Isolation and Characterization of Pharmacologically Active Tannins from Stem Bark of Syzygium samarangense

2020 ◽  
pp. 549-561
Author(s):  
P. Asha Bhanu ◽  
M. Mohan Krishna Reddy ◽  
N. Sadhana Reddy ◽  
Bhattiprolu Kesava Rao
Keyword(s):  
Stem Bark ◽  
Isolation And Characterization ◽  
Pharmacologically Active

scholarly journals Isolation and characterization of aristolactam alkaloids from the stem bark of Goniothalamus velutinus (Airy Shaw) and their biological activities

2018 ◽  
Vol 30 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Erum Iqbal ◽  
Linda B.L. Lim ◽  
Kamariah Abu Salim ◽  
Shaheen Faizi ◽  
Ayaz Ahmed ◽  
...  
Keyword(s):  
Biological Activities ◽  
Stem Bark ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Antimicrobial Compound from Stem Bark of Traditional Medicinal Plant Sonneratia apetala: Evaluation of their antimicrobial, Cytotoxic and Antioxidant Properties

2021 ◽  
Vol 38 (1) ◽  
pp. 1-5
Author(s):  
Md Arifur Rahman ◽  
Ahmed Abu Rus’d ◽  
Md Enamul Haque
Keyword(s):  
Brine Shrimp ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Chloroform Extract ◽  
Free Radical Scavenging ◽  
Gram Negative Bacteria ◽  
Isolation And Characterization

Sonneratia apetala (S. apetala) (Lythraceae) has been investigated for the isolation and characterization of antimicrobial compounds and evaluation of their biological activities. The chloroform extract of the stem bark and different partitionate of the chloroform extracts i.e. Petroleum ether soluble fraction (PESF), Ethyl acetate soluble fraction(EASF), Methanol soluble fraction(MSF) and aqueous soluble fractions (ASF) were subjected to different chromatographic techniques to isolate secondary metabolites. Successive chromatographic separation and purification yielded a total of two compounds identified and characterized as Taraxerone(1) and 5,8-dihydroxy- 6-methoxy-4,9-dioxo-1,3,4,9-tetrahydronaphthol[2,3-c]furan-1-yl acetate (2) by extensive proton NMR spectrum (1H-NMRspectrum) analysis. The different partitionate like PESF, EASF, MESF and ASF were subjected to screen their antimicrobial properties against some selected Gram positive and Gram negative bacteria and fungi, brine shrimp lethality and antioxidant activities. The maximum zone of inhibition of chloroform extract was found against Pseudomonas sp. (16mm). All fractions showed more activity against Gram negative bacteria then Gram positive bacteria. In the brine shrimp lethality bioassay, among all extracts, the petroleum ether and ethyl acetate soluble fraction showed significant lethality having the LC50 value of 7.72 μg/ml. The antioxidant activity was evaluated in terms of determination of free radical scavenging activity (DPPH assay). Among all the extracts of S. apetala the highest free radical scavenging activity showed by (Methanol soluble fraction) MESF with IC50 value 18.0 μg/ml. Bangladesh J Microbiol, Volume 38, Number 1, June 2021, pp 1-5

scholarly journals Isolation, characterization and biological properties of betulin from Entada africana Guill. and Perr. (Mimosaceae).

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
A. Kwaji ◽  
H. M. Adamu ◽  
I. Y. Chindo ◽  
R. Atiko
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Biological Properties ◽  
Pure Compound ◽  
Isolation And Characterization ◽  
Isolated Compound

The present study is aimed at the isolation and characterization of betulin from Entada africana.  A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.

scholarly journals Isolation and Characterization of Secondary Metabilites and Evaluation of Antimicrobial, Antioxidant and Thrombolytic Potentials of Erythrina variegata L. Bark

2019 ◽  
Vol 31 (8) ◽  
pp. 1842-1846
Author(s):  
Fatema Tabassum ◽  
Choudhury Mahmood Hasan ◽  
Mohammad Mehedi Masud ◽  
Md. Imran Nur Manik ◽  
Monira Ahsan
Keyword(s):  
Methanolic Extract ◽  
Authentic Sample ◽  
Stem Bark ◽  
Moderate Activity ◽  
Zone Of Inhibition ◽  
Nmr Studies ◽  
Erythrina Variegata ◽  
Isolation And Characterization ◽  
Ic50 Value

Six compounds, sitosta-4-en-3-one (1), 3β,28-dihydroxyolean-12-en (2), scandenone (3), alpinum isoflavone (4), stigmasterol (5) and lupeol (6) were isolated from the methanol soluble extract of the stem bark of Erythrina variegata. The structure of the compounds was established by extensive NMR studies as well as co-TLC with authentic sample. The petroleum ether, carbon tetrachloride, chloroform and aqueous soluble fractions of the methanolic extract of Erythrina variegata were evaluated for antimicrobial, antioxidant and thrombolytic properties. In the antimicrobial study, most of the fractions of the extract exhibited mild to moderate antimicrobial activity where the zone of inhibition were ranging from 7.69 to 19.51 mm. The chloroform soluble fraction showed significant antioxidant activity with IC50 value 66.28 μg/mL as compared to standard BHT (IC50 value 23.09 μg/mL). The thrombolytic property of different extracts of E. variegata exhibited moderate activity ranging from 31.25 to 57.78 %.

scholarly journals A Novel Iridoid from Plumeria Obtusa

2008 ◽  
Vol 3 (2) ◽  
pp. 1934578X0800300
Author(s):  
Firdous Imran Ali ◽  
Imran Ali Hashmi ◽  
Bina Shaheen Siddiqui
Keyword(s):  
Natural Product ◽  
Stem Bark ◽  
Spectroscopic Studies ◽  
Chemical Transformations ◽  
Compound Identification ◽  
Synthetic Compound ◽  
First Report ◽  
Isolation And Characterization

Phytochemical investigations of fresh leaves and stem bark of Plumeria obtusa Linn. have led to the isolation and characterization of a new iridoid, p-methoxy- E-cinnamoyloxy plumieride (1), to which the trivial name champalinin has been assigned, and a triterpene lactone, 3β-hydroxy-urs-12-one-13β,28-olide (2). This is the first report of compound 2 as a natural product, although it has been reported earlier as a synthetic compound. Identification of these compounds was achieved through spectroscopic studies and chemical transformations.

scholarly journals Isolation and Characterization of Sesquiterpenes from Stem Bark of Warburgia ugandensis Sprague

2020 ◽  
Vol 9 (1) ◽  
pp. 39-46
Author(s):  
Teshome Gonfa Hordofa
Keyword(s):  
Medicinal Plants ◽  
Plant Species ◽  
Bioactive Compounds ◽  
Common Cold ◽  
Stem Bark ◽  
Spectroscopic Methods ◽  
Insufficient Information ◽  
Isolation And Characterization ◽  
Reported Data

Warburgia ugandensis Sprague is one of the medicinal plants traditionally used to treat a number of diseases like asthma, cough, diarrhea, common cold, stomachache and toothache in Ethiopia. However, there is still insufficient information on the isolation and evaluation of bioactive compounds from this plant species. Extraction, purification and isolation of the stem bark of this plant by dichloromethane and chloroform as solvents afforded two sesquiterpenes; namely, WU-1 (ugandensidial) and WU-2 (cinnamolide-3β-acetate) respectively. The structural elucidations of these bioactive compounds were accomplished by using a variety of spectroscopic methods (IR, UV and NMR). The spectroscopic results compared with the reported data in the literature.

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