Philpott ◽  
W. Sapp ◽  
C. Williams ◽  
Joann Stevenson ◽  
S. Black
Keyword(s):  
Electron Microscopy ◽  
Stem Cell ◽  
Light Microscopy ◽  
Dose Level ◽  
Cross Sections ◽  
Low Dose ◽  
Light And Electron Microscopy ◽  
Cellular Responses ◽  
Post Irradiation ◽  
X Irradiation

The response of spermatogonial cells to X-irradiation is well documented. It has been shown that there is a radiation resistent stem cell (As) which, after irradiation, replenishes the seminiferous epithelium. Most investigations in this area have dealt with radiation dosages of 100R or more. This study was undertaken to observe cellular responses at doses less than 100R of X-irradiation utilizing a system in which the tissue can be used for light and electron microscopy.Brown B6D2F1 mice aged 16 weeks were exposed to X-irradiation (225KeV; 15mA; filter 0.35 Cu; 50-60 R/min). Four mice were irradiated at each dose level between 1 and 100 rads. Testes were removed 3 days post-irradiation, fixed, and embedded. Sections were cut at 2 microns for light microscopy. After staining, surviving spermatogonia were identified and counted in tubule cross sections. The surviving fraction of spermatogonia compared to control, S/S0, was plotted against dose to give the curve shown in Fig. 1.

Application of high-resolution field-emission LVSEM, backscatter imaging, immunogold staining to definition of the spatial distributions of two human neutrophil adherence receptors

1993 ◽  
Vol 51 ◽  
pp. 36-37
Author(s):  
Robert D. Nelson ◽  
Sharon R. Hasslen ◽  
Stanley L. Erlandsen
Keyword(s):  
Cell Surface ◽  
Surface Membrane ◽  
Three Dimensional ◽  
Human Neutrophils ◽  
Spatial Distributions ◽  
Immunogold Staining ◽  
Functional Control ◽  
Neutrophil Adherence ◽  
Definition Of ◽  
Mode Of Attachment

Receptors are commonly defined in terms of number per cell, affinity for ligand, chemical structure, mode of attachment to the cell surface, and mechanism of signal transduction. We propose to show that knowledge of spatial distribution of receptors on the cell surface can provide additional clues to their function and components of functional control.L-selectin and Mac-1 denote two receptor populations on the neutrophil surface that mediate neutrophil-endothelial cell adherence interactions and provide for targeting of neutrophil recruitment to sites of inflammation. We have studied the spatial distributions of these receptors using LVSEM and backscatter imaging of isolated human neutrophils stained with mouse anti-receptor (primary) antibody and goat anti-mouse (secondary) antibody conjugated to 12 nm colloidal gold. This combination of techniques provides for three-dimensional analysis of the expression of these receptors on different surface membrane domains of the neutrophil: the ruffles and microvilli that project from the cell surface, and the cell body between these projecting structures.

Inhibition of Apoptosis in Human Neutrophils by Helicobacter pylori Water-Soluble Surface Proteins

2001 ◽  
Vol 36 (6) ◽  
pp. 589-600 ◽  
Author(s):  
J. S. Kim ◽  
J. M. Kim ◽  
H. C. Jung ◽  
I. S. Song ◽  
C. Y. Kim
Keyword(s):  
Helicobacter Pylori ◽  
Water Soluble ◽  
Surface Proteins ◽  
Human Neutrophils

Author response for "T cell‐derived cytokines enhance the antigen‐presenting capacity of human neutrophils"

2019 ◽  
Author(s):  
Nazanin Samadi ◽  
Dominika Polak ◽  
Claudia Kitzmüller ◽  
Peter Steinberger ◽  
Gerhard J. Zlabinger ◽  
...  
Keyword(s):  
T Cell ◽  
Author Response ◽  
Human Neutrophils ◽  
Antigen Presenting
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