Granular sludge growth under different reactor liquid surface tensions in lab-scale upflow anaerobic sludge blanket reactors treating wastewater from sugar-beet processing

1995 ◽  
Vol 43 (6) ◽  
pp. 1122-1127 ◽  
Author(s):  
J. Thaveesri ◽  
B. Liessens ◽  
W. Verstraete
2004 ◽  
Vol 49 (11-12) ◽  
pp. 69-76 ◽  
Author(s):  
J.E. Schmidt ◽  
D.J. Batstone ◽  
I. Angelidaki

Upflow anaerobic sludge blanket reactors may offer a number of advantages over conventional mixed-tank, SBR, and biofilm reactors, including high space-loading, low footprint, and resistance to shocks and toxins. In this study, we assessed the use of upflow anaerobic sludge blanket (UASB) reactor technology as applied to anaerobic ammonia removal, or Anammox. Four 200 ml UASB reactors were inoculated with 50% (by volume) anaerobic granular sludge and 50% flocular sludge from different sources (all with the potential for containing Anammox organisms). Tools used to assess the reactors included basic analyses, fluorescent in-situ hybridisation, and mathematical modelling, with statistical non-linear parameter estimation. Two of the reactors showed statistically identical Anammox activity (i.e., identical kinetic parameters), with good ammonia and nitrite removal (0.14 kgNHx m-3 reactor day-1, with 99% ammonia removal). The third reactor also demonstrated significant Anammox activity, but with poor identifiability of parameters. The fourth reactor had no statistical Anammox activity. Modelling indicated that poor identifiability and performance in the third and fourth reactors were related to an excess of reduced carbon, probably originating in the inoculum. Accumulation of Anammox organisms was confirmed both by a volume loading much lower than the growth rate, and response to a probe specific for organisms previously reported to mediate Anammox processes. Overall, the UASB reactors were effective as Anammox systems, and identifiability of the systems was good, and repeatable (even compared to a previous study in a rotating biological contactor). This indicates that operation, design, and analysis of Anammox UASB reactors specifically, and Anammox systems in general, are reliable and portable, and that UASB systems are an appropriate technology for this process.


1997 ◽  
Vol 35 (10) ◽  
pp. 183-188 ◽  
Author(s):  
George R. Zoutberg ◽  
Peter de Been

In this paper a new type of anaerobic reactor is presented. The system has been developed by Biothane Systems and is marketed under the name Biobed® EGSB reactor (Expanded Granular Sludge Bed). In this reactor it is possible to grow and maintain a granular sludge under high liquid (10 m/h) and gas velocities (7 m/h). The most striking feature is the growth of biomass in a granular form, similar to the UASB granules: no carrier material is used. The process is specially suitable to treat waste water that contains compounds that are toxic in high concentrations and that only can be degraded in low concentrations (chemical industry). An example is given for a waste water originating from a chemical factory (Caldic Europoort) in the Netherlands. In this factory formaldehyde is produced from methanol. The waste water is characterised by high concentrations of these compounds (formaldehyde to 10 g/l and methanol to 20 g/l). Due to the special configuration of the anaerobic reactor it is possible to realise a removal efficiency for both compounds of more than 98%. It is also possible to operate the reactor as an ultra high loaded anaerobic reactor (to 30 kg COD/m3.day) for applications in other sectors of industry (e.g. brewery, yeast, sugar, corn ethanol production etc).


2013 ◽  
Vol 634-638 ◽  
pp. 182-186
Author(s):  
Juan Wang ◽  
Qin Zhong

With the aim to use anaerobic granular sludge, the methanogenic activity inhibition and recovery of anaerobic granular sludge from an industrial anaerobic reactor (s1) were investigated by measuring the methane volume at low pH. A lab-scale upflow anaerobic sludge blanket (UASB) reactor was inoculated with s1.s1 was used to remove Zn2+ in wastewater. The results show that activity of s1 is similar when the pH value is 6.5 to 7.0. The methane volume is obviously decreased when the pH value is 6.0. The activity is completely inhibited when the pH value is 4.5. The activity is fully recovered when the pH is above 6.5 and hardly recovers when the pH fell to 4.5. The main Zn2+ removal mechanism is chemical adsorption.


1989 ◽  
Vol 21 (4-5) ◽  
pp. 109-120 ◽  
Author(s):  
M. Yoda ◽  
M. Kitagawa ◽  
Y. Miyaji

The anaerobic expanded micro-carrier bed (MCB) process, which utilizes fine (50-100 microns) support materials as expanded bed media, was found to have the ability to cultivate granular sludge similar to that formed in the upflow anaerobic sludge blanket (UASB) process. Two laboratory-scale MCB reactors were studied with VFA and glucose wastewaters to clarify the role of the micro-carrier and the influence of substrates on granular sludge formation. Based on these results, a scale-up model with a reactor volume of 800 1 was successfully operated using molasses wastewater to demonstrate the feasibility of granular sludge formation in the MCB process.


