Evaluation of Quenching and Extraction Methods for Nucleotide/Nucleotide Sugar Analysis

2015 ◽  
pp. 361-372 ◽  
Author(s):  
Katrin Braasch ◽  
Carina Villacrés ◽  
Michael Butler
Keyword(s):  
Extraction Methods ◽  
Nucleotide Sugar ◽  
Sugar Analysis

scholarly journals Nucleotide and Nucleotide Sugar Analysis in Cell Extracts by Capillary Electrophoresis

2016 ◽  
Vol 70 (10) ◽  
pp. 732-735 ◽  
Author(s):  
Blanka Bucsella ◽  
Antoine Fornage ◽  
Catherine Le Denmat ◽  
Franka Kálmán
Keyword(s):  
Capillary Electrophoresis ◽  
Nucleotide Sugar ◽  
Sugar Analysis ◽  
Cell Extracts

scholarly journals Cellular Fucosylation Inhibitors Based on Fluorinated Fucose-1-Phosphates

2020 ◽  
Author(s):  
Johan Pijnenborg ◽  
eline visser ◽  
Marek Noga ◽  
Emiel Rossing ◽  
Raisa Veizaj ◽  
...  
Keyword(s):  
Nucleotide Sugar ◽  
Sugar Analysis ◽  
Health And Disease ◽  
Cancer Inflammation ◽  
Therapeutic Avenue

<a>Fucosylation of glycans impacts a myriad of physiological and pathological processes. Inhibition of fucose expression emerges as a potential therapeutic avenue for example in cancer, inflammation, and infection. In this study, we found that protected 2-fluorofucose 1-phosphate efficiently inhibits cellular fucosylation with a four to seven times higher potency than known inhibitor 2FF, independently of the anomeric stereochemistry. Nucleotide sugar analysis revealed that both the α- and β-GDP-2FF anomers are formed inside the cell. In conclusion, we developed A2FF1P and B2FF1P as potent new tools for studying the role of fucosylation in health and disease</a> and they are potential therapeutic candidates.

scholarly journals Cellular Fucosylation Inhibitors Based on Fluorinated Fucose-1-Phosphates

2020 ◽  
Author(s):  
Johan Pijnenborg ◽  
eline visser ◽  
Marek Noga ◽  
Emiel Rossing ◽  
Raisa Veizaj ◽  
...  
Keyword(s):  
Nucleotide Sugar ◽  
Sugar Analysis ◽  
Health And Disease ◽  
Cancer Inflammation ◽  
Therapeutic Avenue

<a>Fucosylation of glycans impacts a myriad of physiological and pathological processes. Inhibition of fucose expression emerges as a potential therapeutic avenue for example in cancer, inflammation, and infection. In this study, we found that protected 2-fluorofucose 1-phosphate efficiently inhibits cellular fucosylation with a four to seven times higher potency than known inhibitor 2FF, independently of the anomeric stereochemistry. Nucleotide sugar analysis revealed that both the α- and β-GDP-2FF anomers are formed inside the cell. In conclusion, we developed A2FF1P and B2FF1P as potent new tools for studying the role of fucosylation in health and disease</a> and they are potential therapeutic candidates.

The effect of different extraction methods on chemical composition profile and pharmacological activities of essential oils and hydrosols

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
F Ghavidel ◽  
MM Zarshenas ◽  
A Sakhteman ◽  
A Gholami ◽  
Y Ghasemi ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Extraction Methods ◽  
Composition Profile ◽  
Pharmacological Activities

Characterization of dissolved organic matter (DOM) extracted from soils by hot water percolation (HWP)

2010 ◽  
Vol 59 (1) ◽  
pp. 99-108 ◽  
Author(s):  
M. Takács ◽  
Gy. Füleky
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Natural Organic Matter ◽  
Extraction Methods ◽  
Soil Samples ◽  
Hot Water ◽  
Soil Extracts ◽  
Water Percolation ◽  
Soil Humic Acid

The Hot Water Percolation (HWP) technique for preparing soil extracts has several advantages: it is easily carried out, fast, and several parameters can be measured from the same solution. The object of this study was to examine the possible use of HWP extracts for the characterization of soil organic matter. The HPLC-SEC chromatograms, UV-VIS and fluorescence properties of the HWP extracts were studied and the results were compared with those of the International Humic Substances Society (IHSS) Soil Humic Acid (HA), IHSS Soil Fulvic Acid (FA) and IHSS Suwannee Natural Organic Matter (NOM) standards as well as their HA counterparts isolated by traditional extraction methods from the original soil samples. The DOM of the HWP solution is probably a mixture of organic materials, which have some characteristics similar to the Soil FA fractions and NOM. The HWP extracted organic material can be studied and characterized using simple techniques, like UV-VIS and fluorescence spectroscopy.

New Feature Vectors using GFCC for Speaker Identification

2018 ◽  
Vol 6 (8) ◽  
pp. 243
Author(s):  
A. Nagesh
Keyword(s):  
Speaker Recognition ◽  
Speaker Identification ◽  
Signal To Noise Ratio ◽  
Main Idea ◽  
Extraction Methods ◽  
Identification System ◽  
Identification Performance ◽  
Feature Vectors ◽  
Overall Performance ◽  
New Feature

The feature vectors of speaker identification system plays a crucial role in the overall performance of the system. There are many new feature vectors extraction methods based on MFCC, but ultimately we want to maximize the performance of SID system.  The objective of this paper to derive Gammatone Frequency Cepstral Coefficients (GFCC) based a new set of feature vectors using Gaussian Mixer model (GMM) for speaker identification. The MFCC are the default feature vectors for speaker recognition, but they are not very robust at the presence of additive noise. The GFCC features in recent studies have shown very good robustness against noise and acoustic change. The main idea is  GFCC features based on GMM feature extraction is to improve the overall speaker identification performance in low signal to noise ratio (SNR) conditions.

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