Chromosome Organization and Cell Growth of Corynebacterium glutamicum

2020 ◽  
pp. 3-24
Author(s):  
Kati Böhm ◽  
Giacomo Giacomelli ◽  
Fabian Meyer ◽  
Marc Bramkamp
Keyword(s):  
Cell Growth ◽  
Corynebacterium Glutamicum ◽  
Chromosome Organization

Increased glucose utilization and cell growth of Corynebacterium glutamicum by modifying the glucose-specific phosphotransferase system (PTSGlc) genes

2016 ◽  
Vol 62 (12) ◽  
pp. 983-992 ◽  
Author(s):  
Jianzhong Xu ◽  
Junlan Zhang ◽  
Dongdong Liu ◽  
Weiguo Zhang
Keyword(s):  
Growth Rate ◽  
Cell Growth ◽  
Corynebacterium Glutamicum ◽  
Production Rate ◽  
Glucose Consumption ◽  
Chemical Mutagenesis ◽  
Transport Systems ◽  
Phosphotransferase System ◽  
Lysine Production ◽  
Consumption Growth

The phosphoenolpyruvate:glucose phosphotransferase system (PTSGlc) is the major pathway of glucose uptake in Corynebacterium glutamicum. This study investigated glucose consumption rate, cell growth, and metabolite changes resulting from modification of PTSGlc. The classical l-lysine producer C. glutamicum XQ-8 exhibited low glucose consumption, cell growth, and l-lysine production rates, whereas these parameters were significantly increased during cultivating on glucose plus maltose, through inactivation of SugR, or by overexpression of PTSGlc genes. XQ-8sugR::cat/pDXW-8-ptsI exhibited the highest increase in glucose consumption, growth rate, and l-lysine production, followed by XQ-8sugR::cat/pDXW-8-ptsG. However, overexpression of ptsH had little effect on the above-mentioned factors. Although co-overexpression of ptsGHI led to the highest glucose consumption, growth rate, and final l-lysine production; the l-lysine production rate was lower than that of XQ-8sugR::cat/pDXW-8-ptsIH. In fed-batch fermentation, XQ-8sugR::cat/pDXW-8-ptsIH had a higher growth rate of 0.54 h−1 to a dry cell mass of 66 g·L−1 after 16 h, and had a higher l-lysine production rate of 159.2 g·L−1 after 36 h. These results indicate that modification of the sugar transport systems improves amino acid production, especially for mutants obtained by repeated physical and (or) chemical mutagenesis. However, modification of these systems needs to be performed on a case-by-case basis.

scholarly journals Deletion of cgR_1596 and cgR_2070, Encoding NlpC/P60 Proteins, Causes a Defect in Cell Separation in Corynebacterium glutamicum R

2008 ◽  
Vol 190 (24) ◽  
pp. 8204-8214 ◽  
Author(s):  
Yota Tsuge ◽  
Hidetaka Ogino ◽  
Haruhiko Teramoto ◽  
Masayuki Inui ◽  
Hideaki Yukawa
Keyword(s):  
Cell Growth ◽  
Corynebacterium Glutamicum ◽  
Cell Separation ◽  
Morphological Changes ◽  
Genomic Region ◽  
Terminal Region ◽  
Growth Delay ◽  
Secretion Signal ◽  
Amino Terminal ◽  
Transmission Electron

