Genetic Engineering Approaches Used to Increase Lipid Production and Alter Lipid Profile in Microbes

2019 ◽  
pp. 141-150
Author(s):  
Xiao-Ling Tang ◽  
Ya-Ping Xue
Keyword(s):  
Genetic Engineering ◽  
Lipid Profile ◽  
Lipid Production

scholarly journals Genetic engineering of microalgae lipid biosynthesis for sustainable biodiesel production

2021 ◽  
Vol 11 (3) ◽  
pp. 072-077
Author(s):  
Siti Zulaiha
Keyword(s):  
Genetic Engineering ◽  
Metabolic Pathways ◽  
Alternative Energy ◽  
Fossil Fuels ◽  
Lipid Production ◽  
High Growth ◽  
Lipid Biosynthesis ◽  
Production Costs ◽  
Biodiesel Production ◽  
Promising Alternative

Biofuel is one of the most promising alternative energy sources for reducing human reliance on fossil fuels. Microalgae has recently emerged as the most promising biofuel source. However, biofuels from microalgae are still not feasible to replace fossil fuels because of their high production costs, therefore, it is necessary to pick microalgae species with high growth rates and lipid content. Overexpression of lipid biosynthesis enzymes and inhibition of competitive metabolic pathways are two genetic engineering strategies that can be developed to assess microalgae lipid production. Malate and multienzyme enzymes (GPAT, LPAAT and DGAT) can be overexpressed in microalgae to boost lipid production. The strategy of blocking competitive metabolic pathways can be carried out through suppression of starch metabolism and lipid catabolism. The strategy of blocking competitive metabolic pathways has been carried out in several microalgae and is effective for enhancing lipid biosynthesis. Several mutations that block both the starch metabolic and lipid catabolic pathways can result in increased levels of microalgal lipid accumulation.

Genetic Engineering Tools for Enhancing Lipid Production in Microalgae

2015 ◽  
pp. 119-127
Author(s):  
Sheena Kumari ◽  
Poonam Singh ◽  
Sanjay Kumar Gupta ◽  
Santhosh Kumar
Keyword(s):  
Genetic Engineering ◽  
Lipid Production

scholarly journals A selective microalgae strain for biodiesel production in relation to higher lipid profile

2019 ◽  
Vol 1 (1) ◽  
pp. 8-14
Author(s):  
Prakash Bhuyar ◽  
Mohd Hasbi Ab. Rahim ◽  
Mashitah M. Yusoff ◽  
Gaanty Pragas Maniam ◽  
Natanamurugaraj Govindan
Keyword(s):  
Lipid Profile ◽  
Lipid Analysis ◽  
Lipid Production ◽  
Lipid Extraction ◽  
Peninsular Malaysia ◽  
Climatic Conditions ◽  
Biodiesel Production ◽  
High Lipid ◽  
Photosynthetic Organism ◽  
Short Time

Biodiesel have become the important asset by the country especially to build up their economy. Currently, microalgae have been choosing as the source for production of biodiesel based on their advantages. Microalgae are a photosynthetic organism that use light as an energy source and able to produce their own food. These microalgae also produce a lipid that can be used to produce a biodiesel. Using microalgae that contain high lipid profile are very important to make sure the biodiesel can be produce in large quantity in short time and more cost saving. Although many microalgae species have been identified and isolated for lipid production, there is currently no consensus as to which species provide the highest productivity. Different species are expected to function best at different aquatic, geographical and climatic conditions. So, this experiment is conducted to identify which strain of microalgae contains high lipid profile that can be used to convert into the biodiesel. There are three main objectives that involve in this experiment which is to isolate and identify different strain of microalgae from Kuantan Coast, East Coast Peninsular Malaysia, to convert the lipid from microalgae into biodiesel through transesterification, and to estimate higher lipid profile of microalgae species for biodiesel production. Two species of green microalgae were isolated, which is Nannochloropsis sp and Coelastrum sp. Based on lipid extraction and lipid analysis, it shows that the Nannochloropsis sp. have more concentrated of lipid and higher lipid profile compared to Coelastrum sp. Hence, Nannochloropsis sp. are most suitable species that can be used as a biodiesel feedstock due to higher lipid profile of MUFA.

scholarly journals Genetic engineering of microalgae for enhanced lipid production

2021 ◽  
pp. 107836
Author(s):  
Camilo F. Muñoz ◽  
Christian Südfeld ◽  
Mihris I.S. Naduthodi ◽  
Ruud A. Weusthuis ◽  
Maria J. Barbosa ◽  
...  
Keyword(s):  
Genetic Engineering ◽  
Lipid Production

scholarly journals Genetic engineering, a hope for sustainable biofuel production: review

2014 ◽  
Vol 3 (2) ◽  
pp. 311-323 ◽  
Author(s):  
Sudip Paudel ◽  
Michael A Menze
Keyword(s):  
Genetic Engineering ◽  
Fossil Fuels ◽  
Genetically Modified Organisms ◽  
Lipid Production ◽  
Genetically Engineered ◽  
Raw Material ◽  
Biofuel Production ◽  
Wild Type ◽  
Environment Friendly ◽  
Genetically Engineered Organisms

