Lentivirus-Based Stable Gene Delivery into Intestinal Organoids

2016 ◽  
pp. 13-21 ◽  
Author(s):  
Yoshiaki Maru ◽  
Kaoru Orihashi ◽  
Yoshitaka Hippo
Keyword(s):  
Gene Delivery ◽  
Stable Gene ◽  
Intestinal Organoids

Sleeping BeautyTransposon-Mediated Stable Gene Delivery

2011 ◽  
pp. 135-161
Author(s):  
Andrew Wilber ◽  
Xianzheng Zhou ◽  
Xin Huang ◽  
Dan S. Kaufman ◽  
R. Scott McIvor
Keyword(s):  
Gene Delivery ◽  
Stable Gene

Stable Gene Delivery to CNS Cells Using Lentiviral Vectors

2004 ◽  
pp. 413-428
Author(s):  
Deborah J. Watson ◽  
Brian A. Karolewski ◽  
John H. Wolfe
Keyword(s):  
Gene Delivery ◽  
Lentiviral Vectors ◽  
Stable Gene

Targeted and Stable Gene Delivery into Muscle Cells by a Two-Step Transfer System

2000 ◽  
Vol 275 (3) ◽  
pp. 931-935 ◽  
Author(s):  
Isao Fujii ◽  
Satoru Suzuki ◽  
Takehito Igarashi ◽  
Makoto Matsukura ◽  
Teruhisa Miike ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Muscle Cells ◽  
Stable Gene ◽  
Transfer System

Efficient construction of stable gene nanoparticles through polymerase chain reaction with flexible branched primers for gene delivery

2015 ◽  
Vol 51 (44) ◽  
pp. 9208-9211 ◽  
Author(s):  
Jianbing Liu ◽  
Runyu Wang ◽  
Dejun Ma ◽  
Di Ouyang ◽  
Zhen Xi
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Delivery ◽  
Stable Gene ◽  
Chain Reaction ◽  
Efficient Construction ◽  
Polymerase Chain

Flexible branched primers were designed to construct stable gene nanoparticles through polymerase chain reaction for gene delivery.

scholarly journals In Vivo Piggybac-Based Gene Delivery towards Murine Pancreatic Parenchyma Confers Sustained Expression of Gene of Interest

2019 ◽  
Vol 20 (13) ◽  
pp. 3116 ◽  
Author(s):  
Masahiro Sato ◽  
Emi Inada ◽  
Issei Saitoh ◽  
Shingo Nakamura ◽  
Satoshi Watanabe
Keyword(s):  
Gene Expression ◽  
Gene Delivery ◽  
Fluorescent Protein ◽  
Consensus Sequence ◽  
Constitutive Expression ◽  
Endocrine System ◽  
Stable Gene ◽  
Pancreatic Cells ◽  
Glandular Organ

The pancreas is a glandular organ that functions in the digestive system and endocrine system of vertebrates. The most common disorders involving the pancreas are diabetes, pancreatitis, and pancreatic cancer. In vivo gene delivery targeting the pancreas is important for preventing or curing such diseases and for exploring the biological function of genes involved in the pathogenesis of these diseases. Our previous experiments demonstrated that adult murine pancreatic cells can be efficiently transfected by exogenous plasmid DNA following intraparenchymal injection and subsequent in vivo electroporation using tweezer-type electrodes. Unfortunately, the induced gene expression was transient. Transposon-based gene delivery, such as that facilitated by piggyBac (PB), is known to confer stable integration of a gene of interest (GOI) into host chromosomes, resulting in sustained expression of the GOI. In this study, we investigated the use of the PB transposon system to achieve stable gene expression when transferred into murine pancreatic cells using the above-mentioned technique. Expression of the GOI (coding for fluorescent protein) continued for at least 1.5 months post-gene delivery. Splinkerette-PCR-based analysis revealed the presence of the consensus sequence TTAA at the junctional portion between host chromosomes and the transgenes; however, this was not observed in all samples. This plasmid-based PB transposon system enables constitutive expression of the GOI in pancreas for potential therapeutic and biological applications.

Flexible DNA junction assisted efficient construction of stable gene nanoparticles for gene delivery

2016 ◽  
Vol 52 (9) ◽  
pp. 1953-1956 ◽  
Author(s):  
Jianbing Liu ◽  
Yanyan Li ◽  
Dejun Ma ◽  
Di Ouyang ◽  
Zhen Xi
Keyword(s):  
Gene Delivery ◽  
Eukaryotic Cells ◽  
Stable Gene ◽  
Efficient Gene ◽  
Efficient Construction

A flexible DNA junction was designed to construct stable gene nanoparticles, which can be used as efficient gene cargo for eukaryotic cells.

scholarly journals Syncytins enable novel possibilities to transduce human or mouse primary B cells and to achieve well-tolerated in vivo gene transfer

2019 ◽  
Author(s):  
Y. Coquin ◽  
M. Ferrand ◽  
A. Seye ◽  
L. Menu ◽  
A. Galy
Keyword(s):  
T Cells ◽  
B Cells ◽  
Gene Transfer ◽  
Gene Delivery ◽  
B Cell ◽  
Stable Gene ◽  
Murine Bone Marrow ◽  
High Level

AbstractSyncytins are cellular transmembrane glycoproteins with fusogenic and immunosuppressive properties that are encoded by endogenous retroviral envelope sequences in mammalian genomes. Based on their properties, syncytins may be useful to pseudotype lentiviral gene transfer vectors (LV) and to obtain well-tolerated in vivo gene delivery but their cellular targets are unknown in this context. We pseudotyped LV with human or murine syncytins. Such LV-Syn particles were infectious in vitro but required a transduction additive, as do other retroviral envelope LV pseudotypes. In these conditions, LV-Syn remarkably transduced quiescent human or murine primary B cells at high level in vitro including naïve blood B cells or B cell precursors from murine bone marrow. Transduced human B cells could be expanded in culture and were functional. Human or murine T cells were transduced less efficiently than B cells, in agreement with lower levels of syncytin receptors on T cells compared to B cells. Well-tolerated in vivo gene transfer was possible without additive, as demonstrated with murine syncytin A-mediated gene delivery in C57BL/6 mice. A single intravenous injection of LV-SynA vector to mice led to stable gene transfer into spleen germinal center B cells. LV-SynA were also intrinsically less immunogenic than LV-VSVG, leading to low antibody responses against the vector capsid. This is the first evidence of interactions between syncytins and B cells, providing novel opportunities for B cell genetic engineering and for well-tolerated gene transfer in vivo. The findings also suggest that some immunosuppressive properties of syncytins could be mediated by B cells.One Sentence SummarySyncytins are fusogenic cellular proteins that can pseudotype lentiviral gene transfer vector particles, achieving efficient gene transfer into primary quiescent B cells and reducing the in vivo immunogenicity of the particles following systemic administration.

1004: In-Vivo Transfection of the Rat Corpus Cavernosum Using a Non-Viral, Linear Polyethylenimine Gene Delivery System: A Novel Application

2006 ◽  
Vol 175 (4S) ◽  
pp. 323-324 ◽  
Author(s):  
Joseph Dall'era ◽  
Sweaty Koul ◽  
Jesse Mills ◽  
Jeremy Myers ◽  
Randall B. Meacham ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Corpus Cavernosum ◽  
Gene Delivery System ◽  
System A ◽  
In Vivo Transfection ◽  
Linear Polyethylenimine
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