Selection for NaCl tolerance in cell cultures of Medicago sativa and recovery of plants from a NaCl-tolerant cell line

A cell line with immature blast cell morphology was isolated from HL-60 promyelocytic leukemia cell cultures and designated HL-T. This new cell type is biphenotypic, expressing terminal transferase (TdT) together with myelomonocytoid immunologic features. TdT enzymatic activity, undetectable in HL-60, was determined to be 140 to 180 units/10(8) HL-T cells by the dGTP-assay, approximately 20% of the activity found in lymphoblastoid cell lines. HL-T predominantly synthesize the known 58- kDa TdT-protein plus a minor 54/56-kDa doublet. The 58-kDa steady state form is nonglycosylated and is phosphorylated. Precursor antigens S3.13 and MY-10, absent on HL-60, are expressed by HL-T; however, the cells are negative for HLA-Dr. Southern blot analysis by hybridization with immunoglobulin heavy chain (JH) and T cell-receptor chain gene (T beta) probes shows JH to be in the germ-line configuration in both cell lines and the T beta gene to be in germ-line in HL-60 but to be rearranged in HL-T. Truncation of the gene encoding the granulocyte-macrophage-colony- stimulating factor (GM-CSF), as found in HL-60, is not observed in HL- T. HL-T are resistant to differentiation-induction by retinoic acid and 1,25-dihydroxyvitamin D3. Cytogenetically HL-T share with HL-60 a deletion of the short arm of chromosome 9 at breakpoint p13, an aberration frequently found in patients with T cell leukemia. In addition, HL-T display t(8;9)(p11;p24) and trisomy 20. Tetraploidy is observed in 80% of HL-T metaphases with aberrations identical to those in the diploid karyotype. Like HL-60, the new line shows some surface- antigenic-T cell characteristics. Despite an antigenic pattern most consistent with that of helper-inducer T cells (T4+, D44+/-, 4B4+, 2H4- , TQ1+/-), HL-T cells and their conditioned culture medium suppress antigen, mitogen, and mixed-leukocyte-culture-mediated lymphocyte proliferation.

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In Vitro Selection of a Salt-Tolerant Cell Line from PEA Calli Cultures

Current Issues in Plant Molecular and Cellular Biology - Current Plant Science and Biotechnology in Agriculture ◽

10.1007/978-94-011-0307-7_49 ◽

1995 ◽

pp. 353-358

Author(s):

E. Olmos ◽

A. Piqueras ◽

E. Hellin

Keyword(s):

Cell Line ◽

In Vitro Selection ◽

Salt Tolerant ◽

Calli Cultures ◽

Tolerant Cell ◽

Selection Of

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The Immunohistochemical Characterisation of an SV40-immortalised Human Corneal Epithelial Cell Line

Alternatives to Laboratory Animals ◽

10.1177/026119290303100407 ◽

2003 ◽

Vol 31 (4) ◽

pp. 409-417 ◽

Cited By ~ 7

Author(s):

Anne Huhtala ◽

Sami K. Nurmi ◽

Hanna Tähti ◽

Lotta Salminen ◽

Päivi Alajuuma ◽

...

Keyword(s):

Epithelial Cells ◽

Single Cell ◽

Cell Line ◽

Cell Cultures ◽

Corneal Epithelium ◽

Culture Medium ◽

Corneal Epithelial Cell ◽

Cell Type ◽

Corneal Epithelial ◽

Single Cell Type

Alternatives to the Draize rabbit eye irritation test are currently being investigated. Because of morphological and biochemical differences between the rabbit and the human eye, continuous human cell lines have been proposed for use in ocular toxicology studies. Single cell-type monolayer cultures in culture medium have been used extensively in ocular toxicology. In the present study, an SV40-immortalised human corneal epithelial (HCE) cell line was characterised immunohistochemically, by using 13 different monoclonal antibodies to cytokeratins (CKs), ranging from CK3 to CK20. The results from the monolayer HCE cell cultures were compared with those from the corneal epithelium of human corneal cryostat sections. Previous studies have shown that the morphology of the HCE cell is similar to that of primary cultured human corneal epithelial cells, and that the cells express the cornea-specific CK3. In the study reported here, we show that the cell line also expresses CKs 7, 8, 18 and 19. These CKs are typically expressed by simple epithelial cells, and are not found in the human cornea in vivo. Therefore, the monolayer HCE cell line grown in culture medium does not express the CK pattern that is typical of human corneal epithelium. This should be taken into consideration when using HCE cell cultures in similar single cell-type experiments for ocular toxicology.

