Filamentous microfungi in raw flax and cotton for textile industry and their ciliostatic activity on tracheal organ cultures in vitro

1996 ◽  
Vol 134 (2) ◽  
pp. 91-96 ◽  
Author(s):  
E. Piecková ◽  
Z. Jesenská
Keyword(s):  
Textile Industry ◽  
Organ Cultures

The Culture in vitro of Urogenital Organs of Pleurodeles waltlii

Development ◽  
1962 ◽  
Vol 10 (4) ◽  
pp. 465-470
Author(s):  
Charles L. Foote ◽  
Florence M. Foote
Keyword(s):  
Organ Culture ◽  
Germ Cells ◽  
Culture Medium ◽  
Developmental Stages ◽  
Rana Catesbeiana ◽  
Sex Differentiation ◽  
Organ Cultures ◽  
Tissue And Organ Culture ◽  
Pleurodeles Waltlii

Earlier reports (Foote & Foote, 1958a, b, 1959) describe growth and maintenance in vitro of larval organs, particularly gonads, of Rana catesbeiana and Xenopus laevis. Immature germ cells of both testes and ovaries are well maintained in vitro, especially if the culture medium is supplemented with watersoluble sex-hormonal substances, although germ cells in process of maturation become necrotic. Recently some urogenital organs from the salamander, Pleurodeles waltlii, have been grown in vitro. Tissues and organs from this amphibian might prove to be more suitable for tissue and organ culture investigations than those of Anurans. Animals at three different ages were used in this study: recently hatched larvae, metamorphosing animals, and adults. To determine whether sex differentiation would occur in vitro, trunk portions of young larvae of Pleurodeles waltlii of developmental stages 37–38 (Gallien & Durocher, 1957) were placed in organ cultures.

In Vitro Analysis of Pulmonary Inflammation Using Rat Lung Organ Cultures

1992 ◽  
Vol 18 (1) ◽  
pp. 55-67 ◽  
Author(s):  
Jeffrey S. Warren ◽  
Peter A. Barton
Keyword(s):  
Pulmonary Inflammation ◽  
Rat Lung ◽  
Organ Cultures ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals The Effects of Taurine on Permethrininduced Cytogenetic and Oxidative Damage in Cultured Human Lymphocytes

2012 ◽  
Vol 63 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Hasan Turkez ◽  
Elanur Aydin
Keyword(s):  
Oxidative Damage ◽  
Textile Industry ◽  
Human Lymphocytes ◽  
Sister Chromatid Exchanges ◽  
Dependent Manner ◽  
Beneficial Effects ◽  
Oxidative Damages ◽  
Total Oxidative Stress ◽  
Oxidative Effects

The Effects of Taurine on Permethrininduced Cytogenetic and Oxidative Damage in Cultured Human LymphocytesPermethrin (PM) is a common pyrethroid pesticide used to control pests in agriculture, forestry, horticulture, health care, homes, and textile industry. It is confirmed as a strong mutagen in animals and humans. Taurine (TA) is an amino acid found in mammalian tissues that protects the cell against DNA damage. In this study, we investigated whether supplementation of human lymphocyte cultures with TA (in the concentrations of 25 μg mL-1, 50 μg mL-1and 100 μg mL-1) provided any protection against PM toxicity applied in the concentration of 200 μg mL-1. Genotoxicity was assessed using the micronucleus (MN) and sister chromatid exchanges (SCE) tests. In addition, we measured the total antioxidant capacity (TAC) and total oxidative stress (TOS) levels in the plasma to determine oxidative effects. PM increased SCE and MN levels and altered TAC and TOS levels. TA alone did not affect SCE and MN levels compared to controls, regardless of the concentration applied. In addition, it increased TAC levels without changing TOS levels. Moreover, it significantly buffered the negative cytogenetic and oxidative effects induced by PM in a clear dose-dependent manner. In conclusion, this study is the first to evidence the beneficial effects of TA against PM-induced DNA and oxidative damagesin vitro.

