Uptake and Integration of Exogenous DNA in Plants

1989 ◽  
pp. 54-74 ◽  
Author(s):  
P. F. Lurquin
Keyword(s):  
Exogenous Dna

scholarly journals DNA repair and the evolution of transformation in Bacillus subtilis. II. Role of inducible repair.

Genetics ◽  
1989 ◽  
Vol 121 (3) ◽  
pp. 411-422
Author(s):  
M F Wojciechowski ◽  
M A Hoelzer ◽  
R E Michod
Keyword(s):  
Dna Repair ◽  
Bacillus Subtilis ◽  
Plasmid Dna ◽  
Excision Repair ◽  
Physiological State ◽  
Transformation Rate ◽  
Exogenous Dna ◽  
Expression Of Genes ◽  
Cell Subpopulations ◽  
Experimental Treatments

Abstract In Bacillus subtilis, DNA repair and recombination are intimately associated with competence, the physiological state in which the bacterium can bind, take up and recombine exogenous DNA. Previously, we have shown that the homologous DNA transformation rate (ratio of transformants to total cells) increases with increasing UV dosage if cells are transformed after exposure to UV radiation (UV-DNA), whereas the transformation rate decreases if cells are transformed before exposure to UV (DNA-UV). In this report, by using different DNA repair-deficient mutants, we show that the greater increase in transformation rate in UV-DNA experiments than in DNA-UV experiments does not depend upon excision repair or inducible SOS-like repair, although certain quantitative aspects of the response do depend upon these repair systems. We also show that there is no increase in the transformation rate in a UV-DNA experiment when repair and recombination proficient cells are transformed with nonhomologous plasmid DNA, although the results in a DNA-UV experiment are essentially unchanged by using plasmid DNA. We have used din operon fusions as a sensitive means of assaying for the expression of genes under the control of the SOS-like regulon in both competent and noncompetent cell subpopulations as a consequence of competence development and our subsequent experimental treatments. Results indicate that the SOS-like system is induced in both competent and noncompetent subpopulations in our treatments and so should not be a major factor in the differential response in transformation rate observed in UV-DNA and DNA-UV treatments. These results provide further support to the hypothesis that the evolutionary function of competence is to bring DNA into the cell for use as template in the repair of DNA damage.

scholarly journals Magnetic Nanoparticles Mediated-Gene Delivery for Simpler and More Effective Transformation of Pichia pastoris

2021 ◽  
Author(s):  
Seyda Yildiz ◽  
Kubra Solak ◽  
Melek Acar ◽  
Ahmet Mavi ◽  
Yagmur Unver
Keyword(s):  
Magnetic Nanoparticles ◽  
Pichia Pastoris ◽  
Gene Delivery ◽  
Delivery Method ◽  
Exogenous Dna ◽  
Novel Gene ◽  
A Cell

The introduction of exogenous DNA into a cell can be used to produce large quantities of protein. Here, we describe a novel gene delivery method into Pichia pastoris based on...

scholarly journals STR technique for the detection of contamination by exogenous DNA in paraffin blocks and histological slides

2019 ◽  
Vol 2 (1) ◽  
Author(s):  
Denise Barcelos ◽  
Karina Funabashi ◽  
Susana Mazloum ◽  
Mariana Fernandes ◽  
Leonardo Cardili
Keyword(s):  
Target Therapy ◽  
Genetic Material ◽  
Gastric Gist ◽  
Sequence Analyses ◽  
Laboratory Procedure ◽  
Exogenous Dna ◽  
Stromal Tumors ◽  
Specific Target ◽  
Different Types ◽  
Short Tandem

Abstract Gastrointestinal Stromal Tumors (GIST) present different types of mutations that may or may not be sensitive to specific target therapy. The laboratory procedure required to prepare histological sections traditionally demands multiple steps, making the process prone to contamination by exogenous genetic material (DNA). An eventual contamination of the biological sample with exogenous DNA may jeopardize subsequent analysis of mutations. The Short Tandem Repeat (STR) technique is frequently used in forensic science fields and presents a potential application in surgical pathology, especially in situations of suspected sample exchange. In the present study, the objective is to verify the possible contamination by exogenous DNA in gastric GIST samples and to evaluate if the presence of contamination can interfere in the detection of the mutations of interest. We assessed eight gastric GISTs by the Sanger sequencing and STR sequence analyses. Seven samples presented more than one profile, a result interpreted as contamination. Our results indicate that exogenous DNA contamination occurred in most of the samples studied and that this was more frequent in samples obtained from the slides than those obtained from the block. The presence of contamination did not inhibit the detection of the mutations of interest for a specific target therapy. Furthermore, the histologic block revealed to be more advantageously when compared to the slide for molecular pathology diagnosis.

scholarly journals Peptide-guided JC polyomavirus-like particles specifically target bladder cancer cells for gene therapy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Wei-Hong Lai ◽  
Chiung-Yao Fang ◽  
Ming-Chieh Chou ◽  
Mien-Chun Lin ◽  
Cheng-Huang Shen ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Bladder Cancer ◽  
Cancer Cells ◽  
Suicide Gene ◽  
Transduction Efficiency ◽  
Ligand Molecule ◽  
Jc Polyomavirus ◽  
Exogenous Dna ◽  
Bladder Cancer Cells

