Discovering Protein Biomarkers from Clinical Peripheral Blood Mononuclear Cells Using Data-Independent Acquisition Mass Spectrometry

2019 ◽  
pp. 151-161
Author(s):  
Xin Ku ◽  
Wei Yan
Keyword(s):  
Mass Spectrometry ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Protein Biomarkers ◽  
Peripheral Blood Mononuclear ◽  
Data Independent Acquisition ◽  
Blood Mononuclear Cells ◽  
Using Data

scholarly journals Quantitative Analysis of Antiretroviral Drugs in Lysates of Peripheral Blood Mononuclear Cells Using MALDI-Triple Quadrupole Mass Spectrometry

2008 ◽  
Vol 80 (13) ◽  
pp. 4969-4975 ◽  
Author(s):  
Jeroen J. A. van Kampen ◽  
Peter C. Burgers ◽  
Rob A. Gruters ◽  
Albert D. M. E Osterhaus ◽  
Ronald de Groot ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Antiretroviral Drugs ◽  
Triple Quadrupole ◽  
Quadrupole Mass ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Inductively coupled plasma mass spectrometry method for plasma and intracellular antimony quantification applied to pharmacokinetics of meglumine antimoniate

Bioanalysis ◽  
2021 ◽  
Author(s):  
Diana J Garay-Baquero ◽  
David E Rebellón-Sánchez ◽  
Miguel D Prieto ◽  
Lina Giraldo-Parra ◽  
Adriana Navas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Human Plasma ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Inductively Coupled Plasma ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Inductively Coupled ◽  
Blood Mononuclear Cells ◽  
Plasma Mass Spectrometry

Background: A high-throughput method using inductively coupled plasma mass spectrometry (ICP–MS) was developed and validated for the quantitative analysis of antimony in human plasma and peripheral blood mononuclear cells from patients with cutaneous leishmaniasis undergoing treatment with meglumine antimoniate. Materials & methods: Antimony was digested in clinical samples with 1% tetramethylammonium hydroxide/1% EDTA and indium was used as internal standard. Accuracy, precision and stability were evaluated. Conclusion: Taking the lower limit of quantitation to be the lowest validation concentration with precision and accuracy within 20%, the current assay was successfully validated from 25 to 10000 ng/ml for antimony in human plasma and peripheral blood mononuclear cells. This protocol will serve as a baseline for future analytical designs, aiming to provide a reference method to allow inter-study comparisons.

scholarly journals Colonic Bacteria-Transformed Catechin Metabolite Response to Cytokine Production by Human Peripheral Blood Mononuclear Cells

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 830 ◽  
Author(s):  
Rajapandiyan Krishnamoorthy ◽  
Abdulraheem R. Adisa ◽  
Vaiyapuri Subbarayan Periasamy ◽  
Jegan Athinarayanan ◽  
Subash-Babu Pandurangan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Immune Response ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Peripheral Blood Mononuclear ◽  
Chromatography Mass Spectrometry ◽  
Blood Mononuclear Cells ◽  
Anti Inflammatory

Human gut microbes are a profitable tool for the modification of food compounds into biologically active metabolites. The biological properties of catechins have been extensively investigated. However, the bioavailability of catechin in human blood plasma is very low. This study aimed to determine the biotransformed catechin metabolites and their bioactive potentials for modulating the immune response of human peripheral blood mononuclear cells (PBMCs). Biotransformation of catechin was carried out using in-vitro gut microbial biotransformation method, the transformed metabolites were identified and confirmed by gas chromatography-mass spectrometry (GC–MS) and high-performance liquid chromatography-mass spectrometry (HPLC–MS). Present observations confirmed that the catechin was biotransformed into 11 metabolites upon microbial dehydroxylation and C ring cleavage. Further, immunomodulatory potential of catechin metabolites was analyzed in peripheral blood mononuclear cells (PBMCs). We found up-regulation of anti-inflammatory cytokine (IL-4, IL-10) and down-regulation of pro-inflammatory (IL-16, IL-12B) cytokine may be due to Th2 immune response. In conclusion, biotransformed catechin metabolites enhance anti-inflammatory cytokines which is beneficial for overcoming inflammatory disorders.

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