Electron-autoradiographic study of RNA synthesis in epithelial cells of the renal tubules of albino rats poisoned with mercuric chloride

This thesis studied the cytotoxicity of mercuric chloride on human epihelial cells. The three detection techniques were developed to monitor the cytotoxicity of soluble mercuric chloride to human health. Both increased concentration and exposure time resulted in increased DNA damage and cell death. At lower levels death occurred by a mixture of apoptosis and necrosis, while at higher levels cell death occurred primarily by necrosis. This is the first study to demonstrate a deleterious effect of soluble mercuric chloride on human epithelial cells, although mercury has long been known as nephrotoxic and neurotoxic.

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Nephrotoxicological Effect of Water Soluble Fraction of Bonny Light Crude Oil in Wistar Albino Rats

International Journal of Biochemistry Research & Review ◽

10.9734/ijbcrr/2019/v28i430151 ◽

2019 ◽

pp. 1-9

Author(s):

Abraham, Chiedozie Nicholas ◽

J. Udom, Godwin ◽

C. Patrick-Iwuanyanwu, Kingsley

Keyword(s):

Crude Oil ◽

Soluble Fraction ◽

Water Soluble ◽

Albino Rats ◽

Renal Tubules ◽

Water Soluble Fraction ◽

Light Crude Oil ◽

Range Finding ◽

Effect Of Water ◽

Wistar Albino Rats

This study evaluated the Nephrotoxic effect of water soluble fraction (WSF) of Bonny Light Crude Oil (BLCO). After preparation of the WSF and a range finding test, the Wistar albino rats were administered three concentrations (25%, 50% and 100%) of WSF of BLCO for 30 and 60days. Data from the study showed that Urea concentration increased significantly (p≤0.05) with increasing dose of BLCO ranging from 14.71 mg/dl in the control to 35.28 mg/dl in the 100% group after 30days and 14.28 mg/dl in the control to 41.08mg/dl in the 100% group after 60days, Creatinine concentration increased significantly (p≤0.05) from 0.22 mg/dl in the control to 0.82mg/dl in the 100% group after 60 days administration while electrolyte (Na, K, Cl) concentration increased significantly (p≤0.05) with increasing dose of BLCO after 60days administration. Histopathological examination of the kidney was characterized by partial partitioning of the glomerular tufts, obliteration of the Bowman’s capsule and distortion of the renal tubules. The findings in this research suggest that WSF of BLCO induced nephrotoxicity.

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HISTOLOGICAL STUDY OF THE POSSIBLE PROTECTIVE EFFECT OF GARLIC OIL ON SULFASALAZINE INDUCED INJURY OF RENAL TUBULES IN ADULT MALE ALBINO RATS.

Egyptian Journal of Histology ◽

10.21608/ejh.2021.98665.1572 ◽

2021 ◽

Vol 0 (0) ◽

pp. 0-0

Author(s):

fouad heikal ◽

wafaa Abdel rahman ◽

hoda khalifa ◽

Rasha Eldokmak ◽

Elsayed Metwally

Keyword(s):

Protective Effect ◽

Adult Male ◽

Histological Study ◽

Albino Rats ◽

Renal Tubules ◽

Garlic Oil

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The Antioxidant Efficacy of Wheatgrass (Triticum Aestivum) on Mercuric Chloride (HgCl2) - Induced Oxidative Stress in Rat Model

Current Research in Nutrition and Food Science Journal ◽

10.12944/crnfsj.9.2.09 ◽

2021 ◽

Vol 9 (2) ◽

pp. 450-464

Author(s):

Renu Tripathi ◽

Swati Agarwal ◽

Syed Ibrahim Rizvi ◽

Neetu * Mishra

Keyword(s):

