Alkaline phosphatase isozymes of serum and urine and urinary protein in young men before and after running 3 km

1994 ◽  
Vol 69 (4) ◽  
pp. 355-360 ◽  
Author(s):  
Toyoji Sato ◽  
Hideo Sugimoto ◽  
Wu Xiao Yan ◽  
Kazuo Endo ◽  
Masaharu Yamamoto
Keyword(s):  
Alkaline Phosphatase ◽  
Young Men ◽  
Urinary Protein ◽  
Before And After ◽  
Serum And Urine

Electron microscopic study of the membrane glycoproteins in the rat kidney after cisplatin treatment

1989 ◽  
Vol 47 ◽  
pp. 912-913
Author(s):  
S.K. Aggarwal
Keyword(s):  
Alkaline Phosphatase ◽  
Rat Kidney ◽  
Light And Electron Microscopy ◽  
Kidney Tissue ◽  
Membrane Glycoproteins ◽  
Electron Microscopic ◽  
Before And After ◽  
Interstrand Cross Links ◽  
Cross Links

The proposed primary mechanism of action of the anticancer drug cisplatin (Cis-DDP) is through its interaction with DNA, mostly through DNA intrastrand cross-links or DNA interstrand cross-links. DNA repair mechanisms can circumvent this arrest thus permitting replication and transcription to proceed. Various membrane transport enzymes have also been demonstrated to be effected by cisplatin. Glycoprotein alkaline phosphatase was looked at in the proximal tubule cells before and after cisplatin both in vivo and in vitro for its inactivation or its removal from the membrane using light and electron microscopy.Outbred male Swiss Webster (Crl: (WI) BR) rats weighing 150-250g were given ip injections of cisplatin (7mg/kg). Animals were killed on day 3 and day 5. Thick slices (20-50.um) of kidney tissue from treated and untreated animals were fixed in 1% buffered glutaraldehyde and 1% formaldehyde (0.05 M cacodylate buffer, pH 7.3) for 30 min at 4°C. Alkaline phosphatase activity and carbohydrates were demonstrated according to methods described earlier.

scholarly journals Enterotoxin Production, DNA Repair and Alkaline Phosphatase of Vibrio cholerae Before and After Animal Passage

Microbiology ◽  
1982 ◽  
Vol 128 (9) ◽  
pp. 1927-1932 ◽  
Author(s):  
N. K. Roy ◽  
G. Das ◽  
T. S. Balganesh ◽  
S. N. Dey ◽  
R. K. Ghosh ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Dna Repair ◽  
Vibrio Cholerae ◽  
Enterotoxin Production ◽  
Before And After

Screening for hypophosphatasia: does biochemistry lead the way?

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Corinna Melanie Held ◽  
Anic Guebelin ◽  
Andreas Krebs ◽  
Jörn Oliver Sass ◽  
Michael Wurm ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Replacement Therapy ◽  
Pediatric Population ◽  
Laboratory Data ◽  
Diagnostic Algorithm ◽  
Inorganic Pyrophosphate ◽  
Enzyme Replacement ◽  
Underlying Diseases ◽  
Data Screening ◽  
Serum And Urine

Abstract Objectives Patients with childhood hypophosphatasia (HPP) often have unspecific symptoms. It was our aim to identify patients with mild forms of HPP by laboratory data screening for decreased alkaline phosphatase (AP) within a pediatric population. Methods We conducted a retrospective hospital-based data screening for AP activity below the following limits: Girls: ≤12 years: <125 U/L; >12 years: <50 U/L Boys: ≤14 years: <125 U/L; >14 years: <70 U/L. Screening positive patients with otherwise unexplained hypophosphatasemia were invited for further diagnostics: Re-test of AP activity, pyridoxal 5′-phosphate (PLP) in hemolyzed whole blood, phosphoethanolamine (PEA) in serum and urine, and inorganic pyrophosphate in urine. Sequencing of the ALPL gene was performed in patients with clinical and/or laboratory abnormalities suspicious for HPP. Results We assessed a total of 14,913 samples of 6,731 patients and identified 393 screening-positive patients. The majority of patients were excluded due to known underlying diseases causing AP depression. Of the 30 patients who participated in the study, three had a decrease in AP activity in combination with an increase in PLP and PEA. A heterozygous ALPL mutation was detected in each of them: One patient with a short stature was diagnosed with childhood-HPP and started with enzyme replacement therapy. The remaining two are considered as mutation carriers without osseous manifestation of the disease. Conclusions A diagnostic algorithm based on decreased AP is able to identify patients with ALPL mutation after exclusion of the differential diagnoses of hypophosphatasemia and with additional evidence of increased AP substrates.

AMINO ACIDS IN THE BLOOD AND URINE OF NORMAL AND ARTHRITIC SUBJECTS BEFORE AND AFTER A GLYCINE LOAD GIVEN WITH AND WITHOUT ADRENOCORTICOTROPIN

1957 ◽  
Vol 35 (1) ◽  
pp. 1005-1016 ◽  
Author(s):  
J. B. Derrick ◽  
Audrey P. Hanley
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Chromatographic Analysis ◽  
Free Amino ◽  
Serum Levels ◽  
Essential Amino Acids ◽  
Single Amino Acid ◽  
Normal Individuals ◽  
Before And After ◽  
Serum And Urine

Observations have been made on the specific free amino acids (chromatographic analysis) and other nitrogenous constituents in the serum and urine of normal and arthritic men under controlled dietary conditions, before and after a glycine load and adrenocorticotropin administered separately and together.Differences in the metabolism of amino acids between normal individuals and arthritics, particularly of alanine, proline, glutamic acid, taurine, and possibly tyrosine (and/or tryptophan) and cystine, were apparent. The differences were largely confined to the non-essential amino acids. Concomitant increases seen in the serum levels and in the excretion of several amino acids, in response to a load of a single amino acid, indicate that the increases in excretion are more than a matter of competition for reabsorption in the kidney. A prerenal phenomenon appears to be involved, possibly interconversion of amino acids. This concept is supported by the evidence that the increases in the serum levels were restricted to the non-essential amino acids.

