Alkaline phosphatase and acid phosphatase levels in saliva and serum of patients with healthy periodontium, gingivitis, and periodontitis before and after scaling with root planing: A clinico-biochemical study

The proposed primary mechanism of action of the anticancer drug cisplatin (Cis-DDP) is through its interaction with DNA, mostly through DNA intrastrand cross-links or DNA interstrand cross-links. DNA repair mechanisms can circumvent this arrest thus permitting replication and transcription to proceed. Various membrane transport enzymes have also been demonstrated to be effected by cisplatin. Glycoprotein alkaline phosphatase was looked at in the proximal tubule cells before and after cisplatin both in vivo and in vitro for its inactivation or its removal from the membrane using light and electron microscopy.Outbred male Swiss Webster (Crl: (WI) BR) rats weighing 150-250g were given ip injections of cisplatin (7mg/kg). Animals were killed on day 3 and day 5. Thick slices (20-50.um) of kidney tissue from treated and untreated animals were fixed in 1% buffered glutaraldehyde and 1% formaldehyde (0.05 M cacodylate buffer, pH 7.3) for 30 min at 4°C. Alkaline phosphatase activity and carbohydrates were demonstrated according to methods described earlier.

Download Full-text

Effects of Kanwa on Rat Gastrointestinal Phosphatases

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2010.3.3.13 ◽

2010 ◽

Vol 3 (3) ◽

pp. 1147-1152

Author(s):

Jacob Bamaiyi ◽

Omajali ◽

Sanni Momoh

Keyword(s):

Alkaline Phosphatase ◽

Small Intestine ◽

Acid Phosphatase ◽

Sodium Carbonate ◽

Elevated Level ◽

Food Additive ◽

Alkaline Phosphatases ◽

Acid And Alkaline Phosphatases ◽

Diagnostic Indices ◽

High Level

This study investigates the effects of kanwa on rat gastrointestinal phosphatases. The rats were administered 7% w/v concentration of trona (Kanwa) orally for a period of two weeks in order to investigate how this compound is being used as food additive in some homes in Nigeria. The Kanwa used in this study was the handpicked variety obtained from sellers from Anyigba market in eastern part of Kogi State, Nigeria. Kanwa, a hydrated sodium carbonate (Na2CO3NaHCO3.2H2O) was obtained as a dried lake salt. Acid phosphatase has the ability to dephosphorylate molecules containing phosphate group. The decreased and elevated level in serum or plasma acid and alkaline phosphatases serves as diagnostic indices for various diseases. Results showed that there was increase and decrease of acid phosphatase (ACP) activities in both the stomach and small intestine. The activities of alkaline phosphatase (ALP) fluctuated in the small intestine. However, in the stomach, an increase activity of ALP was noticed throughout the period of ‘Kanwa’ administration. We concluded that although the level of ‘Kanwa’ consumed in most homes may not be toxic if not taken continuously or repeatedly. Thus, continuous consumption should be discouraged as accumulation of high level of ‘Kanwa’ may cause damages or injuries to the various organs/tissues and may disrupt normal body function.

Download Full-text

Bone-Derived Serum Enzymes and Bone Density in Perimenopausal Caucasian Women

Annals of Clinical Biochemistry International Journal of Laboratory Medicine ◽

10.1177/000456329303000214 ◽

1993 ◽

Vol 30 (2) ◽

pp. 191-194 ◽

Cited By ~ 4

Author(s):

J D Johnston ◽

S Koneru ◽

T Kuwana ◽

S B Rosalki

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Bone Density ◽

Femoral Neck ◽

Serum Levels ◽

Bone Alkaline Phosphatase ◽

Serum Enzymes ◽

Tartrate Resistant Acid Phosphatase ◽

Vertebral Bone ◽

Caucasian Women

Serum levels of bone-origin alkaline phosphatase and of tartrate-resistant acid phosphatase were measured in Caucasian women aged 41–69 years who had volunteered for bone densitometry. Bone alkaline phosphatase and tartrate-resistant acid phosphatase were inversely correlated with vertebral bone density and with femoral neck bone density. Bone alkaline phosphatase and acid phosphatase were also significantly correlated, consistent with the concept of ‘coupling’ between osteoblast and osteoclast activity.

Download Full-text

Distributions of Succinic Dehydrogenase, Acid Phosphatase and Alkaline Phosphatase in the Kidneys of Rats Made Hypertensive by Partial Constriction of the Abdominal Aorta

Acta Pathologica Microbiologica Scandinavica ◽

10.1111/j.1699-0463.1957.tb01006.x ◽

2009 ◽

Vol 41 (2) ◽

pp. 119-121 ◽

Cited By ~ 2

Author(s):

Olavi Eränkö ◽

Mikko Niemi

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Abdominal Aorta ◽

Succinic Dehydrogenase

Download Full-text

Correlation of acid phosphatase but not alkaline phosphatase activity with myelination in the developing mouse brain

International Journal of Biochemistry ◽

10.1016/0020-711x(93)90013-5 ◽

1993 ◽

Vol 25 (2) ◽

pp. 247-251 ◽

Cited By ~ 2

Author(s):

Tzi Bun Ng ◽

W.Y. Chan ◽

Patrick P.L. Tam

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Phosphatase Activity ◽

Mouse Brain ◽

Alkaline Phosphatase Activity ◽

Developing Mouse Brain

Download Full-text

Phosphatase synthesis in Klebsiella (Aerobacter) aerogenes growing in continuous culture

