Influence of the conditions of trypsin immobilization onto Spherosil on coupling efficiency

1978 ◽  
Vol 5 (1) ◽  
pp. 1-11 ◽  
Author(s):  
P. Monsan
Keyword(s):  
Coupling Efficiency

Coupling efficiency and sensitivity of an LED to a single-mode fibre

1986 ◽  
Vol 22 (21) ◽  
pp. 1110 ◽  
Author(s):  
D.N. Christodoulides ◽  
L.A. Reith ◽  
M.A. Saifi
Keyword(s):  
Coupling Efficiency ◽  
Single Mode ◽  
Single Mode Fibre ◽  
Mode Fibre

scholarly journals Bioenergetic and Proteomic Profiling of Immune Cells in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Patients: An Exploratory Study

Biomolecules ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 961
Author(s):  
Paula Fernandez-Guerra ◽  
Ana C. Gonzalez-Ebsen ◽  
Susanne E. Boonen ◽  
Julie Courraud ◽  
Niels Gregersen ◽  
...  
Keyword(s):  
Chronic Fatigue Syndrome ◽  
Complex Disease ◽  
Mononuclear Cells ◽  
Coupling Efficiency ◽  
Well Being ◽  
Chronic Fatigue ◽  
Myalgic Encephalomyelitis ◽  
Flux Analysis ◽  
Proteomic Profiling ◽  
Fatigue Syndrome

Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a heterogeneous, debilitating, and complex disease. Along with disabling fatigue, ME/CFS presents an array of other core symptoms, including autonomic nervous system (ANS) dysfunction, sustained inflammation, altered energy metabolism, and mitochondrial dysfunction. Here, we evaluated patients' symptomatology and the mitochondrial metabolic parameters in peripheral blood mononuclear cells (PBMCs) and plasma from a clinically well-characterised cohort of six ME/CFS patients compared to age- and gender-matched controls. We performed a comprehensive cellular assessment using bioenergetics (extracellular flux analysis) and protein profiles (quantitative mass spectrometry-based proteomics) together with self-reported symptom measures of fatigue, ANS dysfunction, and overall physical and mental well-being. This ME/CFS cohort presented with severe fatigue, which correlated with the severity of ANS dysfunction and overall physical well-being. PBMCs from ME/CFS patients showed significantly lower mitochondrial coupling efficiency. They exhibited proteome alterations, including altered mitochondrial metabolism, centred on pyruvate dehydrogenase and coenzyme A metabolism, leading to a decreased capacity to provide adequate intracellular ATP levels. Overall, these results indicate that PBMCs from ME/CFS patients have a decreased ability to fulfill their cellular energy demands.

scholarly journals Cell-Permeable Succinate Rescues Mitochondrial Respiration in Cellular Models of Statin Toxicity

2021 ◽  
Vol 22 (1) ◽  
pp. 424
Author(s):  
Vlad F. Avram ◽  
Imen Chamkha ◽  
Eleonor Åsander-Frostner ◽  
Johannes K. Ehinger ◽  
Romulus Z. Timar ◽  
...  
Keyword(s):  
Oxygen Consumption ◽  
Mitochondrial Dysfunction ◽  
Mitochondrial Respiration ◽  
Complex I ◽  
Ex Vivo ◽  
Coupling Efficiency ◽  
Human Platelets ◽  
Lipid Lowering ◽  
Lipid Lowering Therapy ◽  
Cellular Models