Differential expression of neurotrophin receptors during renal development

Development ◽  
1993 ◽  
Vol 119 (4) ◽  
pp. 977-989 ◽  
Author(s):  
M. Durbeej ◽  
S. Soderstrom ◽  
T. Ebendal ◽  
C. Birchmeier ◽  
P. Ekblom
Keyword(s):  
Growth Factors ◽  
Antisense Oligonucleotides ◽  
Kidney Development ◽  
Developmental Stages ◽  
Current Data ◽  
Organ Cultures ◽  
Experimental Conditions ◽  
High Affinity ◽  
Epithelial Sheets

Early kidney differentiation is driven by local cell-cell interactions. The metanephrogenic mesenchyme stimulates the epithelial ureter bud to grow and branch, whereas the ureter bud stimulates the mesenchyme to convert into a new epithelium. These interactions may be dependent on local growth factors and their receptors. We studied the expression of receptors for nerve growth factors during kidney development. Expression of the low- and high-affinity receptors was cell-type specific. The low-affinity NGF receptor was found in the uninduced mesenchyme at early developmental stages, but in the glomerular podocytes at later developmental stages. In contrast, the high-affinity trkB receptor was found in the cortical mesenchyme cells that will differentiate into stroma. The trkC receptor was found only weakly expressed and in a few parts of the collecting ducts. The role of these receptors and c-ros, a receptor-type kinase expressed on the tip of the ureter bud, was studied by modified antisense oligonucleotides. However, we found that both sense, antisense and nonsense phosphorothioate oligonucleotides inhibited mouse and rat embryonic kidney development in vitro. The oligonucleotides appeared to be toxic for rodent embryonic kidneys in the experimental conditions that we used. Moreover, oligonucleotides did not penetrate well into the epithelial sheets in the organ cultures. We conclude that studies with phosphorothioate antisense oligonucleotides in organ cultures of embryonic kidneys should be interpreted with caution. Our current data do not allow us to not assign a function for the low- or high-affinity NGF receptors or c-ros in kidney development.

scholarly journals Root Bacteria Recruited by Phragmites australis in Constructed Wetlands Have the Potential to Enhance Azo-Dye Phytodepuration

2019 ◽  
Vol 7 (10) ◽  
pp. 384 ◽  
Author(s):  
Valentina Riva ◽  
Francesca Mapelli ◽  
Evdokia Syranidou ◽  
Elena Crotti ◽  
Redouane Choukrallah ◽  
...  
Keyword(s):  
Plant Growth ◽  
Phragmites Australis ◽  
Textile Industry ◽  
Azo Dye ◽  
Growth Promotion ◽  
Wide Spectrum ◽  
Reactive Black 5 ◽  
Potential Use

The microbiome associated with plants used in phytodepuration systems can boost plant growth and services, especially in ecosystems dealing with recalcitrant compounds, hardly removed via traditional wastewater (WW) treatments, such as azo-dyes used in textile industry. In this context, we aimed to study the cultivable microbiome selected by Phragmites australis plants in a Constructed Wetland (CW) in Morocco, in order to obtain candidate inoculants for the phytodepuration of azo-dye contaminated WW. A collection of 152 rhizospheric and endophytic bacteria was established. The strains were phylogenetically identified and characterized for traits of interest in the phytodepuration context. All strains showed Plant Growth Promotion potential in vitro and 67% of them significantly improved the growth of a model plant in vivo compared to the non bacterized control plants. Moreover, most of the isolates were able to grow in presence of several model micropollutants typically found in WW, indicating their potential use in phytodepuration of a wide spectrum of effluents. The six most promising strains of the collection were tested in CW microcosms alone or as consortium: the consortium and two single inocula demonstrated to significantly increase the removal of the model azo-dye Reactive Black 5 compared to the non bacterized controls.

Synthesis of taurocholate by rat fetal liver in organ culture: effects of cortisol in vitro.

1979 ◽  
Vol 237 (2) ◽  
pp. E177 ◽  
Author(s):  
T O Graham ◽  
D H Van Thiel ◽  
J M Little ◽  
R Lester
Keyword(s):  
Organ Culture ◽  
Dose Response ◽  
Incubation Medium ◽  
Response Pattern ◽  
Fetal Liver ◽  
Dry Weight ◽  
Organ Cultures ◽  
In Vitro Incubation ◽  
Culture Effects

Taurocholate production by fetal hepatic organ cultures was measured by radioimmunoassay. Taurocholate production was maximal on day 1 of in vitro incubation, but was demonstrable in organ cultures maintained for periods up to 15 days. Explants obtained from fetuses of 18 gestational days of age produced only 82 pmol taurocholate per milligram dry weight of tissue during the first 24 h of incubation. Explants obtained from fetuses 21 gestational days of age produced 1,043 pmol taurocholate per milligram dry weight. The presence of cortisol (2.0 X 10(-6) M) in the incubation medium increased synthesis of taurocholate by rat fetal liver in which total taurocholate rose 50-fold above control after 120 h of incubation. In increasing concentrations from 2.0 X 10(-9) M to 2.0 X 10(-7) M, cortisol produced an incremental rise in taurocholate. However, additional increases in cortisol dose failed to provide further stimulation, and taurocholate production was inhibited by cortisol concentrations of 2.0 X 10(-5) M. The results provide further validation for the technique of fetal hepatic organ culture. They demonstrate that taurocholate synthesis is increasing rapidly during the final stages of gestation and show that cortisol augments taurocholate synthesis in a dose-response pattern.

