In Vitro Cytocompatibility and Osteogenic Potential of Biodegradable Mg–Sr Alloys

Osteogenesis imperfecta (OI) is a genetic disease characterized by bone fragility and repeated fractures. The bone fragility associated with OI is caused by a defect in collagen formation due to mutation of COL1A1 or COL1A2. Current strategies for treating OI are not curative. In this study, we generated induced pluripotent stem cells (iPSCs) from OI patient-derived blood cells harboring a mutation in the COL1A1 gene. Osteoblast (OB) differentiated from OI-iPSCs showed abnormally decreased levels of type I collagen and osteogenic differentiation ability. Gene correction of the COL1A1 gene using CRISPR/Cas9 recovered the decreased type I collagen expression in OBs differentiated from OI-iPSCs. The osteogenic potential of OI-iPSCs was also recovered by the gene correction. This study suggests a new possibility of treatment and in vitro disease modeling using patient-derived iPSCs and gene editing with CRISPR/Cas9.

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Comparing the osteogenic potential of schneiderian membrane and dental pulp mesenchymal stem cells: an in vitro study

Cell and Tissue Banking ◽

10.1007/s10561-020-09887-4 ◽

2021 ◽

Author(s):

Antoine Berbéri ◽

Joseph Sabbagh ◽

Rita Bou Assaf ◽

Michella Ghassibe-Sabbagh ◽

Fatima Al-Nemer ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Dental Pulp ◽

In Vitro Study ◽

Vitro Study ◽

Osteogenic Potential ◽

Schneiderian Membrane

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Synthesis and Characterization of Silver–Strontium (Ag-Sr)-Doped Mesoporous Bioactive Glass Nanoparticles

Gels ◽

10.3390/gels7020034 ◽

2021 ◽

Vol 7 (2) ◽

pp. 34

Author(s):

Shaher Bano ◽

Memoona Akhtar ◽

Muhammad Yasir ◽

Muhammad Salman Maqbool ◽

Akbar Niaz ◽

...

Keyword(s):

Bioactive Glass ◽

Composite Coatings ◽

Antibacterial Properties ◽

Bone Diseases ◽

Osteogenic Potential ◽

Diffusion Method ◽

Charge Composition ◽

In Vitro Bioactivity ◽

Mesoporous Bioactive Glass

Biomedical implants are the need of this era due to the increase in number of accidents and follow-up surgeries. Different types of bone diseases such as osteoarthritis, osteomalacia, bone cancer, etc., are increasing globally. Mesoporous bioactive glass nanoparticles (MBGNs) are used in biomedical devices due to their osteointegration and bioactive properties. In this study, silver (Ag)- and strontium (Sr)-doped mesoporous bioactive glass nanoparticles (Ag-Sr MBGNs) were prepared by a modified Stöber process. In this method, Ag+ and Sr2+ were co-substituted in pure MBGNs to harvest the antibacterial properties of Ag ions, as well as pro-osteogenic potential of Sr2 ions. The effect of the two-ion concentration on morphology, surface charge, composition, antibacterial ability, and in-vitro bioactivity was studied. Scanning electron microscopy (SEM), X-Ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) confirmed the doping of Sr and Ag in MBGNs. SEM and EDX analysis confirmed the spherical morphology and typical composition of MBGNs, respectively. The Ag-Sr MBGNs showed a strong antibacterial effect against Staphylococcus carnosus and Escherichia coli bacteria determined via turbidity and disc diffusion method. Moreover, the synthesized Ag-Sr MBGNs develop apatite-like crystals upon immersion in simulated body fluid (SBF), which suggested that the addition of Sr improved in vitro bioactivity. The Ag-Sr MBGNs synthesized in this study can be used for the preparation of scaffolds or as a filler material in the composite coatings for bone tissue engineering.

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Interrogating the Osteogenic Potential of Implant Surfaces In Vitro: A Review of Current Assays

Tissue Engineering Part B Reviews ◽

10.1089/ten.teb.2019.0312 ◽

2020 ◽

Vol 26 (3) ◽

pp. 217-229

Author(s):

Steven James Avery ◽

Wayne Nishio Ayre ◽

Alastair James Sloan ◽

Rachel Jane Waddington

Keyword(s):

Osteogenic Potential

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Osteogenic Potential of Autogenous Bone Grafts Harvested with Four Different Surgical Techniques

Journal of Dental Research ◽

10.1177/0022034511422718 ◽

2011 ◽

Vol 90 (12) ◽

pp. 1428-1433 ◽

Cited By ~ 61

Author(s):

R.J. Miron ◽

E. Hedbom ◽

N. Saulacic ◽

Y. Zhang ◽

A. Sculean ◽

...

Keyword(s):

Cell Attachment ◽

Surgical Techniques ◽

Bone Grafts ◽

Osteogenic Potential ◽

Autogenous Bone ◽

Mrna Levels ◽

Alizarin Red ◽

Osteogenic Response ◽

Autogenous Bone Grafts

The osteogenic potential of autogenous bone grafts is superior to that of allografts and xenografts because of their ability to release osteoinductive growth factors and provide a natural osteoconductive surface for cell attachment and growth. In this in vitro study, autogenous bone particles were harvested by four commonly used techniques and compared for their ability to promote an osteogenic response. Primary osteoblasts were isolated and seeded on autogenous bone grafts prepared from the mandibles of miniature pigs with a bone mill, piezo-surgery, bone scraper, and bone drill (bone slurry). The osteoblast cultures were compared for their ability to promote cell attachment, proliferation, and differentiation. After 4 and 8 hrs, significantly higher cell numbers were associated with bone mill and bone scraper samples compared with those acquired by bone slurry and piezo-surgery. Similar patterns were consistently observed up to 5 days. Furthermore, osteoblasts seeded on bone mill and scraper samples expressed significantly elevated mRNA levels of collagen, osteocalcin, and osterix at 3 and 14 days and produced more mineralized tissue as assessed by alizarin red staining. These results suggest that the larger bone graft particles produced by bone mill and bone scraper techniques have a higher osteogenic potential than bone slurry and piezo-surgery.

