Seasonal variation of trace metal concentrations in the digestive gland of the Mediterranean mussel Mytilus galloprovincialis: Comparison between a polluted and a non-polluted site

1994 ◽  
Vol 27 (1) ◽  
Author(s):  
F. Regoli ◽  
E. Orlando
Keyword(s):  
Seasonal Variation ◽  
Trace Metal ◽  
Digestive Gland ◽  
Mytilus Galloprovincialis ◽  
Polluted Site ◽  
Metal Concentrations ◽  
Mediterranean Mussel ◽  
The Mediterranean

Two novel multidrug resistance associated protein (MRP/ABCC) from the Mediterranean mussel (Mytilus galloprovincialis): characterization and expression patterns in detoxifying tissues

2015 ◽  
Vol 93 (7) ◽  
pp. 567-578 ◽  
Author(s):  
V. Lozano ◽  
R. Martínez-Escauriaza ◽  
M.L. Pérez-Parallé ◽  
A.J. Pazos ◽  
J.L. Sánchez
Keyword(s):  
Multidrug Resistance ◽  
Digestive Gland ◽  
Okadaic Acid ◽  
Abc Transporters ◽  
Mytilus Galloprovincialis ◽  
Expression Patterns ◽  
Cancer Resistance ◽  
Mediterranean Mussel ◽  
The Mediterranean

Multidrug resistance associated proteins (MRP) belong to the ABCC branch of the ABC transporters. The MRP together with P-gp (P-glycoprotein; MDR1; ABCB1) and BCRP (breast cancer resistance protein; ABCG2) confer multixenobiotic resistance (MXR) in marine vertebrates. In aquatic invertebrates, little is known about the presence and role of these ABC transporters. The ABC transporters play an important role in the absorption, distribution, and excretion of drugs, xenobiotics, and endogenous compounds and are predominantly expressed in excretory organs. In the present study, we identified and characterized two MRP/ABCC transporters (mrp1 and mrp2) from the Mediterranean mussel (Mytilus galloprovincialis Lamarck, 1819). The two cDNAs finally obtained were 4648 bp for mrp1 and 5065 bp for mrp2 with open reading frames of 1500 and 1524 residues, respectively. Analysis of the amino acid sequences revealed the structural organization of ABC transporters with the typical and highly conserved motifs. The expression levels of these genes revealed that the highest expression of mrp1 and mrp2 genes was found in the digestive gland followed by gills, and the lowest expression of the three tissues was detected in the mantle. The expression of these genes was also studied in mussels naturally contaminated with okadaic acid (from a bloom of Dinophysis acuminata Claparède and Lachmann, 1859). The overexpression of mrp2 in the digestive gland suggests that this gene is involved in the process of detoxification of okadaic acid in M. galloprovincilais. These expression patterns agree with the suggested role of these genes in the protection against endogenous or exogenous compounds in aquatic organisms.

scholarly journals A Comparative Assessment of the Chronic Effects of Micro- and Nano-Plastics on the Physiology of the Mediterranean Mussel Mytilus galloprovincialis

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 649
Author(s):  
Marco Capolupo ◽  
Paola Valbonesi ◽  
Elena Fabbri
Keyword(s):  
Mytilus Galloprovincialis ◽  
Acetylcholinesterase Activity ◽  
Particle Sizes ◽  
Glutathione S Transferase ◽  
Phase Ii Metabolism ◽  
Chronic Effects ◽  
Mediterranean Mussel ◽  
Metabolism Enzyme ◽  
The Mediterranean

