Biosorption of Copper by Saccharomyces cerevisiae: From Biomass Characterization to Process Development

2017 ◽  
pp. 205-224
Author(s):  
Pietro Altimari ◽  
Fabrizio Di Caprio ◽  
Francesca Pagnanelli
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Process Development ◽  
Biomass Characterization

Process development for the production of recombinant hirudin in Saccharomyces cerevisiae: from upstream to downstream

1995 ◽  
Vol 30 (7) ◽  
pp. 653-660 ◽  
Author(s):  
J.-H. Sohn ◽  
E.-S. Choi ◽  
B.-H. Chung ◽  
D.-J. Youn ◽  
J.-H. Seo ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Process Development ◽  
Recombinant Hirudin

scholarly journals Accelerated Bioprocess Development of Endopolygalacturonase-Production with Saccharomyces cerevisiae Using Multivariate Prediction in a 48 Mini-Bioreactor Automated Platform

2018 ◽  
Vol 5 (4) ◽  
pp. 101 ◽  
Author(s):  
Annina Sawatzki ◽  
Sebastian Hans ◽  
Harini Narayanan ◽  
Benjamin Haby ◽  
Niels Krausch ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Process Development ◽  
Principal Component ◽  
Product Formation ◽  
Screening Tools ◽  
Abnormal Behavior ◽  
Bioprocess Development ◽  
Online Data ◽  
Experimental Conditions ◽  
Promising Alternative

Mini-bioreactor systems enabling automatized operation of numerous parallel cultivations are a promising alternative to accelerate and optimize bioprocess development allowing for sophisticated cultivation experiments in high throughput. These include fed-batch and continuous cultivations with multiple options of process control and sample analysis which deliver valuable screening tools for industrial production. However, the model-based methods needed to operate these robotic facilities efficiently considering the complexity of biological processes are missing. We present an automated experiment facility that integrates online data handling, visualization and treatment using multivariate analysis approaches to design and operate dynamical experimental campaigns in up to 48 mini-bioreactors (8–12 mL) in parallel. In this study, the characterization of Saccharomyces cerevisiae AH22 secreting recombinant endopolygalacturonase is performed, running and comparing 16 experimental conditions in triplicate. Data-driven multivariate methods were developed to allow for fast, automated decision making as well as online predictive data analysis regarding endopolygalacturonase production. Using dynamic process information, a cultivation with abnormal behavior could be detected by principal component analysis as well as two clusters of similarly behaving cultivations, later classified according to the feeding rate. By decision tree analysis, cultivation conditions leading to an optimal recombinant product formation could be identified automatically. The developed method is easily adaptable to different strains and cultivation strategies, and suitable for automatized process development reducing the experimental times and costs.

Process development for high-level secretory production of carboxypeptidase Y by Saccharomyces cerevisiae

1998 ◽  
Vol 50 (1) ◽  
pp. 34-41 ◽  
Author(s):  
Y. Shiba ◽  
F. Fukui ◽  
K. Ichikawa ◽  
N. Serizawa ◽  
H. Yoshikawa
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Process Development ◽  
Carboxypeptidase Y ◽  
Secretory Production ◽  
High Level

Advanced TEM sample preparation techniques for submicron Si devices

1995 ◽  
Vol 53 ◽  
pp. 516-517 ◽  
Author(s):  
P. B. Basham ◽  
H. L. Tsai
Keyword(s):  
Sample Preparation ◽  
Process Development ◽  
Light Transmission ◽  
Specific Area ◽  
Turnaround Time ◽  
Small Hole ◽  
Area Of Interest ◽  
Transmission Electron ◽  
Polished Side ◽  
Preparation Techniques

The use of transmission electron microscopy (TEM) to support process development of advanced microelectronic devices is often challenged by a large amount of samples submitted from wafer fabrication areas and specific-spot analysis. Improving the TEM sample preparation techniques for a fast turnaround time is critical in order to provide a timely support for customers and improve the utilization of TEM. For the specific-area sample preparation, a technique which can be easily prepared with the least amount of effort is preferred. For these reasons, we have developed several techniques which have greatly facilitated the TEM sample preparation.For specific-area analysis, the use of a copper grid with a small hole is found to be very useful. With this small-hole grid technique, TEM sample preparation can be proceeded by well-established conventional methods. The sample is first polished to the area of interest, which is then carefully positioned inside the hole. This polished side is placed against the grid by epoxy Fig. 1 is an optical image of a TEM cross-section after dimpling to light transmission.

The Structure and Properties of MoSi2 Thin Film in Mos Process

1980 ◽  
Vol 38 ◽  
pp. 326-327
Author(s):  
C.K. Wu ◽  
P. Chang ◽  
N. Godinho
Keyword(s):  
Thin Film ◽  
Integrated Circuits ◽  
High Performance ◽  
Large Scale ◽  
Process Development ◽  
Structure And Properties ◽  
Metal Silicides ◽  
High Oxidation ◽  
Important Approach ◽  
High Oxidation Resistance

Recently, the use of refractory metal silicides as low resistivity, high temperature and high oxidation resistance gate materials in large scale integrated circuits (LSI) has become an important approach in advanced MOS process development (1). This research is a systematic study on the structure and properties of molybdenum silicide thin film and its applicability to high performance LSI fabrication.

Anti-Saccharomyces cerevisiae Antibodies in Inflammatory Bowel Disease: a Family Study

2001 ◽  
Vol 36 (2) ◽  
pp. 196-201 ◽  
Author(s):  
F. Seibold ◽  
O. Stich ◽  
R. Hufnagl ◽  
S. Kamil ◽  
M. Scheurlen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Family Study ◽  
Inflammatory Bowel
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