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Combination of Human Amniotic Fluid Derived-Mesenchymal Stem Cells and Nano-hydroxyapatite Scaffold Enhances Bone Regeneration

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2019.730 ◽

2019 ◽

Vol 7 (17) ◽

pp. 2739-2750 ◽

Cited By ~ 1

Author(s):

Eman E. A. Mohammed ◽

Hanan Beherei ◽

Mohamed El-Zawahry ◽

Abdel Razik Farrag ◽

Naglaa Kholoussi ◽

...

Keyword(s):

Osteogenic Differentiation ◽

Bone Healing ◽

Therapeutic Potential ◽

Osteogenic Potential ◽

3D Scaffold ◽

Human Amniotic Fluid ◽

Post Transplantation ◽

Defect Site ◽

Tibia Defect

BACKGROUND: Human amniotic fluid-derived stem cells (hAF-MSCs) have a high proliferative capacity and osteogenic differentiation potential in vitro. The combination of hAF-MSCs with three-dimensional (3D) scaffold has a promising therapeutic potential in bone tissue engineering and regenerative medicine. Selection of an appropriate scaffold material has a crucial role in a cell supporting and osteoinductivity to induce new bone formation in vivo. AIM: This study aimed to investigate and evaluate the osteogenic potential of the 2nd-trimester hAF-MSCs in combination with the 3D scaffold, 30% Nano-hydroxyapatite chitosan, as a therapeutic application for bone healing in the induced tibia defect in the rabbit. SUBJECT AND METHODS: hAF-MSCs proliferation and culture expansion was done in vitro, and osteogenic differentiation characterisation was performed by Alizarin Red staining after 14 & 28 days. Expression of the surface markers of hAF-MSCs was assessed using Flow Cytometer with the following fluorescein-labelled antibodies: CD34-PE, CD73-APC, CD90-FITC, and HLA-DR-FITC. Ten rabbits were used as an animal model with an induced defect in the tibia to evaluate the therapeutic potential of osteogenic differentiation of hAF-MSCs seeded on 3D scaffold, 30% Nano-hydroxyapatite chitosan. The osteogenic differentiated hAF-MSCs/scaffold composite system applied and fitted in the defect region and non-seeded scaffold was used as control. The histopathological investigation was performed at 2, 3, & 4 weak post-transplantation and scanning electron microscope (SEM) was assessed at 2 & 4 weeks post-transplantation to evaluate the bone healing potential in the rabbit tibia defect. RESULTS: Culture and expansion of 2nd-trimester hAF-MSCs presented high proliferative and osteogenic potential in vitro. Histopathological examination for the transplanted hAF-MSCs seeded on the 3D scaffold, 30% Nano-hydroxyapatite chitosan, demonstrated new bone formation in the defect site at 2 & 3 weeks post-transplantation as compared to the control (non-seeded scaffold). Interestingly, the scaffold accelerated the osteogenic differentiation of AF-MSCs and showed complete bone healing of the defect site as compared to the control (non-seeded scaffold) at 4 weeks post-transplantation. Furthermore, the SEM analysis confirmed these findings. CONCLUSION: The combination of the 2nd-trimester hAF-MSCs and 3D scaffold, 30% Nano-hydroxyapatite chitosan, have a therapeutic perspective for large bone defect and could be used effectively in bone tissue engineering and regenerative medicine.

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Generation of iPSCs from Jaw Periosteal Cells Using Self-Replicating RNA

International Journal of Molecular Sciences ◽

10.3390/ijms20071648 ◽

2019 ◽

Vol 20 (7) ◽

pp. 1648 ◽

Cited By ~ 4

Author(s):

Felix Umrath ◽

Heidrun Steinle ◽

Marbod Weber ◽

Hans-Peter Wendel ◽

Siegmar Reinert ◽

...

Keyword(s):

Stem Cells ◽

Fluorescent Protein ◽

Osteogenic Potential ◽

Safety Standards ◽

Stem Cell Source ◽

Periosteal Cells ◽

Induced Pluripotent ◽

Green Fluorescent ◽

First Time

Jaw periosteal cells (JPCs) represent a suitable stem cell source for bone tissue engineering (BTE) applications. However, challenges associated with limited cell numbers, stressful cell sorting, or the occurrence of cell senescence during in vitro passaging and the associated insufficient osteogenic potential in vitro of JPCs and other mesenchymal stem/stromal cells (MSCs) are main hurdles and still need to be solved. In this study, for the first time, induced pluripotent stem cells (iPSCs) were generated from human JPCs to open up a new source of stem cells for BTE. For this purpose, a non-integrating self-replicating RNA (srRNA) encoding reprogramming factors and green fluorescent protein (GFP) as a reporter was used to obtain JPC-iPSCs with a feeder- and xeno-free reprogramming protocol to meet the highest safety standards for future clinical applications. Furthermore, to analyze the potential of these iPSCs as a source of osteogenic progenitor cells, JPC-iPSCs were differentiated into iPSC-derived mesenchymal stem/stromal like cells (iMSCs) and further differentiated to the osteogenic lineage under xeno-free conditions. The produced iMSCs displayed MSC marker expression and morphology as well as strong mineralization during osteogenic differentiation.

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