Use of isozyme patterns in characterization of potato mutant lines selected for agronomic quantitative traits

Euphytica ◽  
1991 ◽  
Vol 57 (3) ◽  
pp. 189-194
Author(s):  
H. Groza ◽  
R. Kahn
Keyword(s):  
Quantitative Traits ◽  
Isozyme Patterns ◽  
Mutant Lines

Morphological and molecular characterization of teosinte derived maize population

2020 ◽  
Vol 79 (04) ◽  
Author(s):  
Amarjeet Kumar ◽  
N. K. Singh ◽  
Sneha Adhikari ◽  
Anjali Joshi
Keyword(s):  
Molecular Characterization ◽  
Quantitative Traits ◽  
Morphological Characterization ◽  
Linkage Groups ◽  
Germplasm Enhancement ◽  
Maize Germplasm ◽  
Maize Population ◽  
Quantitative Characters ◽  
Morphological And Molecular Characterization

Germplasm enhancement seems to be an essential part of a breeding programme to improve resiliency, adaptability and productivity of the crops. To develop and diversify the maize germplasm, teosinte a wild relative of maize was integrated in crossing programme and BC1F4 lines were developed. Five BC1F4 lines along with teosinte and maize inbred DI-103 were characterised using quantitative characters and molecular markers. Morphological characterization was done with the help of visual parameters and quantitative traits and for molecular characterization fifty six SSR markers were used. SSR data were analysed with the help of software Mapmaker and twelve linkage groups were generated. Maximum allelic contributions from parent teosinte were found in the introgressed line AM-5 (53.4%) followed by AM-12 (48.9 %), whereas, least contribution of 34.1 % was found in AM-7. The maximum genetic distance among the introgressed lines was observed between AM-2 and AM-9 (0.75) followed by AM-2 and AM-7 (0.70), AM-7 and AM-9 (0.70). The maximum number of cob was found in AM-5 (5.00) followed by AM-2 (4.00). Grain yield per plant was found highest for AM-2 (100.00 g) followed by AM-12 (80.00 g), while, least value was observed for AM-7 (42.00 g). The results indicated differential parental contributions which leads to diversification in the progenies derived from diverse crosses in maize and further opined that such crosses seems to be essential for creating adapted germplasm to whom breeders are looking for.

scholarly journals Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Michael J Prigge ◽  
Matthieu Platre ◽  
Nikita Kadakia ◽  
Yi Zhang ◽  
Kathleen Greenham ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Early Embryo ◽  
Wild Type ◽  
The Family ◽  
Dependent Inhibition ◽  
Root Gravitropism ◽  
Specialized Function ◽  
Mutant Lines

The TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. The Arabidopsis genome encodes six TIR1/AFB proteins representing three of the four clades that were established prior to angiosperm radiation. To determine the role of these proteins in plant development we performed an extensive genetic analysis involving the generation and characterization of all possible multiply-mutant lines. We find that loss of all six TIR1/AFB proteins results in early embryo defects and eventually seed abortion, and yet a single wild-type allele of TIR1 or AFB2 is sufficient to support growth throughout development. Our analysis reveals extensive functional overlap between even the most distantly related TIR1/AFB genes except for AFB1. Surprisingly, AFB1 has a specialized function in rapid auxin-dependent inhibition of root growth and early phase of root gravitropism. This activity may be related to a difference in subcellular localization compared to the other members of the family.

scholarly journals Qualitative and Quantitative Characterization of Snake Gourd (Trichosanthes anguina L.) Mutant Lines

2019 ◽  
Vol 8 (08) ◽  
pp. 1797-1803
Author(s):  
Santanu Layek ◽  
M.K. Pandit ◽  
Sanket Kumar ◽  
Arindam Das ◽  
Ana mika ◽  
...  
Keyword(s):  
Quantitative Characterization ◽  
Qualitative And Quantitative ◽  
Mutant Lines

scholarly journals Characterization of Phosphoinositide-3-kinase, Class 3 (PIK3C3) Gene and Association Tests with Quantitative Traits in Pigs

