Analysis of the Potential Associated with the Siderophores Synthesis in the Bacillus Subtilis Strain Using Whole Genome Sequencing

ABSTRACTKnowledge of how microorganisms respond and adapt to low-pressure (LP) environments is limited. Previously,Bacillus subtilisstrain WN624 was grown at the near-inhibitory LP of 5 kPa for 1,000 generations and strain WN1106, which exhibited increased relative fitness at 5 kPa, was isolated. Genomic sequence differences between ancestral strain WN624 and LP-evolved strain WN1106 were identified using whole-genome sequencing. LP-evolved strain WN1106 carried amino acid-altering mutations in the coding sequences of only seven genes (fliI,parC,ytoI,bacD,resD,walK, andyvlD) and a single 9-nucleotide in-frame deletion in thernjBgene that encodes RNase J2, a component of the RNA degradosome. By using a collection of frozen stocks of the LP-evolved culture taken at 50-generation intervals, it was determined that (i) the fitness increase at LP occurred rapidly, while (ii) mutation acquisition exhibited complex kinetics. A knockout mutant ofrnjBwas shown to increase the competitive fitness ofB. subtilisat both LP and standard atmospheric pressure.

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Draft Genome Sequence of the Type Strain Bacillus subtilis subsp. subtilis DSM10

Microbiology Resource Announcements ◽

10.1128/mra.00158-21 ◽

2021 ◽

Vol 10 (10) ◽

Author(s):

Lars Lilge ◽

Robert Hertel ◽

Kambiz Morabbi Heravi ◽

Marius Henkel ◽

Fabian M. Commichau ◽

...

Keyword(s):

Bacillus Subtilis ◽

Whole Genome Sequencing ◽

Genome Sequencing ◽

Genome Sequence ◽

Type Strain ◽

Draft Genome ◽

Draft Genome Sequence ◽

Whole Genome ◽

Content Type ◽

Subtilis Subsp

ABSTRACT The Bacillus subtilis subsp. subtilis type strain DSM10 has been used as a reference in various studies. However, detailed information about the genome has not been available. Therefore, whole-genome sequencing was performed, and the sequence was compared with that of the related B. subtilis strain NCIB3610.

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Isolation, Whole-Genome Sequencing, and Annotation of Yimella sp. RIT 621, a Strain That Produces Antibiotic Compounds against Escherichia coli ATCC 25922 and Bacillus subtilis BGSC 168

Microbiology Resource Announcements ◽

10.1128/mra.00329-19 ◽

2019 ◽

Vol 8 (17) ◽

Author(s):

Anutthaman Parthasarathy ◽

Narayan H. Wong ◽

Nicolas D. Burns ◽

Atlantis H. Aziz-Dickerson ◽

Joyceline Dweh ◽

...

Keyword(s):

Escherichia Coli ◽

Bacillus Subtilis ◽

Whole Genome Sequencing ◽

Genome Sequencing ◽

Whole Genome ◽

Negative Bacterium ◽

Positive Bacterium ◽

Gram Negative ◽

Content Type ◽

Antibiotic Compounds

Here, we report the isolation, identification, whole-genome sequencing, and annotation of the bacterium Yimella sp. strain RIT 621. Concentrated spent medium extract treated with ethyl acetate was found to produce bactericidal compounds against the Gram-positive bacterium Bacillus subtilis BGSC 168 and the Gram-negative bacterium Escherichia coli ATCC 25922.

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