AbstractThe ultimate goal of gene delivery vectors is to establish specific and effective treatments for human diseases. We previously demonstrated that human JC polyomavirus (JCPyV) virus-like particles (VLPs) can package and deliver exogenous DNA into susceptible cells for gene expression. For tissue-specific targeting in this study, JCPyV VLPs were conjugated with a specific peptide for bladder cancer (SPB) that specifically binds to bladder cancer cells. The suicide gene thymidine kinase was packaged and delivered by SPB-conjugated VLPs (VLP-SPBs). Expression of the suicide gene was detected only in human bladder cancer cells and not in lung cancer or neuroblastoma cells susceptible to JCPyV VLP infection in vitro and in vivo, demonstrating the target specificity of VLP-SPBs. The gene transduction efficiency of VLP-SPBs was approximately 100 times greater than that of VLPs without the conjugated peptide. JCPyV VLPs can be specifically guided to target particular cell types when tagged with a ligand molecule that binds to a cell surface marker, thereby improving gene therapy.

Genetic modification of the waxy character in barley after an injection of wild-type exogenous DNA. Analysis of the second seed generation

1976 ◽  
Vol 36 (3) ◽  
pp. 303-310 ◽  
Author(s):  
V.N. Soyfer ◽  
N.A. Kartel ◽  
N.M. Chekalin ◽  
Y.B. Titov ◽  
K.K. Ciemenis ◽  
...  
Keyword(s):  
Genetic Modification ◽  
Dna Analysis ◽  
Wild Type ◽  
Exogenous Dna

Generation of biologically active retro-genes upon interaction of mouse spermatozoa with exogenous DNA

2006 ◽  
Vol 73 (10) ◽  
pp. 1239-1246 ◽  
Author(s):  
Carmine Pittoggi ◽  
Rosanna Beraldi ◽  
Ilaria Sciamanna ◽  
Laura Barberi ◽  
Roberto Giordano ◽  
...  
Keyword(s):  
Biologically Active ◽  
Exogenous Dna

scholarly journals Reduced Germline Mobility of a mariner Vector Containing Exogenous DNA: Effect of Size or Site?

Genetics ◽  
1996 ◽  
Vol 143 (3) ◽  
pp. 1299-1306 ◽  
Author(s):  
Allan R Lohe ◽  
Daniel L Hartl
Keyword(s):  
Genetic Engineering ◽  
Transposable Element ◽  
Frequency Analysis ◽  
Relative Stability ◽  
Low Frequency ◽  
Economic Importance ◽  
Exogenous Dna ◽  
Valuable Property ◽  
Reduced Mobility

Abstract Germline mobilization of the transposable element mariner is severely inhibited by the insertion of a 4.5- to 11.9-kb fragment of exogenous DNA into a unique SacI site approximately in the middle of the 1286bp element. In the presence of transposase driven by the germline-specific hsp26-sgs3 promoter, mobilization of the MlwB construct (containing a 11.9-kb insertion) is detected at low frequency. Analysis of a mobilized MlwB element indicated that mobilization is mediated by the marinertransposase. However, transposed MlwB elements are also defective in germline mobilization. Rare, transposase-induced germline excision events were also recovered for such vectors. The estimated rate of excision is <0.1% per chromosome per generation. Excision appears to be accompanied by gap repair if a suitable template is available. The data imply that the reduced mobility of mariner vectors with exogenous DNA in the SacI site results from disruption of sequences necessary for efficient mobilization. The relative stability may be a valuable property in the uses of mariner-like elements in genetic engineering of insects of economic importance.

Chromatid aberrations induced by exogenous DNA in Vicia faba

1977 ◽  
Vol 44 (2) ◽  
pp. 247-255 ◽  
Author(s):  
J. Šlotová ◽  
R. Rieger ◽  
I. Schubert ◽  
Z. Karpfel ◽  
A. Michaelis
Keyword(s):  
Vicia Faba ◽  
Exogenous Dna ◽  
Chromatid Aberrations

Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation

1991 ◽  
Vol 11 (4) ◽  
pp. 2328-2332
Author(s):  
L E Brown ◽  
S L Sprecher ◽  
L R Keller
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Plasmid Dna ◽  
Cell Walls ◽  
Intact Cell ◽  
Exogenous Dna ◽  
Electrical Pulses ◽  
Exogenous Plasmid

The fate of exogenous DNA introduced into Chlamydomonas reinhardtii by electroporation was analyzed. With single and double electrical pulses, plasmids as large as 14 kb were introduced into cells with and without intact cell walls. Within hours after introduction, exogenous plasmid DNA was associated with nuclei isolated from cells; several weeks after introduction, exogenous DNA was stably integrated into the Chlamydomonas genome. These studies establish electroporation as a method for introducing DNA, and potentially other molecules, into C. reinhardtii.

Sign in / Sign up

Export Citation Format

Share Document