A New Perspective for the Characterization of Crown Rim Kinematics

2020 ◽  
pp. 163-175
Author(s):  
Ronan Bernard ◽  
Visakh Vaikuntanathan ◽  
Grazia Lamanna ◽  
Bernhard Weigand
Keyword(s):  
New Perspective

scholarly journals Characterization of an Insoluble and Soluble Form of Melanin Produced by Streptomyces cavourensis SV 21, a Sea Cucumber Associated Bacterium

Marine Drugs ◽  
2022 ◽  
Vol 20 (1) ◽  
pp. 54
Author(s):  
Joko Tri Wibowo ◽  
Matthias Y. Kellermann ◽  
Lars-Erik Petersen ◽  
Yustian R. Alfiansah ◽  
Colleen Lattyak ◽  
...  
Keyword(s):  
Biological Properties ◽  
Water Soluble ◽  
Soluble Form ◽  
Wide Range ◽  
Biological Form ◽  
Insoluble Form ◽  
New Perspective ◽  
Living Organisms ◽  
Purification Protocol

Melanin is a widely distributed and striking dark-colored pigment produced by countless living organisms. Although a wide range of bioactivities have been recognized, there are still major constraints in using melanin for biotechnological applications such as its fragmentary known chemical structure and its insolubility in inorganic and organic solvents. In this study, a bacterial culture of Streptomyces cavourensis SV 21 produced two distinct forms of melanin: (1) a particulate, insoluble form as well as (2) a rarely observed water-soluble form. The here presented novel, acid-free purification protocol of purified particulate melanin (PPM) and purified dissolved melanin (PDM) represents the basis for an in-depth comparison of their physicochemical and biological properties, which were compared to the traditional acid-based precipitation of melanin (AM) and to a synthetic melanin standard (SM). Our data show that the differences in solubility between PDM and PPM in aqueous solutions may be a result of different adjoining cation species, since the soluble PDM polymer is largely composed of Mg2+ ions and the insoluble PPM is dominated by Ca2+ ions. Furthermore, AM shared most properties with SM, which is likely attributed to a similar, acid-based production protocol. The here presented gentler approach of purifying melanin facilitates a new perspective of an intact form of soluble and insoluble melanin that is less chemical altered and thus closer to its original biological form.

scholarly journals Characterization of Single-Nucleus Electrical Properties by Microfluidic Constriction Channel

Micromachines ◽  
2019 ◽  
Vol 10 (11) ◽  
pp. 740 ◽  
Author(s):  
Hongyan Liang ◽  
Yi Zhang ◽  
Deyong Chen ◽  
Huiwen Tan ◽  
Yu Zheng ◽  
...  
Keyword(s):  
Unit Area ◽  
Electrical Characterization ◽  
Electrical Model ◽  
Cell Type ◽  
Whole Cells ◽  
Single Nucleus ◽  
Nuclear Isolation ◽  
New Perspective ◽  
Type Classification

As key bioelectrical markers, equivalent capacitance (Cne, i.e., capacitance per unit area) and resistance (Rne, i.e., resistivity multiply thickness) of nuclear envelopes have emerged as promising electrical indicators, which cannot be effectively measured by conventional approaches. In this study, single nuclei were isolated from whole cells and trapped at the entrances of microfluidic constriction channels, and then corresponding impedance profiles were sampled and translated into single-nucleus Cne and Rne based on a home-developed equivalent electrical model. Cne and Rne of A549 nuclei were first quantified as 3.43 ± 1.81 μF/cm2 and 2.03 ± 1.40 Ω·cm2 (Nn = 35), which were shown not to be affected by variations of key parameters in nuclear isolation and measurement. The developed approach in this study was also used to measure a second type of nuclei, producing Cne and Rne of 3.75 ± 3.17 μF/cm2 and 1.01 ± 0.70 Ω·cm2 for SW620 (Nn = 17). This study may provide a new perspective in single-cell electrical characterization, enabling cell type classification and cell status evaluation based on bioelectrical markers of nuclei.

scholarly journals Preparation, Modification, and Characterization of Alginate Hydrogel with Nano-/Microfibers: A New Perspective for Tissue Engineering

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Bianca Palma Santana ◽  
Fernanda Nedel ◽  
Evandro Piva ◽  
Rodrigo Varella de Carvalho ◽  
Flávio Fernando Demarco ◽  
...  
Keyword(s):  
Cell Viability ◽  
Chemical Properties ◽  
Chemical Characteristics ◽  
X Ray Diffraction ◽  
Alginate Hydrogel ◽  
X Ray ◽  
New Perspective ◽  
Nih 3T3

We aimed to develop an alginate hydrogel (AH) modified with nano-/microfibers of titanium dioxide (nfTD) and hydroxyapatite (nfHY) and evaluated its biological and chemical properties. Nano-/microfibers of nfTD and nfHY were combined with AH, and its chemical properties were evaluated by FTIR spectroscopy, X-ray diffraction, energy dispersive X-Ray analysis, and the cytocompatibility by the WST-1 assay. The results demonstrate that the association of nfTD and nfHY nano-/microfibers to AH did not modified the chemical characteristics of the scaffold and that the association was not cytotoxic. In the first 3 h of culture with NIH/3T3 cells nfHY AH scaffolds showed a slight increase in cell viability when compared to AH alone or associated with nfTD. However, an increase in cell viability was observed in 24 h when nfTD was associated with AH scaffold. In conclusion our study demonstrates that the combination of nfHY and nfTD nano-/microfibers in AH scaffold maintains the chemical characteristics of alginate and that this association is cytocompatible. Additionally the combination of nfHY with AH favored cell viability in a short term, and the addition of nfTD increased cell viability in a long term.