Modification of Thai Silk Fibroin Scaffolds by Gelatin Conjugation for Tissue Engineering

2008 ◽  
Vol 55-57 ◽  
pp. 685-688 ◽  
Author(s):  
J. Chamchongkaset ◽  
Sorada Kanokpanont ◽  
David L. Kaplan ◽  
Siriporn Damrongsakkul
Keyword(s):  
Tissue Engineering ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Textile Industry ◽  
Three Dimensional ◽  
Biological Properties ◽  
Salt Leaching ◽  
Biological Compatibility ◽  
Salt Crystals

Silk has been used commercially as biomedical sutures for decades. Recently silk fibroin, especially from Bombyx mori silkworm, has been explored for many tissue engineering applications such as bone and cartilage due to its impressive biological compatibility and mechanical properties. In Thailand, Thai native silkworms have been long cultivated. Distinct characteristics of cocoon Thai silk are its yellow color and coarse filament. There is more sericin in Thai silk than in other Bombyx mori silks. These characteristics provide Thai silk a unique texture for textile industry. It is therefore the aim of this study to develop three-dimensional silk fibroin-based scaffolds from Thai yellow cocoon “Nangnoi-Srisaket” of Bombyx mori silkworms using salt-leaching method. To enhance the biological properties, type A gelatin, the denature form of collagen having good biocompactibility, was used to conjugate with silk fibroin scaffolds. The pore size of salt-leached silk fibroin scaffold structure represented the size of salt crystals used (600-710µm). After gelatin conjugation, gelatin was partly formed fibers inside the pores of silk fibroin scaffolds resulting in fiber-like structure with highly interconnection. Gelatin conjugation enhanced the compressive modulus of silk fibroin scaffolds by 93%. The results on in vitro culture using mouse osteoblast-like cells (MC3T3-E1) showed that gelatin conjugation could promote the cell proliferation in silk fibroin scaffolds. Moreover, the observed morphology of cells proliferated inside the scaffold after 14 days of culture showed the larger spreading area of cells on conjugated gelatin/silk fibroin scaffolds, compared to round-shaped cells on silk fibroin scaffolds. The results implied that Thai silk fibroin looked promising to be applied in tissue engineering and gelatin conjugation on Thai silk fibroin scaffolds could enhance the biological properties of scaffolds.

scholarly journals Organ cultures of embryonic rat tongue support tongue and gustatory papilla morphogenesis in vitro without intact sensory ganglia

1997 ◽  
Vol 377 (3) ◽  
pp. 324-340 ◽  
Author(s):  
Joseph-Pascal Mbiene ◽  
Donald K. Maccallum ◽  
Charlotte M. Mistretta
Keyword(s):  
Sensory Ganglia ◽  
Organ Cultures ◽  
Morphogenesis In Vitro ◽  
Rat Tongue

Tissue Culture of Human Adult Adenoids and of Middle Ear Mucosa

1976 ◽  
Vol 85 (3) ◽  
pp. 327-333 ◽  
Author(s):  
Ilana Drucker ◽  
Ziva Weisman ◽  
Jacob Sadé
Keyword(s):  
Middle Ear ◽  
Ciliary Activity ◽  
Organ Cultures ◽  
Middle Ear Mucosa ◽  
Monolayer Growth ◽  
Stratified Squamous Epithelium ◽  
Inducing Factors ◽  
Developmental Characteristics ◽  
Mucociliary Epithelia

The increased number of mucus producing cells as well as the presence of stratified squamous epithelium in pathological and experimental middle ear conditions, point towards the possibility of metaplastic changes of the middle ear mucosa, similar to the metaplastic capabilities of respiratory mucosae in general, as observed clinically or provoked experimentally. The purpose of this study was to develop a model of postembryonic human respiratory mucosae, in vitro, for the study of triggering or inducing factors involved in its normal and metaplastic differentiation. Explants from adenoids and middle ear mucosa were cultured, both as organ cultures and monolayers, for periods of up to two weeks, and their developmental characteristics were studied and described. Over 50% of the explants showed mitosis, epithelial and monolayer growth, ciliary activity and differentiation into ciliated and into mucus-producing cells. Adenoid explants were grown in air without and with added 5% CO2. Under the latter conditions, the proportion of explants and monolayers showing ciliary activity was 50% greater. It is concluded that this model might be suitable for further studies of the factors which control cyto-differentiation in mucociliary epithelia. Maintaining its growth for a longer period would, however, be desirable.

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