2005 ◽  
Vol 18 (12) ◽  
pp. 1701-1707 ◽  
Author(s):  
J. H. Kim ◽  
B. H. Choi ◽  
H. T. Lim ◽  
E. W. Park ◽  
S. H. Lee ◽  
...  
Keyword(s):  
Quantitative Traits ◽  
Association Tests ◽  
Phosphoinositide 3 Kinase

Agromorphological Characterization of a Collection of Senna obtusifolia (L.) in Burkina Faso

2021 ◽  
pp. 26-35
Author(s):  
Haoua Nacambo ◽  
Kiswendsida Romaric Nanema ◽  
Boureima Sawadogo ◽  
Mahamadou Sawadogo
Keyword(s):  
Burkina Faso ◽  
Quantitative Traits ◽  
Breeding Program ◽  
Block Type ◽  
Agronomic Performance ◽  
Herbaceous Species ◽  
Senna Obtusifolia ◽  
Subsaharan Africa ◽  
Several Variables

Aims: Senna obtusifolia (L.) is an important wild herbaceous species for subsaharan Africa population. It is used in human food and traditional medicine to treat several deseases. In Burkina Faso, despite its potentialities, this species is of a little interest in scientific research. The aim of this study is to provide a good knowledge of the genetic diversity of this species. Study Design: The experimental design used was a randomized Fischer block type with three replications. Place and Duration of the Study: The study was carried out at Gampéla during 2018 rainy season. Methodology: Seventy (70) accessions of Senna obtusifolia were used for the evaluation of agromorphological variability. A total of twenty-seven (27) quantitative traits were studied. Results: The study revealed the existence of a great variability within the studied collection. Among the twenty-one (21) traits used for the analysis of variance (ANOVA), seventeen (17) variables significantly discriminate the accessions. A high heterogeneity was observed for the number of leaflets of the three first leaves and the number of floral pieces. In addition, significant correlations were observed between several variables. Indeed, the number of primary branches was positively correlated with the number of fruits per plant (r = 0.483), the number of grains per plant (r = 0.352) and the leaves biomass (r = 0.279). However, the number of days at flowering (NJF) is negatively correlated with the number of primary branches (r = -0.281). The variability obtained was structured in three groups. The third group is composed of accessions with the best agronomic performance that could be used in future breeding program. Conclusion: A high agromorphological diversity of Senna obtusifolia was observed and could be used in a breeding program.

scholarly journals The Arabidopsis ALDP protein homologue COMATOSE is instrumental in peroxisomal acetate metabolism

2007 ◽  
Vol 406 (3) ◽  
pp. 399-406 ◽  
Author(s):  
Mark A. Hooks ◽  
James E. Turner ◽  
Elaine C. Murphy ◽  
Katherine A. Johnston ◽  
Sally Burr ◽  
...  
Keyword(s):  
Glyoxylate Cycle ◽  
Sequence Length ◽  
Acetate Metabolism ◽  
Soluble Carbohydrate ◽  
Wild Type ◽  
Abc Protein ◽  
Atp Binding Cassette Transporter ◽  
Mutant Lines ◽  
Simple Sequence

The Arabidopsis acn (acetate non-utilizing) mutants were isolated by fluoroacetate-resistant germination and seedling establishment. We report the characterization of the acn2 mutant. Physiological analyses of acn2 showed that it possessed characteristics similar to those of the mutants cts (COMATOSE)-1 and pxa [peroxisomal ABC (ATP-binding-cassette) transporter]1. The acn2 locus was mapped to within 3 cM of the CTS gene on the bottom arm of chromosome IV using CAPS (cleavage amplification polymorphism) and SSLP (simple sequence-length polymorphism) markers. Crossing acn2 and cts-1 failed to restore the fluoroacetate-sensitive phenotype, suggesting that these mutations were allelic. Sequencing of the ACN2 locus revealed a C→T nonsense mutation in exon 13, which would have resulted in the elimination of the C-terminal hemitransporter domain of the encoded protein. Neither the full-length CTS protein nor the truncated protein was detected on immunoblots using either C-terminal- or N-terminal-specific anti-CTS antibodies respectively, demonstrating the absence of the entire CTS protein in acn2 mutants. Emerged seedlings of both cts-1 and pxa1 alleles displayed increased resistance to FAc (monofluoroacetic acid) compared with the corresponding wild-type seedlings. Complementation studies showed that mutation of the CTS gene was responsible for the FAc-resistant phenotype, as when the wild-type protein was expressed in both the cts-1 and pxa1 mutant lines, the strains became FAc-sensitive. Feeding studies confirmed that both acn2 and cts-1 mutants were compromised in their ability to convert radiolabelled acetate into soluble carbohydrate. These results demonstrate a role for the ABC protein CTS in providing acetate to the glyoxylate cycle in developing seedlings.

