Analysis of a Transcription Factor Using Transient Assay in Arabidopsis Protoplasts

2011 ◽  
pp. 107-117 ◽  
Author(s):  
Yuji Iwata ◽  
Mi-Hyun Lee ◽  
Nozomu Koizumi
Keyword(s):  
Transcription Factor ◽  
Transient Assay

Transcription factor/DNA interactions visualized by electron spectroscopic imaging

1992 ◽  
Vol 50 (1) ◽  
pp. 450-451
Author(s):  
David P. Bazett-Jones ◽  
Mark L. Brown
Keyword(s):  
Transcription Factor ◽  
Dna Sequences ◽  
Transcription Initiation ◽  
Molecular Mechanisms ◽  
Phosphorus Content ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
Dna Backbone ◽  
Two Parameters ◽  
High Level

A multisubunit RNA polymerase enzyme is ultimately responsible for transcription initiation and elongation of RNA, but recognition of the proper start site by the enzyme is regulated by general, temporal and gene-specific trans-factors interacting at promoter and enhancer DNA sequences. To understand the molecular mechanisms which precisely regulate the transcription initiation event, it is crucial to elucidate the structure of the transcription factor/DNA complexes involved. Electron spectroscopic imaging (ESI) provides the opportunity to visualize individual DNA molecules. Enhancement of DNA contrast with ESI is accomplished by imaging with electrons that have interacted with inner shell electrons of phosphorus in the DNA backbone. Phosphorus detection at this intermediately high level of resolution (≈lnm) permits selective imaging of the DNA, to determine whether the protein factors compact, bend or wrap the DNA. Simultaneously, mass analysis and phosphorus content can be measured quantitatively, using adjacent DNA or tobacco mosaic virus (TMV) as mass and phosphorus standards. These two parameters provide stoichiometric information relating the ratios of protein:DNA content.

The role of transcription factor Egr‐1 in IL‐1 up‐regulation of tubular CD44 expression

Nephrology ◽  
2000 ◽  
Vol 5 (3) ◽  
pp. A92-A92
Author(s):  
Takazoe K ◽  
Foti R ◽  
Hurst La ◽  
Atkins Rc ◽  
Nikolic‐Paterson DJ.
Keyword(s):  
Transcription Factor ◽  
Cd44 Expression

Alpha-fetoprotein producing gastric cancer cells lack transcription factor AT-motif binding factor 1 (ATBF1)

2001 ◽  
Vol 120 (5) ◽  
pp. A31-A31
Author(s):  
H KATAOKA ◽  
T JOH ◽  
T OHSHIMA ◽  
Y ITOH ◽  
K SENOO ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Transcription Factor ◽  
Cancer Cells ◽  
Alpha Fetoprotein ◽  
Gastric Cancer Cells ◽  
Binding Factor

Overexpression of the transcription factor Hand1 causes sudden cardiac death in mice

2008 ◽  
Vol 7 ◽  
pp. 109-109
Author(s):  
R BRECKENRIDGE ◽  
Z ZUBERI ◽  
L FELKIN ◽  
E BIRKS ◽  
P BARTON ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Sudden Cardiac Death ◽  
Cardiac Death

Author response for "Transcription factor binding at immunoglobulin enhancers is linked to somatic hypermutation targeting"

2019 ◽  
Author(s):  
Ravi K. Dinesh ◽  
Benjamin Barnhill ◽  
Anoj Ilanges ◽  
Lizhen Wu ◽  
Daniel A. Michelson ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Somatic Hypermutation ◽  
Transcription Factor Binding ◽  
Author Response ◽  
Factor Binding
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