1986 ◽  
Vol 18 (12) ◽  
pp. 99-108 ◽  
Author(s):  
Gatze Lettinga ◽  
Look Hulshoff Pol

Of the high rate anaerobic wastewater treatment systems the UASB (Upflow Anaerobic Sludge Blanket) reactor has found the widest application. Therefore the attention with respect to design, operation and economy will be focussed on this reactor type. In designing a UASB reactor specific attention is needed for the GSS (Gas-Solids Separator) device and the feed inlet system. For soluble wastewater generally no phase separation is required. Only for wastewaters high in suspended solids pre-acidification in a separate acidification reactor can be beneficial. Increasing attention is given to the development of modified UASB systems, such as a combination of a sludge bed reactor and an anaerobic filter. Other possible modified UASB systems may be found in a FS (Floating Settling) UASB reactor, the EGSB (Expanded Granular Sludge Bed) reactor and the UASB IC (Internal Circulation) reactor. As many factors are involved in the costs of a UASB reactor, only some rough data on reactor costs are presented.


2016 ◽  
Vol 74 (2) ◽  
pp. 500-507 ◽  
Author(s):  
Nguyen Thi Thanh ◽  
Takahiro Watari ◽  
Tran Phuong Thao ◽  
Masashi Hatamoto ◽  
Daisuke Tanikawa ◽  
...  

In this study, granular sludge formation was carried out using an aluminum chloride supplement in an upflow anaerobic sludge blanket (UASB) reactor treating natural rubber processing wastewater. Results show that during the first 75 days after the start-up of the UASB reactor with an organic loading rate (OLR) of 2.65 kg-COD·m−3·day−1, it performed stably with a removal of 90% of the total chemical oxygen demand (COD) and sludge still remained in small dispersed flocs. However, after aluminum chloride was added at a concentration of 300 mg·L−1 and the OLR range was increased up to 5.32 kg-COD·m−3·day−1, the total COD removal efficiency rose to 96.5 ± 2.6%, with a methane recovery rate of 84.9 ± 13.4%, and the flocs began to form granules. Massively parallel 16S rRNA gene sequencing of the sludge retained in the UASB reactor showed that total sequence reads of Methanosaeta sp. and Methanosarcina sp., reported to be the key organisms for granulation, increased after 311 days of operation. This indicates that the microbial community structure of the retained sludge in the UASB reactor at the end of the experiment gave a good account of itself in not only COD removal, but also granule formation.


1995 ◽  
Vol 31 (1) ◽  
pp. 249-259 ◽  
Author(s):  
Nina Christiansen ◽  
Hanne V. Hendriksen ◽  
Kimmo T. Järvinen ◽  
Birgitte K. Ahring

Data on anaerobic degradation of chloroaromatic compounds in Upflow Anaerobic Sludge Blanket Reactors (UASB-reactor) are presented and compared. Special attention is given to the metabolic pathways for degradation of chlorinated phenols by granular sludge. Results indicate that PCP can be degraded in UASB-reactors via stepwise dechlorination to phenol. Phenol will subsequently be converted to benzoate before ring cleavage. Dechlorination proceeds via different pathways dependent upon the inocula used. Results are further presented on the design of special metabolic pathways in granules which do not possess this activity using the dechlorinating organism, Desulfomonile tiedjei. Additionally, it is shown that it is possible to immobilize Dechlorosporium hafniense, a newly isolated dechlorinating anaerobe, into granular sludge, thereby introducing an ability not previously present in the granules.


1994 ◽  
Vol 30 (12) ◽  
pp. 43-53 ◽  
Author(s):  
J. Thaveesri ◽  
K. Gernaey ◽  
B. Kaonga ◽  
G. Boucneau ◽  
W. Verstraete

Laboratory studies of in-reactor granular-sludge yield (Ygran) were carried out to investigate the effect of substrates rich in proteins. Both lab-scale upflow anaerobic sludge blanket (UASB) and shake-flask systems were used to monitor the behaviour of the sludge. Influent based on molasses with a COD to N ratio of 100:2.5-3.0 gave good Ygran; increasing substitution of the carbohydrate COD by protein COD resulted in a deterioration of the sludge characteristics. The negative effects appear to be related to the protein rather than to the NH4+ formed. Of the various environmental parameters examined, the surface tension of the mixed liquor was the main deviating factor. At a proper range of oxygenation i.e., by means of monitoring of both the input COD to oxygen ratio (gCOD gO2−1) and the daily oxygen loading rate (mgO2 gVSS−1.d−1), the UASB reactors treating the nitrogen-rich wastewater showed enhanced Ygran with only a minor reduction in methanogenesis. A range of working conditions in which this principle can be applied to decrease fluffy growth to the advantage of granular increase is proposed.


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