Qualitative and Quantitative Determination of Quorum Sensing Inhibition In Vitro

Author(s):  
Tim Holm Jakobsen ◽  
Maria Alhede ◽  
Louise Dahl Hultqvist ◽  
Thomas Bjarnsholt ◽  
Michael Givskov
Author(s):  
Tim Holm Jakobsen ◽  
Maria van Gennip ◽  
Louise Dahl Christensen ◽  
Thomas Bjarnsholt ◽  
Michael Givskov

2019 ◽  
Vol 70 (7) ◽  
pp. 2519-2523
Author(s):  
Denisa Batir Marin ◽  
Oana Cioanca ◽  
Mihai Apostu ◽  
Cristina Gabriela Tuchilus ◽  
Cornelia Mircea ◽  
...  

The objective of the current study is represented by the determination of silica and a phytochemical screening of phenolic derivates of some Equisetum species. The antioxidant and antimicrobial activity for Equisetum pratense Ehrh.,, Equisetum sylvaticum L. and Equisetum telmateia Ehrh. (sin. Equisetum maximum Lam.) were also investigated. The concentration of silicon (Si) in plants was determined by the spectrophotometric method using previous treatment with NaOH 50% both for the stem and the nodal branches [1]. Results obtained varied from 95.12 to 162.10 SiO2 mg/g dry plant which represents 4.44% to 7.58% Si/100g dry sample. Two types of total extracts were obtained using different solvents and were subjected to qualitative and quantitative chemical analysis considering total phenolic content [2]. The highest concentration of investigated compounds was found in the methanolic extract, E. sylvaticum, 196.5mg/g dry sample. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC50 (�g/mL) [3]. Values gathered ranged from 261.7 to 429.5 �g/mL. The highest capacity to neutralized DPPH radicals was found in E. sylvaticum. In vitro antimicrobial activity was determined using difusimetric method [4]. Testing was performed on four microorganisms: three strains of bacteria and one species of fungi. Different effects were noticed against the bacteria, furthermore the methanol extract appeared to be most efficient. All extracts showed significand antimicrobial activity against Staphylococcus aureus (ATCC 25923) and Candida albicans (ATCC 90028) and weak to no activity against Pseudomonas aeruginosa (ATCC 27853) and Escherichia coli (ATCC 25922).


2021 ◽  
Author(s):  
Yiwen Cao ◽  
Shenjie Zhu ◽  
Lin Zhang ◽  
Qun Cui ◽  
Haiyan Wang

Aiming at the difficulty in qualitative and quantitative analysis of trace compositions in food preservative propionic acid, the trace compositions and the key components influencing the total aldehyde content in...


1987 ◽  
Vol 20 (4) ◽  
pp. 290-291
Author(s):  
M. Moss ◽  
C.S.Y. Wong ◽  
T. McMahon ◽  
C. Cousins ◽  
C. Jacklyn

2011 ◽  
Vol 66 (1) ◽  
pp. 102-108 ◽  
Author(s):  
Shihu Wei ◽  
Peicheng Zhang ◽  
Xizhi Feng ◽  
Hiroyuki Kodama ◽  
Changyuan Yu ◽  
...  

Author(s):  
BS Dattilo ◽  
S Gallo ◽  
G Lionetti ◽  
SG Rossi

AbstractA new method is described for the qualitative and quantitative determination of both free and bound maleic hydrazide residues in tobacco leaves and cigarette filler by high performance liquid chromatography. Analyses were carried out by hydrolyzing samples of ground tobacco with 4 N hydrochloric acid for 40 minutes under reflux followed by sample chromatography, running isocratic elutions with a dilute solution of phosphoric acid. The quantitative determination of maleic hydrazide was performed by light absorption at 320 nm, by the calibration curve method. Recoveries of maleic hydrazide added to tobacco samples were greater than 90 %. The detection limit of the method, determined on ground tobacco leaves, was at least 5 ppm. The results obtained by this procedure and by the ISO standard method no. 4876 are in good accordance.


2010 ◽  
Vol 878 (29) ◽  
pp. 2943-2948 ◽  
Author(s):  
Shuli Man ◽  
Wenyuan Gao ◽  
Yanjun Zhang ◽  
Jieyin Wang ◽  
Wanshun Zhao ◽  
...  

2018 ◽  
Vol 8 (6-s) ◽  
pp. 182-186
Author(s):  
Vineeta Singh ◽  
Anita Rao ◽  
Shipra Pandey ◽  
Vaibhav Sharan Pandey ◽  
Vageshwari Vageshwari ◽  
...  

The present enquiry was intended to analyze the phytochemicals qualitatively and quantitatively from flowers of Spanish cherry tree. Flower powder was extracted using polar and nonpolar solvent by soxhlet apparatus. Percentage yield of crude extracts was determined and further the extracts were subjected to analyze the phytochemicals qualitatively and quantitatively by standard procedure. Qualitative analysis showed the absence of alkaloids while presences of tannins, saponins, terpenoids, steroids, glycosides, flavonoids, phenols. Quantitative estimation of phytochemicals was determined using standard curve. Result revealed that the tannin content was 4.3±0.01 (mgTAE /gm), flavanols content was 0.28±0.05 (mgQE/gm). Saponins content was 3.6±0.7 % and terpenoids content was 1.47±0.37 %. A well conducted studies on phytochemicals revealed that they are vital for humans because they provide protection against a variety of ailments. Therefore, the present study is aimed to analyze phytochemicals qualitatively and quantitatively. Keywords: Phytochemicals, Tannins, Saponins, Flavanols, Terpenoids


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