Establishment of Two Dimensional (2D) and Three-Dimensional (3D) Melanoma Primary Cultures as a Tool for In Vitro Drug Resistance Studies

2019 ◽  
pp. 119-131 ◽  
Author(s):  
Nataly Cruz Rodríguez ◽  
Johanna Lineros ◽  
Carol Stefany Rodríguez ◽  
Lina María Martínez ◽  
Josefa Antonia Rodríguez
Keyword(s):  
Drug Resistance ◽  
Primary Cultures ◽  
Three Dimensional ◽  
Two Dimensional

scholarly journals Recapitulating tumour microenvironment in chitosan–gelatin three-dimensional scaffolds: an improved in vitro tumour model

2012 ◽  
Vol 9 (77) ◽  
pp. 3288-3302 ◽  
Author(s):  
Neha Arya ◽  
Viren Sardana ◽  
Meera Saxena ◽  
Annapoorni Rangarajan ◽  
Dhirendra S. Katti
Keyword(s):  
Cancer Progression ◽  
Expression Profiles ◽  
Three Dimensional ◽  
Gene Expression Profiles ◽  
Petri Dish ◽  
Tumour Microenvironment ◽  
Two Dimensional ◽  
Tumour Model

Owing to the reduced co-relationship between conventional flat Petri dish culture (two-dimensional) and the tumour microenvironment, there has been a shift towards three-dimensional culture systems that show an improved analogy to the same. In this work, an extracellular matrix (ECM)-mimicking three-dimensional scaffold based on chitosan and gelatin was fabricated and explored for its potential as a tumour model for lung cancer. It was demonstrated that the chitosan–gelatin (CG) scaffolds supported the formation of tumoroids that were similar to tumours grown in vivo for factors involved in tumour-cell–ECM interaction, invasion and metastasis, and response to anti-cancer drugs. On the other hand, the two-dimensional Petri dish surfaces did not demonstrate gene-expression profiles similar to tumours grown in vivo . Further, the three-dimensional CG scaffolds supported the formation of tumoroids, using other types of cancer cells such as breast, cervix and bone, indicating a possible wider potential for in vitro tumoroid generation. Overall, the results demonstrated that CG scaffolds can be an improved in vitro tool to study cancer progression and drug screening for solid tumours.

Associated Anisotropy of intrinsic NAD(P)H for monitoring changes in the metabolic activities of breast cancer cells in three-dimensional collagen matrix

2021 ◽  
Author(s):  
Anh Cong ◽  
Rafaela M. L. Pimenta ◽  
Jon Holy ◽  
Ahmed A Heikal
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Breast Cancer Cells ◽  
Monolayer Culture ◽  
Three Dimensional ◽  
Collagen Matrix ◽  
Living Cells ◽  
Two Dimensional ◽  
Metabolic Activities

The majority of in vitro studies of living cells are routinely conducted in a two-dimensional (2D) monolayer culture. Recent studies, however, suggest that 2D cell culture promotes specific types of...

Micro-CT analysis of two-dimensional and three-dimensional parameters in severely curved roots prepared with 3 instrumentation systems: An in vitro study

2020 ◽  
Vol 9 (2) ◽  
pp. 62
Author(s):  
AntonioMiranda da Cruz-Filho ◽  
LuisEduardo Souza-Flamini ◽  
BrunoMonguilhott Crozeta ◽  
RicardoGariba Silva ◽  
RicardoNovak Savioli ◽  
...  
Keyword(s):  
Three Dimensional ◽  
In Vitro Study ◽  
Vitro Study ◽  
Two Dimensional ◽  
Micro Ct ◽  
Ct Analysis ◽  
Dimensional Parameters

Three-Dimensional Volumetric Ultrasound Imaging of Arterial Pathology from Two-Dimensional Intravascular Ultrasound: An in Vitro Study