Statins are the cornerstone of lipid-lowering therapy. Although generally well tolerated, statin-associated muscle symptoms (SAMS) represent the main reason for treatment discontinuation. Mitochondrial dysfunction of complex I has been implicated in the pathophysiology of SAMS. The present study proposed to assess the concentration-dependent ex vivo effects of three statins on mitochondrial respiration in viable human platelets and to investigate whether a cell-permeable prodrug of succinate (complex II substrate) can compensate for statin-induced mitochondrial dysfunction. Mitochondrial respiration was assessed by high-resolution respirometry in human platelets, acutely exposed to statins in the presence/absence of the prodrug NV118. Statins concentration-dependently inhibited mitochondrial respiration in both intact and permeabilized cells. Further, statins caused an increase in non-ATP generating oxygen consumption (uncoupling), severely limiting the OXPHOS coupling efficiency, a measure of the ATP generating capacity. Cerivastatin (commercially withdrawn due to muscle toxicity) displayed a similar inhibitory capacity compared with the widely prescribed and tolerable atorvastatin, but did not elicit direct complex I inhibition. NV118 increased succinate-supported mitochondrial oxygen consumption in atorvastatin/cerivastatin-exposed platelets leading to normalization of coupled (ATP generating) respiration. The results acquired in isolated human platelets were validated in a limited set of experiments using atorvastatin in HepG2 cells, reinforcing the generalizability of the findings.

High Power and Stable High Coupling Efficiency (66%) Superluminescent Light Emitting Diodes by Using Active Multi-Mode Interferometer

2011 ◽  
Vol E94-C (5) ◽  
pp. 862-864 ◽  
Author(s):  
Zhigang ZANG ◽  
Keisuke MUKAI ◽  
Paolo NAVARETTI ◽  
Marcus DUELK ◽  
Christian VELEZ ◽  
...  
Keyword(s):  
High Power ◽  
Light Emitting Diodes ◽  
Coupling Efficiency ◽  
Light Emitting ◽  
Multi Mode

scholarly journals Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 638
Author(s):  
Monika Mazur ◽  
Daria Wojciechowska ◽  
Ewa Sitkiewicz ◽  
Agata Malinowska ◽  
Bianka Świderska ◽  
...  
Keyword(s):  
Life Cycle ◽  
Developmental Stages ◽  
Coupling Efficiency ◽  
Slime Mold ◽  
Intact Cells ◽  
Life Cycle Stages ◽  
Early Developmental Stages ◽  
Proteomic Study ◽  
And Function ◽  
Early Developmental

The slime mold Dictyostelium discoideum’s life cycle includes different unicellular and multicellular stages that provide a convenient model for research concerning intracellular and intercellular mechanisms influencing mitochondria’s structure and function. We aim to determine the differences between the mitochondria isolated from the slime mold regarding its early developmental stages induced by starvation, namely the unicellular (U), aggregation (A) and streams (S) stages, at the bioenergetic and proteome levels. We measured the oxygen consumption of intact cells using the Clarke electrode and observed a distinct decrease in mitochondrial coupling capacity for stage S cells and a decrease in mitochondrial coupling efficiency for stage A and S cells. We also found changes in spare respiratory capacity. We performed a wide comparative proteomic study. During the transition from the unicellular stage to the multicellular stage, important proteomic differences occurred in stages A and S relating to the proteins of the main mitochondrial functional groups, showing characteristic tendencies that could be associated with their ongoing adaptation to starvation following cell reprogramming during the switch to gluconeogenesis. We suggest that the main mitochondrial processes are downregulated during the early developmental stages, although this needs to be verified by extending analogous studies to the next slime mold life cycle stages.

scholarly journals Coherent characterisation of a single molecule in a photonic black box

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Sebastien Boissier ◽  
Ross C. Schofield ◽  
Lin Jin ◽  
Anna Ovvyan ◽  
Salahuddin Nur ◽  
...  
Keyword(s):  
Single Molecule ◽  
Coupling Efficiency ◽  
Black Box ◽  
Measured Spectrum ◽  
Photonic Structure ◽  
Quantum Emitter ◽  
Precise Knowledge ◽  
Transmission And Reflection Spectra ◽  
Waveguide Coupling ◽  
Transmission And Reflection