ABSTRACT In previous work, random genome deletion mutants of Corynebacterium glutamicum R were generated using the insertion sequence (IS) element IS31831 and the Cre/loxP excision system. One of these mutants, C. glutamicum strain RD41, resulting from the deletion of a 10.1-kb genomic region (ΔcgR_1595 through cgR_1604) from the WT strain, showed cell elongation, and several lines appeared on the cell surface (bamboo shape). The morphological changes were suppressed by overexpression of cgR_1596. Single disruption of cgR_1596 in WT C. glutamicum R resulted in morphological changes similar to those observed in the RD41 strain. CgR_1596 has a predicted secretion signal peptide in the amino-terminal region and a predicted NlpC/P60 domain, which is conserved in cell wall hydrolases, in the carboxyl-terminal region. In C. glutamicum R, CgR_0802, CgR_1596, CgR_2069, and CgR_2070 have the NlpC/P60 domain; however, only simultaneous disruption of cgR_1596 and cgR_2070, and not cgR_2070 single disruption, resulted in cell growth delay and more severe morphological changes than disruption of cgR_1596. Transmission electron microscopy revealed multiple septa within individual cells of cgR_1596 single and cgR_1596-cgR_2070 double disruptants. Taken together, these results suggest that cgR_1596 and cgR_2070 are involved in cell separation and cell growth in C. glutamicum.

scholarly journals Single-cell growth inference of Corynebacterium glutamicum reveals asymptotically linear growth

2020 ◽  
Author(s):  
Joris Messelink ◽  
Fabian Meyer ◽  
Marc Bramkamp ◽  
Chase P. Broedersz
Keyword(s):  
Cell Growth ◽  
Single Cell ◽  
Corynebacterium Glutamicum ◽  
Cell Size ◽  
Linear Growth ◽  
Growth Dynamics ◽  
Asymptotically Linear ◽  
Inference Method ◽  
Growth Modes ◽  
Regulation Mechanisms

AbstractIn many bacteria, protein mass production is thought to be rate limiting for growth, implying exponential growth at the single cell level. To maintain cell-size homeostasis in proliferating populations of exponentially growing bacteria, tight growth and division mechanisms are required. However, it remains unclear whether these considerations set universal physical limits to bacterial growth. Here, we characterize the growth dynamics of the actinobacterium Corynebacterium glutamicum - a promising candidate for uncovering novel growth modes. This bacterium exhibits apical cell wall synthesis and division site selection systems appear to be absent, as reflected by a broad distribution of division asymmetries. We develop a novel growth inference method that averages out measurement noise and single-cell variability to obtain elongation rate curves as a function of birth length. Using this approach, we find that C. glutamicum exhibits asymptotically linear single-cell growth. To explain this growth mode, we model elongation as being rate-limited by the apical growth mechanism mediated by cell wall transglycosylases. This model accurately reproduces the observed elongation rate curves, and we further validate the model with growth measurements on a transglycosylase deficient ΔrodA mutant. Finally, with simulations we show that asymptotically linear growth yields a narrower distribution of cell lengths, suggesting that this growth mode can act as a substitute for tight division length and division symmetry regulation.SignificanceRegulation of growth and cell size is crucial for the optimization of bacterial cellular function. So far, single bacterial cells have been found to grow exponentially, which implies the need for tight regulation mechanisms to maintain cell size throughout growth and division cycles. Here, we characterize the growth behavior of the apically growing bacterium Corynebacterium glutamicum, by developing a novel and broadly applicable inference method for single-cell growth dynamics. We find that this bacterium grows asymptotically linearly, enabling it to maintain a narrow distribution of cell sizes, despite having a large variability of single-cell growth features. Our results imply a novel interplay between mode of growth and division regulation mechanisms, which may extend to other bacteria with non-exponential growth modes.

scholarly journals Single-cell growth inference of Corynebacterium glutamicum reveals asymptoticallylinear growth

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Joris Jan Boudewijn Messelink ◽  
Fabian Meyer ◽  
Marc Bramkamp ◽  
Chase P Broedersz
Keyword(s):  
Cell Growth ◽  
Single Cell ◽  
Corynebacterium Glutamicum ◽  
Cell Size ◽  
Linear Growth ◽  
Growth Dynamics ◽  
Growth Mode ◽  
Bacterial Cells ◽  
Asymptotically Linear ◽  
Regulation Of Growth

