The in vitro biocompatibility of d -(+) raffinose modified chitosan: Two-dimensional and three-dimensional systems for culturing of horse articular chondrocytes

Owing to the reduced co-relationship between conventional flat Petri dish culture (two-dimensional) and the tumour microenvironment, there has been a shift towards three-dimensional culture systems that show an improved analogy to the same. In this work, an extracellular matrix (ECM)-mimicking three-dimensional scaffold based on chitosan and gelatin was fabricated and explored for its potential as a tumour model for lung cancer. It was demonstrated that the chitosan–gelatin (CG) scaffolds supported the formation of tumoroids that were similar to tumours grown in vivo for factors involved in tumour-cell–ECM interaction, invasion and metastasis, and response to anti-cancer drugs. On the other hand, the two-dimensional Petri dish surfaces did not demonstrate gene-expression profiles similar to tumours grown in vivo . Further, the three-dimensional CG scaffolds supported the formation of tumoroids, using other types of cancer cells such as breast, cervix and bone, indicating a possible wider potential for in vitro tumoroid generation. Overall, the results demonstrated that CG scaffolds can be an improved in vitro tool to study cancer progression and drug screening for solid tumours.

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Silicate-doped nano-hydroxyapatite/graphene oxide composite reinforced fibrous scaffolds for bone tissue engineering

Journal of Biomaterials Applications ◽

10.1177/0885328218763665 ◽

2018 ◽

Vol 32 (10) ◽

pp. 1392-1405 ◽

Cited By ~ 15

Author(s):

Ali Deniz Dalgic ◽

Ammar Z. Alshemary ◽

Ayşen Tezcaner ◽

Dilek Keskin ◽

Zafer Evis

Keyword(s):

Tissue Engineering ◽

Graphene Oxide ◽

Bone Tissue Engineering ◽

Protein Adsorption ◽

Bone Tissue ◽

Three Dimensional ◽

Osteosarcoma Cell ◽

In Vitro Biocompatibility ◽

Microscopy Techniques

In this study, novel graphene oxide–incorporated silicate-doped nano-hydroxyapatite composites were prepared and their potential use for bone tissue engineering was investigated by developing an electrospun poly(ε-caprolactone) scaffold. Nanocomposite groups were synthesized to have two different ratios of graphene oxide (2 and 4 wt%) to evaluate the effect of graphene oxide incorporation and groups with different silicate-doped nano-hydroxyapatite content was prepared to investigate optimum concentrations of both silicate-doped nano-hydroxyapatite and graphene oxide. Three-dimensional poly(ε-caprolactone) scaffolds were prepared by wet electrospinning and reinforced with silicate-doped nano-hydroxyapatite/graphene oxide nanocomposite groups to improve bone regeneration potency. Microstructural and chemical characteristics of the scaffolds were investigated by X-ray diffraction, Fourier transform infrared spectroscope and scanning electron microscopy techniques. Protein adsorption and desorption on material surfaces were studied using fetal bovine serum. Presence of graphene oxide in the scaffold, dramatically increased the protein adsorption with decreased desorption. In vitro biocompatibility studies were conducted using human osteosarcoma cell line (Saos-2). Electrospun scaffold group that was prepared with effective concentrations of silicate-doped nano-hydroxyapatite and graphene oxide particles (poly(ε-caprolactone) – 10% silicate-doped nano-hydroxyapatite – 4% graphene oxide) showed improved adhesion, spreading, proliferation and alkaline phosphatase activity compared to other scaffold groups.

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Associated Anisotropy of intrinsic NAD(P)H for monitoring changes in the metabolic activities of breast cancer cells in three-dimensional collagen matrix

Physical Chemistry Chemical Physics ◽

10.1039/d0cp06635d ◽

2021 ◽

Author(s):

Anh Cong ◽

Rafaela M. L. Pimenta ◽

Jon Holy ◽

Ahmed A Heikal

Keyword(s):

Breast Cancer ◽

Cell Culture ◽

Breast Cancer Cells ◽

Monolayer Culture ◽

Three Dimensional ◽

Collagen Matrix ◽

Living Cells ◽

Two Dimensional ◽

Metabolic Activities

The majority of in vitro studies of living cells are routinely conducted in a two-dimensional (2D) monolayer culture. Recent studies, however, suggest that 2D cell culture promotes specific types of...