The ocean contamination caused by micro- and nano-sized plastics is a matter of increasing concern regarding their potential effects on marine organisms. This study compared the effects of a 21-day exposure to 1.5, 15, and 150 ng/L of polystyrene microplastics (PS-MP, 3-µm) and nanoplastics (PS-NP, 50-nm) on a suite of biomarkers measured in the Mediterranean mussel Mytilus galloprovincialis. Endpoints encompassed immunological/lysosomal responses, oxidative stress/detoxification parameters, and neurotoxicological markers. Compared to PS-MP, PS-NP induced higher effects on lysosomal parameters of general stress. Exposures to both particle sizes increased lipid peroxidation and catalase activity in gills; PS-NP elicited greater effects on the phase-II metabolism enzyme glutathione S-transferase and on lysozyme activity, while only PS-MP inhibited the hemocyte phagocytosis, suggesting a major role of PS particle size in modulating immunological/detoxification pathways. A decreased acetylcholinesterase activity was induced by PS-NP, indicating their potential to impair neurological functions in mussels. Biomarker data integration in the Mussel Expert System identified an overall greater health status alteration in mussels exposed to PS-NP compared to PS-MP. This study shows that increasing concentrations of nanoplastics may induce higher effects than microplastics on the mussel’s lysosomal, metabolic, and neurological functions, eventually resulting in a greater impact on their overall fitness.

scholarly journals Tissue-scale microbiota of the Mediterranean mussel (Mytilus galloprovincialis) and its relationship with the environment

2020 ◽  
Vol 717 ◽  
pp. 137209 ◽  
Author(s):  
Margherita Musella ◽  
Rasika Wathsala ◽  
Teresa Tavella ◽  
Simone Rampelli ◽  
Monica Barone ◽  
...  
Keyword(s):  
Mytilus Galloprovincialis ◽  
Mediterranean Mussel ◽  
The Mediterranean

Styrene impairs normal embryo development in the Mediterranean mussel (Mytilus galloprovincialis)

2018 ◽  
Vol 201 ◽  
pp. 58-65 ◽  
Author(s):  
Rajapaksha Haddokara Gedara Rasika Wathsala ◽  
Silvia Franzellitti ◽  
Morena Scaglione ◽  
Elena Fabbri
Keyword(s):  
Embryo Development ◽  
Mytilus Galloprovincialis ◽  
Normal Embryo ◽  
Mediterranean Mussel ◽  
The Mediterranean

Lipids that contain arsenic in the Mediterranean mussel, Mytilus galloprovincialis

2020 ◽  
Vol 17 (3) ◽  
pp. 289 ◽  
Author(s):  
Fabiana P. Freitas ◽  
Georg Raber ◽  
Kenneth B. Jensen ◽  
António J. A. Nogueira ◽  
Kevin A. Francesconi
Keyword(s):  
High Performance ◽  
Mytilus Galloprovincialis ◽  
Trophic Levels ◽  
Food Sources ◽  
Tissue Type ◽  
Unicellular Algae ◽  
Molecular Mass Spectrometry ◽  
Mediterranean Mussel ◽  
The Mediterranean ◽  
First Time

Environmental contextAlthough arsenic-containing lipids are widespread in marine environments, their origin remains unknown. We show that the arsenolipids in a filter-feeding bivalve mollusc closely match those found in marine food sources, including unicellular algae and bacteria. The results demonstrate the role of lower trophic levels in determining the forms of arsenic found in higher organisms. AbstractArsenic-containing lipids, arsenolipids, are widely found among marine organisms, but their origin and possible biochemical roles remain unknown This work describes the diversity and abundance of arsenolipids in the digestive gland and mantle of nine specimens of the Mediterranean mussel, Mytilus galloprovincialis. By using high performance liquid chromatography (HPLC) coupled to both elemental and high-resolution molecular mass spectrometry, we identified 36 arsenolipids including arsenic derivatives of fatty acids, hydrocarbons, sugar-phospholipids and sugar-phytol; 21 of these arsenolipids were identified for the first time and included a new group comprising ether-phospholipids. The arsenic compounds in the mussels show distinct profiles depending on the tissue type, which provide insight into the arsenolipid origin. The results suggest that the presence of some arsenolipids in the mussels is from direct uptake of the compounds, presumably from food, rather than biogenesis within the mussels.

Sign in / Sign up

Export Citation Format

Share Document