Regulation of growth and cell size is crucial for the optimization of bacterial cellular function. So far, single bacterial cells have been found to grow predominantly exponentially, which implies the need for tight regulation to maintain cell size homeostasis. Here, we characterize the growth behavior of the apically growing bacterium Corynebacterium glutamicum using a novel broadly applicable inference method for single-cell growth dynamics. Using this approach, we find that C. glutamicum exhibits asymptotically linear single-cell growth. To explain this growth mode, we model elongation as being rate-limited by the apical growth mechanism. Our model accurately reproduces the inferred cell growth dynamics and is validated with elongation measurements on a transglycosylase deficient ΔrodA mutant. Finally, with simulations we show that the distribution of cell lengths is narrower for linear than exponential growth, suggesting that this asymptotically linear growth mode can act as a substitute for tight division length and division symmetry regulation.

scholarly journals Chromosome Segregation Impacts on Cell Growth and Division Site Selection in Corynebacterium glutamicum

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e55078 ◽  
Author(s):  
Catriona Donovan ◽  
Astrid Schauss ◽  
Reinhard Krämer ◽  
Marc Bramkamp
Keyword(s):  
Cell Growth ◽  
Corynebacterium Glutamicum ◽  
Chromosome Segregation ◽  
Site Selection ◽  
Division Site

Single‐cell growth behavior of Corynebacterium glutamicum under pH oscillations

2020 ◽  
Vol 92 (9) ◽  
pp. 1238-1238
Author(s):  
S. Täuber ◽  
L. Blöbaum ◽  
A. Grünberger
Keyword(s):  
Cell Growth ◽  
Single Cell ◽  
Corynebacterium Glutamicum ◽  
Growth Behavior

Cell growth and cell division in the rod-shaped actinomycete Corynebacterium glutamicum

2008 ◽  
Vol 94 (1) ◽  
pp. 99-109 ◽  
Author(s):  
Michal Letek ◽  
María Fiuza ◽  
Efrén Ordóñez ◽  
Almudena F. Villadangos ◽  
Astrid Ramos ◽  
...  
Keyword(s):  
Cell Division ◽  
Cell Growth ◽  
Corynebacterium Glutamicum

scholarly journals A hybrid Embden–Meyerhof–Parnas pathway provides a synthetic link between sugar and phosphate metabolism

2020 ◽  
Author(s):  
Yoojin Lee ◽  
Hye Jeong Cho ◽  
Han Min Woo
Keyword(s):  
Energy Metabolism ◽  
Cell Growth ◽  
Corynebacterium Glutamicum ◽  
Inorganic Phosphate ◽  
Kinase Activity ◽  
Phosphorus Metabolism ◽  
Phosphate Metabolism ◽  
Trehalose Biosynthesis ◽  
Biochemical Production ◽  
Glucose Kinase

AbstractThe fundamental Embden–Meyerhoff–Paranas (EMP) pathway for sugar catabolism, anabolism, and energy metabolism has been reconstituted with non-oxidative glycolysis (NOG). Although carbon conservation was achieved via NOG, the energy metabolism was significantly limited. Herein, we showed the construction of a hybrid EMP that replaced the first phase of the EMP in Corynebacterium glutamicum with NOG and revealed a metabolic link of carbon and phosphorus metabolism. In accordance with synthetic glucose kinase activity and phosphoketolase on the hybrid EMP, cell growth was completely recovered in the C. glutamicum pfkA mutant strain where the first phase of EMP was eliminated. Notably, we have revealed a phosphate-replenishing pathway that involved trehalose biosynthesis for the generation of inorganic phosphate (Pi) sources in the hybrid EMP when external Pi supply was limited. Thus, the re-designed hybrid EMP pathway with balanced carbon and phosphorus states provides an efficient microbial platform for biochemical production.

scholarly journals Chromosome organization by a conserved condensin-ParB system in the actinobacterium Corynebacterium glutamicum

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Kati Böhm ◽  
Giacomo Giacomelli ◽  
Andreas Schmidt ◽  
Axel Imhof ◽  
Romain Koszul ◽  
...  
Keyword(s):  
Corynebacterium Glutamicum ◽  
Chromosome Organization
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