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Micro-CT analysis of two-dimensional and three-dimensional parameters in severely curved roots prepared with 3 instrumentation systems: An in vitro study

European Journal of General Dentistry ◽

10.4103/ejgd.ejgd_140_19 ◽

2020 ◽

Vol 9 (2) ◽

pp. 62

Author(s):

AntonioMiranda da Cruz-Filho ◽

LuisEduardo Souza-Flamini ◽

BrunoMonguilhott Crozeta ◽

RicardoGariba Silva ◽

RicardoNovak Savioli ◽

...

Keyword(s):

Three Dimensional ◽

In Vitro Study ◽

Vitro Study ◽

Two Dimensional ◽

Micro Ct ◽

Ct Analysis ◽

Dimensional Parameters

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Injectable Alginate-Peptide Composite Hydrogel as a Scaffold for Bone Tissue Regeneration

Nanomaterials ◽

10.3390/nano9040497 ◽

2019 ◽

Vol 9 (4) ◽

pp. 497 ◽

Cited By ~ 20

Author(s):

Moumita Ghosh ◽

Michal Halperin-Sternfeld ◽

Itzhak Grinberg ◽

Lihi Adler-Abramovich

Keyword(s):

Extracellular Matrix ◽

Bone Regeneration ◽

Composite Scaffold ◽

Three Dimensional ◽

Composite Hydrogel ◽

Bone Tissue Regeneration ◽

Pcr Analysis ◽

Alizarin Red ◽

In Vitro Biocompatibility

The high demand for tissue engineering scaffolds capable of inducing bone regeneration using minimally invasive techniques prompts the need for the development of new biomaterials. Herein, we investigate the ability of Alginate incorporated with the fluorenylmethoxycarbonyl-diphenylalanine (FmocFF) peptide composite hydrogel to serve as a potential biomaterial for bone regeneration. We demonstrate that the incorporation of the self-assembling peptide, FmocFF, in sodium alginate leads to the production of a rigid, yet injectable, hydrogel without the addition of cross-linking agents. Scanning electron microscopy reveals a nanofibrous structure which mimics the natural bone extracellular matrix. The formed composite hydrogel exhibits thixotropic behavior and a high storage modulus of approximately 10 kPA, as observed in rheological measurements. The in vitro biocompatibility tests carried out with MC3T3-E1 preosteoblast cells demonstrate good cell viability and adhesion to the hydrogel fibers. This composite scaffold can induce osteogenic differentiation and facilitate calcium mineralization, as shown by Alizarin red staining, alkaline phosphatase activity and RT-PCR analysis. The high biocompatibility, excellent mechanical properties and similarity to the native extracellular matrix suggest the utilization of this hydrogel as a temporary three-dimensional cellular microenvironment promoting bone regeneration.

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Titanium Oxide (TiO2) Coatings Produced on Titanium by Oxygen-Plasma Immersion and Cell Behaviour on TiO2

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.309-311.367 ◽

2006 ◽

Vol 309-311 ◽

pp. 367-370 ◽

Cited By ~ 4

Author(s):

E.T. Uzumaki ◽

A.R. Santos ◽

C.S. Lambert

Keyword(s):

Titanium Oxide ◽

Oxygen Plasma ◽

Three Dimensional ◽

Titanium Substrate ◽

X Ray Diffraction ◽

Cell Behaviour ◽

In Vitro Biocompatibility ◽

Metallic Implants ◽

Titanium Substrates

Plasma immersion process was investigated as a method for producing bioceramics coatings on metallic implants due to its advantages, which include the production of coatings on three-dimensional workpieces, with high density and superior adhesion. In this process, the oxygen plasma was utilized to form titanium oxide on titanium substrate. The structure, composition and surface morphology were studied using scanning electron microscopy (SEM) and X-ray diffraction. In addition a preliminary study has also been carried out, on TiO2-coated and uncoated titanium substrates, to analyse the in vitro biocompatibility (cytotoxicity evaluation and cell morphology).

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