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ChemInform Abstract: ALKALOID PRODUCTION IN CATHARANTHUS ROSEUS CELL CULTURES. VII. EFFECT OF PARAMETER CHANGES AND CATABOLISM STUDIES ON CELL LINE PRL NO. 953

Chemischer Informationsdienst ◽

10.1002/chin.198201318 ◽

1982 ◽

Vol 13 (1) ◽

Author(s):

J. P. KUTNEY ◽

L. S. L. CHOI ◽

P. KOLODZIEJCZYK ◽

S. K. SLEIGH ◽

K. L. STUART ◽

...

Keyword(s):

Cell Line ◽

Cell Cultures ◽

Catharanthus Roseus ◽

Alkaloid Production

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Doxorubicin selection for MDR1/P-glycoprotein reduces swelling-activated K+ and Cl- currents in MES-SA cells

AJP Cell Physiology ◽

10.1152/ajpcell.1996.270.4.c1029 ◽

1996 ◽

Vol 270 (4) ◽

pp. C1029-C1036 ◽

Cited By ~ 11

Author(s):

D. B. Luckie ◽

M. E. Krouse ◽

T. C. Law ◽

B. I. Sikic ◽

J. J. Wine

Keyword(s):

Cell Line ◽

Anion Channel ◽

Uterine Sarcoma ◽

Protein Levels ◽

P Glycoprotein ◽

Whole Cell Patch Clamp ◽

Cl Channels ◽

Line Selection ◽

Selection For ◽

K Currents

To test the hypothesis that P-glycoprotein enhances swelling currents through regulation of volume-sensitive Cl- channels [recently termed VSOAC (volume-sensitive osmolyte and anion channel)], a human uterine sarcoma cell line (MES-SA) and its doxorubicin-selected counterpart (Dx5) were studied. P-glycoprotein mRNA and protein levels were detected only in Dx5 cells. However, whole cell patch-clamp experiments showed that swollen Dx5 cells (n = 5) produced smaller VSOAC currents than MES-SA cells (n = 4; 106 +/- 26 pA/pF vs. 232 +/- 76 pA/pF at 90 mV). In radioisotopic efflux experiments, both swelling-activated 125I (Cl-) currents (n = 15) and 86Rb (K+) currents (n = 8) were found to be two-to fourfold smaller in the Dx5 (high P-glycoprotein) cells. Inhibitors of P-glycoprotein showed no specificity for the doxorubicin-selected cells (Dx5). Dideoxyforskolin (100 microM) blocked swelling-activated 125I efflux equally in both cell lines, whereas 100 microM verapamil had no effect. Thus, in this cell line, selection for P-glycoprotein expression is associated with reduced swelling currents. These findings suggest that P-glycoprotein expression does not directly facilitate VSOAC.

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SELECTION FOR SEED SET IN NONINBRED AND PARTLY INBRED POPULATIONS OF ALFALFA

Canadian Journal of Plant Science ◽

10.4141/cjps77-126 ◽

1977 ◽

Vol 57 (3) ◽

pp. 873-881 ◽

Cited By ~ 2

Author(s):

R. MICHAUD ◽

T. H. BUSBICE

Keyword(s):

Medicago Sativa ◽

Seed Set ◽

Exponential Model ◽

Seed Setting ◽

Medicago Sativa L ◽

Selection For ◽

Inbred Populations ◽

The Relationship ◽

Advanced Generation ◽

Coefficient Of Inbreeding

Alfalfa (Medicago sativa L.) is a highly heterozygous cross-pollinating species, and most breeding efforts have been conducted on noninbred populations. The purpose of this study was to determine whether greater breeding progress could be made by selecting within partly inbred populations rather than within noninbred ones. One hundred and twenty F1 (noninbred) and 120 S1 (partly inbred) plants that were issued from crossing and selfing four alfalfa clones were evaluated for self-fertility. The most self-fertile 10% of the plants from each family were selected in each population. The selected plants within each level of inbreeding were intercrossed to produce an advanced generation in which the effectiveness of the selection was evaluated. Selection increased both self- and cross-fertility in the advanced generation. Selection was more effective at the F1 level than at the S1 level. Fertility was reduced drastically by inbreeding. The average self-fertility of the S1’s was only about 7% of the cross-fertility of their parental clones. An exponential model was proposed to describe the relationship between seed setting and the coefficient of inbreeding in the developing zygote. This model explained 95% of the variation among 11 unselected populations having differing levels of inbreeding.

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