The use of recently developed genetic engineering tools in combination with organisms that have the potential to produce precursors for the production of biodiesel, promises a sustainable and environment friendly energy source. Enhanced lipid production in wild type and/or genetically engineered organisms can offer sufficient raw material for industrial transesterification of plant-based triglycerides. Bio-diesel, produced with the help of genetically modified organisms, might be one of the best alternatives to fossil fuels and to mitigate various environmental hazards. DOI: http://dx.doi.org/10.3126/ije.v3i2.10644 International Journal of the Environment Vol.3(2) 2014: 311-323

Manipulation of microalgal lipid production using genetic engineering

1996 ◽  
Vol 57-58 (1) ◽  
pp. 223-231 ◽  
Author(s):  
Terri G. Dunahay ◽  
Eric E. Jarvis ◽  
Sonja S. Dais ◽  
Paul G. Roessler
Keyword(s):  
Genetic Engineering ◽  
Lipid Production

scholarly journals Harnessing the Power of Mutagenesis and Adaptive Laboratory Evolution for High Lipid Production by Oleaginous Microalgae and Yeasts

2020 ◽  
Vol 12 (12) ◽  
pp. 5125
Author(s):  
Neha Arora ◽  
Hong-Wei Yen ◽  
George P. Philippidis
Keyword(s):  
Genetic Engineering ◽  
Lipid Production ◽  
Random Mutagenesis ◽  
Strain Improvement ◽  
Production Costs ◽  
Screening Methods ◽  
Scale Production ◽  
Adaptive Laboratory Evolution ◽  
Laboratory Evolution ◽  
Oleaginous Microalgae

Oleaginous microalgae and yeasts represent promising candidates for large-scale production of lipids, which can be utilized for production of drop-in biofuels, nutraceuticals, pigments, and cosmetics. However, low lipid productivity and costly downstream processing continue to hamper the commercial deployment of oleaginous microorganisms. Strain improvement can play an essential role in the development of such industrial microorganisms by increasing lipid production and hence reducing production costs. The main means of strain improvement are random mutagenesis, adaptive laboratory evolution (ALE), and rational genetic engineering. Among these, random mutagenesis and ALE are straight forward, low-cost, and do not require thorough knowledge of the microorganism’s genetic composition. This paper reviews available mutagenesis and ALE techniques and screening methods to effectively select for oleaginous microalgae and yeasts with enhanced lipid yield and understand the alterations caused to metabolic pathways, which could subsequently serve as the basis for further targeted genetic engineering.

Manipulation of Microalgal Lipid Production Using Genetic Engineering

1996 ◽  
pp. 223-231 ◽  
Author(s):  
Terri G. Dunahay ◽  
Eric E. Jarvis ◽  
Sonja S. Dais ◽  
Paul G. Roessler
Keyword(s):  
Genetic Engineering ◽  
Lipid Production

scholarly journals Reducing self-shading effects in Botryococcus braunii cultures: effect of Mg2+ deficiency on optical and biochemical properties, photosynthesis and lipidomic profile

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Néstor David Giraldo ◽  
Sandra Marcela Correa ◽  
Andrés Arbeláez ◽  
Felix L. Figueroa ◽  
Rigoberto Ríos-Estepa ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Neutral Lipids ◽  
Lipid Production ◽  
Biomass Productivity ◽  
Botryococcus Braunii ◽  
Biochemical Properties ◽  
Photosynthetic Performance ◽  
Optimization Approach ◽  
Total Carbohydrate ◽  
Absorption Properties

AbstractMicroalgae biomass exploitation as a carbon–neutral energy source is currently limited by several factors, productivity being one of the most relevant. Due to the high absorption properties of light-harvesting antenna, photosynthetic cells tend to capture an excessive amount of energy that cannot be entirely channeled through the electron transfer chain that ends up dissipated as heat and fluorescence, reducing the overall light use efficiency. Aiming to minimize this hurdle, in this work we studied the effect of decreasing concentrations of Magnesium (Mg2+) on the chlorophyll a content, photosynthetic performance, biomass and lipid production of autotrophic cultures of Botryococcus braunii LB 572. We also performed, for the first time, a comparative lipidomic analysis to identify the influence of limited Mg2+ supply on the lipid profile of this algae. The results indicated that a level of 0.0037 g L−1 MgSO4 caused a significant decline on chlorophyll a content with a concomitant 2.3-fold reduction in the biomass absorption coefficient. In addition, the Mg2+ limitation caused a decrease in the total carbohydrate content and triggered lipid accumulation, achieving levels of up to 53% DCW, whereas the biomass productivity remained similar for all tested conditions. The lipidome analysis revealed that the lowest Mg2+ concentrations also caused a differential lipid profile distribution, with an enrichment of neutral lipids and an increase of structural lipids. In that sense, we showed that Mg2+ limitation represents an alternative optimization approach that not only enhances accumulation of neutral lipids in B. braunii cells but also may potentially lead to a better areal biomass productivity due to the reduction in the cellular light absorption properties of the cells.

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