Effect of salicylates on elevation of body temperature during exercise

1962 ◽  
Vol 17 (2) ◽  
pp. 323-325 ◽  
Author(s):  
John A. Downey ◽  
Robert C. Darling
Keyword(s):  
Body Temperature ◽  
Acetylsalicylic Acid ◽  
Rectal Temperature ◽  
Temperature Response ◽  
Young Men ◽  
Work Load ◽  
Conclusive Evidence ◽  
Blood Levels ◽  
Total Proteins ◽  
Before And After

An attempt was made to determine the effect of orally ingested acetylsalicylic acid (ASA) in moderate amounts for varying periods of time, on temperature response of ten healthy young men walking on a power-driven treadmill with a work load of approximately 6 mets. Three experiments were conducted, ranging from one dose of 1.8–2.4 g ASA to 3.6–4.8 g/day in divided doses for 48 hr, and the same amount in divided doses for 5–7 days, the dose in the first experiment and the last dose in those following being given 1frac12–2 hr before exercise. Rectal temperature was measured at rest, during exercise, and for 20–45 min of recovery. Blood levels of salicylate were determined, hematocrits were recorded, and total proteins were determined; each subject was weighed before and after exercise. It was found that rise in body temperature was not modified by ASA in any of the various dosages, nor was significant hemodilution of the blood caused. Suggestive, but not conclusive, evidence emerged indicating that salicylates accelerate the early stages of the fall in body temperature after exercise. Submitted on October 5, 1961

Improved agarose electrophoretic method for separating alkaline phosphatase isoenzymes in serum.

1988 ◽  
Vol 34 (9) ◽  
pp. 1857-1862 ◽  
Author(s):  
V O Van Hoof ◽  
L G Lepoutre ◽  
M F Hoylaerts ◽  
R Chevigné ◽  
M E De Broe
Keyword(s):  
Alkaline Phosphatase ◽  
Monoclonal Antibodies ◽  
Polyclonal Antibody ◽  
High Molecular Mass ◽  
High Reproducibility ◽  
Neuraminidase Treatment ◽  
Electrophoretic Method ◽  
Electrophoretic Mobilities ◽  
Before And After ◽  
Alkaline Phosphatase Isoenzymes

Abstract A modified agarose electrophoretic system for the separation of alkaline phosphatase (ALP, EC 3.1.3.1) isoenzymes is described. Bone, liver, high-molecular-mass, and intestinal ALP are separated with high reproducibility. The sensitivity of the agarose system is superior to cellulose acetate in detecting high-Mr ALP. Correlation is good between bone ALP fractions scanned before and after treatment with neuraminidase. Immunoglobulin-bound ALPs, the ALP-lipoprotein-X complex, and the additional ALP fraction observed in transient hyperphosphatasemia in children are detected by their peculiar electrophoretic mobility in the proposed system. Approximately 25% of the samples contained an additional fraction of intestinal-type ALP, as evidenced by neuraminidase treatment and use of polyclonal and monoclonal antibodies. Because the electrophoretic mobilities of this "intestinal variant" and of some immunoglobulin-bound ALP fractions are identical to those of bone and intestinal ALP, respectively, treatment of the samples with a polyclonal antibody that reacts with intestinal ALP is advised.

AMINO ACIDS IN THE BLOOD AND URINE OF NORMAL AND ARTHRITIC SUBJECTS BEFORE AND AFTER A GLYCINE LOAD GIVEN WITH AND WITHOUT ADRENOCORTICOTROPIN

1957 ◽  
Vol 35 (11) ◽  
pp. 1005-1016 ◽  
Author(s):  
J. B. Derrick ◽  
Audrey P. Hanley
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Chromatographic Analysis ◽  
Free Amino ◽  
Serum Levels ◽  
Essential Amino Acids ◽  
Single Amino Acid ◽  
Normal Individuals ◽  
Before And After ◽  
Serum And Urine

Observations have been made on the specific free amino acids (chromatographic analysis) and other nitrogenous constituents in the serum and urine of normal and arthritic men under controlled dietary conditions, before and after a glycine load and adrenocorticotropin administered separately and together.Differences in the metabolism of amino acids between normal individuals and arthritics, particularly of alanine, proline, glutamic acid, taurine, and possibly tyrosine (and/or tryptophan) and cystine, were apparent. The differences were largely confined to the non-essential amino acids. Concomitant increases seen in the serum levels and in the excretion of several amino acids, in response to a load of a single amino acid, indicate that the increases in excretion are more than a matter of competition for reabsorption in the kidney. A prerenal phenomenon appears to be involved, possibly interconversion of amino acids. This concept is supported by the evidence that the increases in the serum levels were restricted to the non-essential amino acids.

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