AbstractExtinction spectroscopy is a powerful tool for demonstrating the coupling of a single quantum emitter to a photonic structure. However, it can be challenging in all but the simplest of geometries to deduce an accurate value of the coupling efficiency from the measured spectrum. Here we develop a theoretical framework to deduce the coupling efficiency from the measured transmission and reflection spectra without precise knowledge of the photonic environment. We then consider the case of a waveguide interrupted by a transverse cut in which an emitter is placed. We apply that theory to a silicon nitride waveguide interrupted by a gap filled with anthracene that is doped with dibenzoterrylene molecules. We describe the fabrication of these devices, and experimentally characterise the waveguide coupling of a single molecule in the gap.

scholarly journals Fabrication of Graphene Nanomesh FET Terahertz Detector

Micromachines ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 641
Author(s):  
Yuan Zhai ◽  
Yi Xiang ◽  
Weiqing Yuan ◽  
Gang Chen ◽  
Jinliang Shi ◽  
...  
Keyword(s):  
Room Temperature ◽  
Energy Gap ◽  
Coupling Efficiency ◽  
High Sensitivity ◽  
Fabrication Process ◽  
Monolayer Graphene ◽  
Terahertz Waves ◽  
Wet Process ◽  
Graphene Nanomesh ◽  
Thz Detector

High sensitivity detection of terahertz waves can be achieved with a graphene nanomesh as grating to improve the coupling efficiency of the incident terahertz waves and using a graphene nanostructure energy gap to enhance the excitation of plasmon. Herein, the fabrication process of the FET THz detector based on the rectangular GNM (r-GNM) is designed, and the THz detector is developed, including the CVD growth and the wet-process transfer of high quality monolayer graphene films, preparation of r-GNM by electron-beam lithography and oxygen plasma etching, and the fabrication of the gate electrodes on the Si3N4 dielectric layer. The problem that the conductive metal is easy to peel off during the fabrication process of the GNM THz device is mainly discussed. The photoelectric performance of the detector was tested at room temperature. The experimental results show that the sensitivity of the detector is 2.5 A/W (@ 3 THz) at room temperature.

scholarly journals Investigation of serial coherent laser beam combination based on Brillouin amplification

2007 ◽  
Vol 25 (1) ◽  
pp. 79-83 ◽  
Author(s):  
SHUANGYI WANG ◽  
ZHIWEI LÜ ◽  
DIANYANG LIN ◽  
LEI DING ◽  
DONGBIN JIANG
Keyword(s):  
Laser Beam ◽  
High Power ◽  
Simple Structure ◽  
Low Cost ◽  
Coupling Efficiency ◽  
Laser Beams ◽  
Beam Combination ◽  
Multiple Beams ◽  
Combination Scheme ◽  
Stokes Beam

Based on transferring energy from multiple pump beams into one Stokes beam using Brillouin amplification, a serial coherent laser beam combination scheme is presented, which has many advantages, such as, simple structure, low cost, ease of adjustment, higher load capability, scalable easily, etc. Furthermore, it has been demonstrated that the combination of several beams using this method is theoretically possible. But in practice, the amplification of high power Stokes beam is a key problem to solve. In this paper, the amplification of Stokes beam whose power is higher than the pump beam is first studied and proved experimentally. Coupling the two laser beams by this method is proved experimentally, and the coupling efficiency reaches more than 80%. Then the feasibility of multiple beams combination based on Brillouin amplification is analyzed and tested theoretically.

High optical coupling efficiency quasi-vertical taper for polymer waveguide devices

2015 ◽  
Author(s):  
Zeyu Pan ◽  
Harish Subbaraman ◽  
Yi Zou ◽  
Xingyu Zhang ◽  
Cheng Zhang ◽  
...  
Keyword(s):  
Coupling Efficiency ◽  
Optical Coupling ◽  
Polymer Waveguide ◽  
Waveguide Devices ◽  
Optical Coupling Efficiency
Sign in / Sign up

Export Citation Format

Share Document