A new perspective on the characterization of materials for a-Si:H solar cells

2001 ◽  
Vol 66 (1-4) ◽  
pp. 231-237 ◽  
Author(s):  
L. Jiao ◽  
X. Niu ◽  
Z. Lu ◽  
C.R. Wronski ◽  
A. Matsuda ◽  
...  
Keyword(s):  
Solar Cells ◽  
New Perspective ◽  
Characterization Of Materials

Military Organization Leader Competence

2020 ◽  
Vol 132 (1) ◽  
pp. 65-81
Author(s):  
Dorota Balcerzyk ◽  
Magdalena Zapała
Keyword(s):  
Professional Development ◽  
Military Service ◽  
Officer Training ◽  
Military Organization ◽  
Political Situation ◽  
Army Officer ◽  
The Social ◽  
New Perspective ◽  
Competence Models

The social and political situation driving ongoing changes in the Polish Army is responsible for a new perspective on viewing the competences of a leader in a military organization. The article presents and discusses the issue of professional competences in the context of professional military service. It provides an analysis of selected competence models. The essence of competence is the focus, but at the same time the specificity of the organization—the army—is pointed out. Emphasis is placed on the importance of managerial competences in managerial functions as filled by officers. A characterization of the Polish Army officer training and professional development system is also provided.

Cloning and characterization of a 2,3-oxidosqualene cyclase from Eleutherococcus trifoliatus

Holzforschung ◽  
2013 ◽  
Vol 67 (4) ◽  
pp. 463-471 ◽  
Author(s):  
Li-Ting Ma ◽  
Sheng-Yang Wang ◽  
Yen-Hsueh Tseng ◽  
Yi-Ru Lee ◽  
Fang-Hua Chu
Keyword(s):  
Physiological Role ◽  
Active Site Residues ◽  
Reading Frame ◽  
Oxidosqualene Cyclases ◽  
Oxidosqualene Cyclase ◽  
Acid Protein ◽  
Leaf Tissues ◽  
New Perspective ◽  
Amino Acid Protein

Abstract The 2,3-oxidosqualene cyclases (OSCs) are a family of enzymes that have an important role in plant triterpene biosynthesis. In this study, an OSC gene designed EtLUS from Eleutherococcus trifoliatus has been cloned. EtLUS includes a 2292-bp open reading frame and encodes a 763-amino acid protein. EtLUS has an MLCYCR motif, which is conserved in lupeol synthases. Comparison of active-site residues and gene expression in yeast showed that EtLUS synthesizes lupeol. However, EtLUS has the highest sequence identity with β-amyrin synthases from Araliaceae rather than lupeol synthases, adding new perspective to the evolution of the OSCs of Araliaceae. Furthermore, EtLUS is upregulated in leaf tissues under methyl jasmonate treatment, which can be interpreted that lupeol and its derivatives play an ecological and physiological role in plant defense against pathogens and insect herbivores.

scholarly journals From Fibonacci Sequence to the Golden Ratio

2013 ◽  
Vol 2013 ◽  
pp. 1-3 ◽  
Author(s):  
Alberto Fiorenza ◽  
Giovanni Vincenzi
Keyword(s):  
Characteristic Polynomial ◽  
Golden Ratio ◽  
Fibonacci Sequence ◽  
Maximum Modulus ◽  
Point Of View ◽  
General Point ◽  
Constant Coefficients ◽  
The Difference ◽  
New Perspective

We consider the well-known characterization of the Golden ratio as limit of the ratio of consecutive terms of the Fibonacci sequence, and we give an explanation of this property in the framework of the Difference Equations Theory. We show that the Golden ratio coincides with this limit not because it is the root with maximum modulus and multiplicity of the characteristic polynomial, but, from a more general point of view, because it is the root with maximum modulus and multiplicity of a restricted set of roots, which in this special case coincides with the two roots of the characteristic polynomial. This new perspective is the heart of the characterization of the limit of ratio of consecutive terms of all linear homogeneous recurrences with constant coefficients, without any assumption on the roots of the characteristic polynomial, which may be, in particular, also complex and not real.

scholarly journals BiteOscope: an open platform to study mosquito blood-feeding behavior

2020 ◽  
Author(s):  
Felix JH Hol ◽  
Louis Lambrechts ◽  
Manu Prakash
Keyword(s):  
Blood Meal ◽  
Human Subjects ◽  
Blood Feeding ◽  
Anopheles Coluzzii ◽  
Open Platform ◽  
Essential Nutrient ◽  
Resolution Imaging ◽  
The Common ◽  
New Perspective

AbstractFemale mosquitoes need a blood meal to reproduce, and in obtaining this essential nutrient they transmit deadly pathogens. Although crucial for the spread of mosquito-borne diseases, our understanding of skin exploration, probing, and engorgement, is limited due to a lack of quantitative tools. Indeed, studies often expose human subjects to assess biting behavior. Here, we present the biteOscope, a device that attracts mosquitoes to a host mimic which they bite to obtain an artificial blood meal. The host mimic is transparent, allowing high-resolution imaging of the feeding mosquito. Using machine learning we extract detailed behavioral statistics describing the locomotion, pose, biting, and feeding dynamics of Aedes aegypti, Aedes albopictus, Anopheles stephensi, and Anopheles coluzzii. In addition to characterizing behavioral patterns, we discover that the common insect repellent DEET repels Anopheles coluzzii upon contact with their legs. The biteOscope provides a new perspective on mosquito blood feeding, enabling high-throughput quantitative characterization of the effects physiological and environmental factors have on this lethal behavior.

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