scholarly journals Measuring Gli2 Phosphorylation by Selected Reaction Monitoring Mass Spectrometry

2015 ◽  
pp. 105-123
Author(s):  
Robert Ahrends ◽  
Pawel Niewiadomski ◽  
Mary N. Teruel ◽  
Rajat Rohatgi
Keyword(s):  
Mass Spectrometry ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring

scholarly journals Selected reaction monitoring mass spectrometry of mastitis milk reveals pathogen-specific regulation of bovine host response proteins

2018 ◽  
Vol 101 (7) ◽  
pp. 6532-6541 ◽  
Author(s):  
Ulrike Kusebauch ◽  
Lorenzo E. Hernández-Castellano ◽  
Stine L. Bislev ◽  
Robert L. Moritz ◽  
Christine M. Røntved ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Host Response ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
Specific Regulation

scholarly journals IsoSearch: An Untargeted and Unbiased Metabolite and Lipid Isotopomer Tracing Strategy from HR-LC-MS/MS Datasets

2020 ◽  
Vol 3 (3) ◽  
pp. 54
Author(s):  
He Huang ◽  
Min Yuan ◽  
Phillip Seitzer ◽  
Susan Ludwigsen ◽  
John M. Asara
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Defense Mechanism ◽  
High Resolution Mass Spectrometry ◽  
Pentose Phosphate ◽  
Selected Reaction Monitoring ◽  
Reaction Monitoring ◽  
Triglyceride Accumulation ◽  
Pathway Inhibition ◽  
Mcf 7

Stable isotopic tracer analysis is a technique used to determine carbon or nitrogen atom incorporation into biological systems. A number of mass spectrometry based approaches have been developed for this purpose, including high-resolution tandem mass spectrometry (HR-LC-MS/MS), selected reaction monitoring (SRM) and parallel reaction monitoring (PRM). We have developed an approach for analyzing untargeted metabolomic and lipidomic datasets using high-resolution mass spectrometry with polarity switching and implemented our approach in the open-source R script IsoSearch and in Scaffold Elements software. Using our strategy, which requires an unlabeled reference dataset and isotope labeled datasets across various biological conditions, we traced metabolic isotopomer alterations in breast cancer cells (MCF-7) treated with the metabolic drugs 2-deoxy-glucose, 6-aminonicotinamide, compound 968, and rapamycin. Metabolites and lipids were first identified by the commercial software Scaffold Elements and LipidSearch, then IsoSearch successfully profiled the 13C-isotopomers extracted metabolites and lipids from 13C-glucose labeled MCF-7 cells. The results interpreted known models, such as glycolysis and pentose phosphate pathway inhibition, but also helped to discover new metabolic/lipid flux patterns, including a reactive oxygen species (ROS) defense mechanism induced by 6AN and triglyceride accumulation in rapamycin treated cells. The results suggest the IsoSearch/Scaffold Elements platform is effective for studying metabolic tracer analysis in diseases, drug metabolism, and metabolic engineering for both polar metabolites and non-polar lipids.

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