Biochemical Journal ◽

10.1042/bj1270087 ◽

1972 ◽

Vol 127 (1) ◽

pp. 87-96 ◽

Cited By ~ 28

Author(s):

P. G. Bolton ◽

A. C. R. Dean

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Continuous Culture ◽

Activity Profile ◽

Glucose Effect ◽

Ph Values ◽

Nitrophenyl Phosphate ◽

Wide Range ◽

Rate Of Hydrolysis ◽

Hydrolysis Of

1. Phosphatase synthesis was studied in Klebsiella aerogenes grown in a wide range of continuous-culture systems. 2. Maximum acid phosphatase synthesis was associated with nutrient-limited, particularly carbohydrate-limited, growth at a relatively low rate, glucose-limited cells exhibiting the highest activity. Compared with glucose as the carbon-limiting growth material, other sugars not only altered the activity but also changed the pH–activity profile of the enzyme(s). 3. The affinity of the acid phosphatase in glucose-limited cells towards p-nitrophenyl phosphate (Km 0.25–0.43mm) was similar to that of staphylococcal acid phosphatase but was ten times greater than that of the Escherichia coli enzyme. 4. PO43−-limitation derepressed alkaline phosphatase synthesis but the amounts of activity were largely independent of the carbon source used for growth. 5. The enzymes were further differentiated by the effect of adding inhibitors (F−, PO43−) and sugars to the reaction mixture during the assays. In particular, it was shown that adding glucose, but not other sugars, stimulated the rate of hydrolysis of p-nitrophenyl phosphate by the acid phosphatase in carbohydrate-limited cells at low pH values (<4.6) but inhibited it at high pH values (>4.6). Alkaline phosphatase activity was unaffected. 6. The function of phosphatases in general is discussed and possible mechanisms for the glucose effect are outlined.

Download Full-text

SIGNIFICANCE OF TRANSAMINASE ACTIVITY OF SERUM

PEDIATRICS ◽

10.1542/peds.24.3.360 ◽

1959 ◽

Vol 24 (3) ◽

pp. 360-361

Author(s):

SAMUEL P. BESSMAN

Keyword(s):

Alkaline Phosphatase ◽

Enzyme Activity ◽

Acid Phosphatase ◽

Transaminase Activity ◽

Specific Approach ◽

Normal Tissues ◽

The Past ◽

Glycolytic Activity ◽

Enzyme Characteristic ◽

Phosphatase Alkaline

THE MEASUREMENT of enzyme activity of serum as an indicator of disease has a long history in medicine. In the past, it has been the aim of the designers of these methods to make them as specific as possible for assay of an enzyme characteristic of a particular system or group of similar organs. Examples of these venerable tests are those for amylase, acid phosphatase, alkaline phosphatase and choline esterase in the serum. Warburg made the first departure from this specificity by demonstrating that the activity of triosephosphate dehydrogenase in the serum of animals with cancer was much greater than that of controls. This test was partially specific, for as Warburg had earlier shown, the glycolytic activity of tumors is much greater than that of normal tissues. The non-specific approach became extreme with the introduction of the measurement of the glutamic-oxalacetic transaminase reaction in the diagnosis of acute coronary disease.

Download Full-text

Effects of prednisolone metasulfabenzoate on the induction of DNase II in comparison to alkaline phosphatase and acid phosphatase activities in cultures of hela S3 cells

Steroids ◽

10.1016/0039-128x(76)90137-9 ◽

1976 ◽

Vol 27 (6) ◽

pp. 773-783

Author(s):

Haim Bustan ◽

Hanoch Slor

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Dnase Ii ◽

Phosphatase Activities ◽

Hela S3 ◽

Hela S3 Cells

Download Full-text

Acid phosphatase, alkaline phosphatase, and nitrate reductase activity of selected ectomycorrhizal fungi

Canadian Journal of Botany ◽

10.1139/b89-101 ◽

1989 ◽

Vol 67 (3) ◽

pp. 750-753 ◽

Cited By ~ 19

Author(s):

Iwan Ho

Keyword(s):

Alkaline Phosphatase ◽

Enzyme Activity ◽

Acid Phosphatase ◽

Nitrate Reductase ◽

Phosphatase Activity ◽

Ectomycorrhizal Fungi ◽

Acid Phosphatase Activity ◽

Nitrate Reductase Activity ◽

Reductase Activity ◽

Phosphatase Alkaline

Seventeen isolates, encompassing five genera and eight species of ectomycorrhizal fungi, were compared for acid phosphatase, alkaline phosphatase, and nitrate reductase activity. Isolates within species differed in enzyme activity and isozyme patterns by host specificity and site (as exemplified by the genus Suillus). Host and site may have affected phosphatase enzyme activity. Generally, the Douglas-fir associates, which dominate in mesic sites, have higher acid phosphatase activity than pine associates, which mostly occupy xeric sites; however, pine associates from mesic sites also have higher acid phosphatase activity (e.g., S. tomentosus). In four isolates of Amanita muscaria, the effect of site was also apparent. Two of them, which have significantly higher acid phosphatase activity than the others, were isolated from mesic sites. The isozyme pattern of the genus Suillus appeared to be separated by host groups. Other isolates with only one species also differed more or less by host groups. They shared at least one band within host groups, except for the two isolates of Paxillus involutus from different hosts. The P. involutus S-403 isolated from an orchard showed much higher nitrate reductase activity than all other isolates. No apparent differences in nitrate reductase activity were found between the other isolates.

Download Full-text