Research-Based Biofunctional Aspects of Milk Protein-Derived Bioactive Peptides

2020 ◽  
pp. 133-159
Author(s):  
Suvartan Ranvir ◽  
Nancy Awasti ◽  
Pranali Nikam ◽  
Neelima Sharma
Keyword(s):  
Milk Protein ◽  
Bioactive Peptides

Effects of abomasal infusions of sodium caseinate, a hydrolysate of casein or a corresponding mixture of free amino acids on milk yield and composition in dairy cows

1995 ◽  
Vol 62 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Jai-Jun Choung ◽  
David G. Chamberlain
Keyword(s):  
Amino Acids ◽  
Dairy Cows ◽  
Free Amino Acids ◽  
Free Amino ◽  
Milk Protein ◽  
Bioactive Peptides ◽  
Basal Diet ◽  
Sodium Caseinate ◽  
Amino Acid Residues ◽  
Kinetics Of

SummaryThe effects of the form in which amino acids are presented to the abomasum on the milk production of dairy cows receiving a basal diet of grass silage and a barley-based supplement were examined in two experiments. Effects of abomasal infusions of sodium caseinate were compared with the effects of corresponding levels of either an enzymic hydrolysate of casein (Expt 1) or a corresponding mixture of free amino acids (FAA; Expt 2). In Expt 1, although the yield of protein in milk increased progressively with each level of infusion, the yields of protein were greater for the caseinate than for the hydrolysate. Again, in Expt 2, for milk protein yield, sodium caseinate was superior to FAA at the lower level of infusion. In both experiments, the hydrolysate and FAA treatments were associated with higher concentrations of fat in the milk. There were indications of differences in the pattern of secretion of glucagon between the caseinate and FAA treatments. It is concluded that the differences between treatments relate either to the kinetics of absorption of amino acid residues or to the action of bioactive peptides released during digestion of casein.

Postprandial Effects of a Proprietary Milk Protein Hydrolysate Containing Bioactive Peptides in Prediabetic Subjects: A Recent Study

2021 ◽  
pp. 129-146
Author(s):  
Tina Sartorius ◽  
Andrea Weidner ◽  
Tanita Dharsono ◽  
Audrey Boulier ◽  
Manfred Wilhelm ◽  
...  
Keyword(s):  
Milk Protein ◽  
Bioactive Peptides ◽  
Protein Hydrolysate

scholarly journals Sequential release of milk protein–derived bioactive peptides in the jejunum in healthy humans

2013 ◽  
Vol 97 (6) ◽  
pp. 1314-1323 ◽  
Author(s):  
Rachel Boutrou ◽  
Claire Gaudichon ◽  
Didier Dupont ◽  
Julien Jardin ◽  
Gheorghe Airinei ◽  
...  
Keyword(s):  
Milk Protein ◽  
Bioactive Peptides ◽  
Healthy Humans ◽  
Sequential Release

scholarly journals ACE Inhibitory and Antioxidative Bioactive Peptides Derived from Hydrolyzed Soy Milk

Molekul ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 56
Author(s):  
Sandra Hermanto ◽  
Annisa Septiana ◽  
Deni K Putera ◽  
Fitriah Hatiningsih ◽  
Anna Muawanah
Keyword(s):  
Milk Protein ◽  
Bioactive Peptides ◽  
Antioxidative Activity ◽  
Positive Impact ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Antihypertensive Activity ◽  
Degree Of Hydrolysis ◽  
Soy Milk ◽  
Dpph Method

Soy milk is a soybean processed product rich in protein as well as sources of bioactive peptides. Bioactive peptides defined as specific protein fragments that have a positive impact on body functions and conditions and may ultimately influence health. This study was conducted to explore the potential of hydrolyzed soy milk as a source of antioxidative and antihypertensive bioactive peptides through enzymatic hydrolysis. The initial treatment of soy milk protein was acidic precipitation with hydrochloric acid. Furthermore, protein precipitate was hydrolyzed using pepsin proteolytic enzyme with an enzyme: substrate ratio (1:5, 1:10 and 1:20). Protein hydrolysis was carried out for 0–48 hours at 37 °C in an acetate buffer pH 4.5. The soy milk protein hydrolysates were subjected to determination of % DH (Degree of Hydrolysis) and protein profile by SDS-PAGE (sodium dodecyl sulphate polyacrylamide gel electrophoresis). The antihypertensive assay was carried out by in vitro inhibition of the ACE enzyme (Angiotensin Converting Enzyme) and antioxidative activity assay using the DPPH method. The results showed that the optimum conditions for hydrolysis of soy milk were obtained at 40 hours with a % DH value of 53.24% in enzyme ratio 1:20 and the highest antihypertensive activity was obtained from 48 hours hydrolysis with % ACE inhibition value of 79.31%. The highest antioxidative activity of bioactive peptide was obtained at hydrolysis 48 hours with IC50 69.1034 µg / ml. After fractionated and LCMS characterized it was obtained 2 bioactive peptides with molecular weights of 8.954 and 2,696 kDa. These bioactive peptides from hydrolyzed of soymilk might be potential as an antihypertensive agent and reduce oxidative stress.