Angiology ◽  
1994 ◽  
Vol 45 (4) ◽  
pp. 253-264 ◽  
Author(s):  
Krishnaswamy Chandrasekaran ◽  
Chandra M. Sehgal ◽  
Tsui-Lieh Hsu ◽  
Nancy A. Young ◽  
Arthur J. D'Adamo ◽  
...  
Keyword(s):  
Intravascular Ultrasound ◽  
Ultrasound Imaging ◽  
Three Dimensional ◽  
In Vitro Study ◽  
Vitro Study ◽  
Two Dimensional ◽  
Volumetric Ultrasound

scholarly journals A three-dimensional model of the human blood-brain barrier to analyse the transport of nanoparticles and astrocyte/endothelial interactions

F1000Research ◽  
2016 ◽  
Vol 4 ◽  
pp. 1279 ◽  
Author(s):  
Peddagangannagari Sreekanthreddy ◽  
Radka Gromnicova ◽  
Heather Davies ◽  
James Phillips ◽  
Ignacio A. Romero ◽  
...  
Keyword(s):  
Blood Brain Barrier ◽  
Human Blood ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Cell Types ◽  
Brain Barrier ◽  
Two Dimensional ◽  
Three Dimensional Model ◽  
Blood Brain

The aim of this study was to develop a three-dimensional (3D) model of the human blood-brain barrier in vitro, which mimics the cellular architecture of the CNS and could be used to analyse the delivery of nanoparticles to cells of the CNS. The model includes human astrocytes set in a collagen gel, which is overlaid by a monolayer of human brain endothelium (hCMEC/D3 cell line). The model was characterised by transmission electron microscopy (TEM), immunofluorescence microscopy and flow cytometry. A collagenase digestion method could recover the two cell types separately at 92-96% purity.  Astrocytes grown in the gel matrix do not divide and they have reduced expression of aquaporin-4 and the endothelin receptor, type B compared to two-dimensional cultures, but maintain their expression of glial fibrillary acidic protein. The effects of conditioned media from these astrocytes on the barrier phenotype of the endothelium was compared with media from astrocytes grown conventionally on a two-dimensional (2D) substratum. Both induce the expression of tight junction proteins zonula occludens-1 and claudin-5 in hCMEC/D3 cells, but there was no difference between the induced expression levels by the two media. The model has been used to assess the transport of glucose-coated 4nm gold nanoparticles and for leukocyte migration. TEM was used to trace and quantitate the movement of the nanoparticles across the endothelium and into the astrocytes. This blood-brain barrier model is very suitable for assessing delivery of nanoparticles and larger biomolecules to cells of the CNS, following transport across the endothelium.

scholarly journals Amyloid fibril-based hydrogels for high-throughput tumor spheroid modeling

2020 ◽  
Author(s):  
Namrata Singh ◽  
Komal Patel ◽  
Ambuja Navalkar ◽  
Pradeep Kadu ◽  
Debalina Datta ◽  
...  
Keyword(s):  
Drug Resistance ◽  
High Throughput ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Cancer Therapeutics ◽  
Cost Effective ◽  
Cell Types ◽  
Tumor Spheroid ◽  
Tumor Modeling

AbstractBiomaterials mimicking extracellular matrices (ECM) for three-dimensional (3D) cultures have gained immense interest in tumor modeling and in vitro organ development. Here, we introduce versatile, thixotropic amyloid hydrogels as a bio-mimetic ECM scaffold for 3D cell culture as well as high-throughput tumor spheroid formation using a drop cast method. The unique cross-β-sheet structure, sticky surface, and thixotropicity of amyloid hydrogels allow robust cell adhesion, survival, proliferation, and migration, which are essential for 3D tumor modeling with various cancer cell types. The spheroids formed show overexpression of the signature cancer biomarkers and confer higher drug resistance compared to two-dimensional (2D) monolayer cultures. Using breast tumor tissue from mouse xenograft, we showed that these hydrogels support the formation of tumor spheroids with a well-defined necrotic core, cancer-associated gene expression, higher drug resistance, and tumor heterogeneity reminiscent of the original tumor. Altogether, we have developed a rapid and cost-effective platform for generating in vitro cancer models for the screening of anti-cancer therapeutics and developing personalized medicines.

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