Characterization of cDNA clones for rye endosperm β-amylase and analysis of β-amylase deficiency in rye mutant lines

1991 ◽  
Vol 83 (2) ◽  
pp. 257-263 ◽  
Author(s):  
T. Rorat ◽  
J. Sadowski ◽  
F. Grellet ◽  
J. Daussant ◽  
M. Delseny
Keyword(s):  
Cdna Clones ◽  
Mutant Lines

scholarly journals Characterization of Quince (Cydonia) Cultivars Using Polyacrylamide Gel Electrophoresis

1988 ◽  
Vol 6 (2) ◽  
pp. 53-59 ◽  
Author(s):  
E.E. Sanchez ◽  
R.A. Menendez ◽  
L.S. Daley ◽  
R.B. Boone ◽  
O.L. Jahn ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Phenol Oxidase ◽  
Cydonia Oblonga ◽  
Genetic Characteristics ◽  
Isozyme Patterns ◽  
Gradient Electrophoresis ◽  
Apple Cultivars ◽  
Anionic Polyacrylamide

An investigation of available Cydonia (Cydonia oblonga Mill., quince, membrillo) germplasm by isozyme staining of anionic polyacrylamide gradient electrophoresis gels is described. The isozymes of acid phosphatase, esterase, peroxidase and phenol oxidase showed most diversity and usefulness for this purpose. Eleven groups of quince and two groups of x Pyronia (quince-pear crosses) were distinguished by their isozyme patterns. These patterns distinguish between groups of clonal accessions, and the patterns were constant for each accession during the test period (December, 1986 to August, 1987). Thus, these patterns were considered to represent genetic characteristics suitable for identification purposes. The diversity of isozyme patterns was much less than in Corylus and Pyrus populations previously sampled; and less than that of a restricted pool of apple cultivars previously examined.

scholarly journals Genetic evaluation and characterization of jute (Corchorus spp. L) genotypes using DUS

2014 ◽  
Vol 10 (2) ◽  
pp. 147-153 ◽  
Author(s):  
A Das ◽  
D Kumar
Keyword(s):  
Plant Height ◽  
Seed Weight ◽  
Quantitative Traits ◽  
Genetic Advance ◽  
High Heritability ◽  
Qualitative Characters ◽  
Fibre Fineness ◽  
Almost All ◽  
Varietal Characterization

In this study 32 jute (Corchorus spp.) varieties comprised of 18 olitorius and 14 capsularis were evaluated and characterized for five quantitative and 12 qualitative characters as per revised official DUS Test Guidelines of Jute. The experimental materials were grown in a RBD with three replications at Baarrackpore, India during 2009-10. Significant differences among genotypes noted almost for almost all the quantitative traits. PCV and GCV were highest for 1000 seed weight (33.78 and 33.29%) followed by fibre fineness (27.11 and 26.70%). Low variability was recorded in case of plant height. All the five characters exhibited more or less high heritability coupled with high genetic advance as per cent over mean. Out of 12 qualitative characters studied, in C. capsularis 8 traits were monomorphic, 4 traits were dimorphic. However, in C. olitorius no trait was monomorphic, 6 traits were dimorphic and 6 traits were polymorphic among varieties indicating their potential for varietal characterization. DOI: http://dx.doi.org/10.3329/sja.v10i2.18335 SAARC J. Agri., 10(2): 147-153 (2012)

scholarly journals Morphological characterization of mutant lines of Nagina22 in rice (Oryza sativa L.)

2019 ◽  
Vol 10 (2) ◽  
pp. 559
Author(s):  
N.P. Sakthi Avinash ◽  
K. Manonmani ◽  
R. Muthuvijayaragavan ◽  
S. Rajeswari ◽  
S. Manonmani ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Morphological Characterization ◽  
Mutant Lines
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