Milk Protein Hydrolysates and Bioactive Peptides

2016 ◽  
pp. 417-482 ◽  
Author(s):  
A. B. Nongonierma ◽  
M. B. O’Keeffe ◽  
R. J. FitzGerald
Keyword(s):  
Milk Protein ◽  
Bioactive Peptides ◽  
Protein Hydrolysates

Biofunctional properties of milk protein derived bioactive peptides -A review

2015 ◽  
Vol 34 (4) ◽  
Author(s):  
Prasad Patil ◽  
Akanksha Wadehra ◽  
Varsha Garg ◽  
Kanchan Munjal ◽  
Sudhir Kumar Tomar ◽  
...  
Keyword(s):  
Milk Protein ◽  
Bioactive Peptides ◽  
Therapeutic Potential ◽  
Proteolytic Enzymes ◽  
Biological Activities ◽  
Milk Proteins ◽  
Physiological Effect ◽  
Amino Acid Sequences ◽  
The Body ◽  
Biologically Active

Milk has long been acknowledged as a source of macro- and micro nutrients. Presently, several identified biologically active substances from milk and their derivatives has attracted much attention from the scientific community. These bioactive compounds confer many health benefits that might support disease prevention. Worldwide, there is an increasing interest in the therapeutic potential of bioactive peptides which collectively present a cornucopia of bioactivities for utilization in humans. Bioactive peptides are hydrolysates with specific amino acid sequences that exert a positive physiological effect on the body. Most of the biological activities are encrypted within the primary sequence of the native protein and can be released during digestion by proteolytic enzymes in the gastrointestinal tract or during fermentation and food processing. Milk protein is an important source of bioactive peptides which may contribute to regulate the nervous, gastrointestinal, and cardiovascular systems as well as the immune system. Milk protein derived bioactive peptides are shown to have antihypertensive, antimicrobial, immunomodulatory, antioxidative and mineral-binding properties. Bioactive peptides derived from milk proteins are of particular interest to the food industry due to the potential functional and physiological roles that they exhibit.

scholarly journals Identification and Detection of Bioactive Peptides in Milk and Dairy Products: Remarks about Agro-Foods

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3328 ◽  
Author(s):  
Himani Punia ◽  
Jayanti Tokas ◽  
Anurag Malik ◽  
Sonali Sangwan ◽  
Satpal Baloda ◽  
...  
Keyword(s):  
Dairy Products ◽  
Milk Protein ◽  
Bioactive Peptides ◽  
Imaging Techniques ◽  
Scientific Evidence ◽  
Milk Proteins ◽  
Beneficial Effects ◽  
Dairy Ingredients ◽  
Nutritional Benefits ◽  
Analytical Approaches

Food-based components represent major sources of functional bioactive compounds. Milk is a rich source of multiple bioactive peptides that not only help to fulfill consumers ‘nutritional requirements but also play a significant role in preventing several health disorders. Understanding the chemical composition of milk and its products is critical for producing consistent and high-quality dairy products and functional dairy ingredients. Over the last two decades, peptides have gained significant attention by scientific evidence for its beneficial health impacts besides their established nutrient value. Increasing awareness of essential milk proteins has facilitated the development of novel milk protein products that are progressively required for nutritional benefits. The need to better understand the beneficial effects of milk-protein derived peptides has, therefore, led to the development of analytical approaches for the isolation, separation and identification of bioactive peptides in complex dairy products. Continuous emphasis is on the biological function and nutritional characteristics of milk constituents using several powerful techniques, namely omics, model cell lines, gut microbiome analysis and imaging techniques. This review briefly describes the state-of-the-art approach of peptidomics and lipidomics profiling approaches for the identification and detection of milk-derived bioactive peptides while taking into account recent progress in their analysis and emphasizing the difficulty of analysis of these functional and endogenous peptides.

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