Oxidative Stress ◽

Mercuric Chloride ◽

Rat Model ◽

Antioxidant Status ◽

Protective Efficacy ◽

Density Lipoprotein ◽

Control Group ◽

Albino Rats ◽

Antioxidant Power ◽

Group I

Mercury is a harmful toxic pollutant, which has hepato-nephrotoxic, hematotoxic, genotoxic and neurotoxic, effects. The aim of the study was to evaluate the protective efficacy of wheatgrass on mercuric chloride (HgCl2) induced oxidative stress and associated complications in rat model. Albino rats were divided into four groups (three rats per group). Group I normal control group. Group II oxidative stressed group received mercuric chloride (0.5 mg/kg/day). Group III only received wheatgrass extract (100 mg/kg/day), whereas Group IV received wheatgrass (100 mg/kg/day) after one hour, followed by mercuric chloride (0.5 mg/kg/day) for 30 days. The results of the study showed that wheatgrass supplementation significantly decreased the HgCl2 induced elevated oxidative stress parameters Plasma Malondialdehyde (MDA) content, Plasma membrane redox system (PMRS), Advanced oxidation protein products (AOPP), simultaneously elevated lipid profile (Total Cholesterol, Triglycerides, Low-density lipoprotein (LDL), liver enzymes as, Plasma Alkaline phosphatase (ALP), Aspartate aminotransferase (AST), and Alanine aminotransferase (ALT), Serum Urea, and Creatinine levels in rats. In addition, wheatgrass treatment improved the antioxidant status in terms of intracellular Reduced Glutathione (GSH), Ferric reducing antioxidant power (FRAP) and 2, 2- diphenyl -1- picrylhydrazyl (DPPH). Therefore it can be concluded that wheatgrass has great potential to diminish the stress-mediated complications and improve the antioxidant status.

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Assessment of the Protective Effect of Lepidium sativum against Aluminum-Induced Liver and Kidney Effects in Albino Rat

BioMed Research International ◽

10.1155/2019/4516730 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Maha Jameal Balgoon

Keyword(s):

Structural Changes ◽

Lepidium Sativum ◽

Albino Rats ◽

Renal Tubules ◽

Albino Rat ◽

Antioxidant Power ◽

Superior Effect ◽

Ferric Reducing Antioxidant Power ◽

Liver And Kidney ◽

Induced Changes

Background and Objectives. Environmental pollution with the different Aluminum (Al) containing compounds has been increased. Liver and kidney are two vital organs targeted by Al accumulation. The aim of this study was to assess the possible protective and curative effects of Lepidium sativum Linn (LS) against Al-induced impairment of liver and kidney in albino rat and to explore the mechanism behind this effect. Materials and Methods. This experimental animal-based study included fifty albino rats divided into five groups, the control, LS-treated (20 mg/kg), AlCl3-treated (10 mg/kg), AlCl3 then LS, and AlCl3 plus LS-treated, simultaneously for 8 weeks. At the end of the experiment, hepatic and renal functions as well as the biomarkers of antioxidants activities were assessed in the serum. Both liver and kidney were dissected out and histopathologically examined. Results. This study showed that administration of AlCl3 caused a significant (p<0.05) reduction in rats body weight. It significantly increased serum AST, ALT, ALP, bilirubin, urea, and creatinine levels and decreased total protein and albumin. AlCl3 significantly reduced enzymatic (catalase), nonenzymatic (reduced glutathione), and ferric reducing antioxidant power (FRAP) in the serum. Histopathologically, it induced necrosis and degeneration of hepatocytes, glomeruli, and renal tubules. Administration of LS after or along with AlCl3 significantly restored the serum biomarkers of liver and kidney functions to their near-normal levels and had the ability to overcome Al-induced oxidative stress and preserved, to some extent, the normal hepatic and renal structure. The coadministration of LS had a superior effect in alleviating Al-induced changes. Conclusion. Exposure to AlCl3 induced a set of functional and structural changes in the liver and kidney of rats evident through both biochemical and histopathological assessment. The antioxidant activity of LS seeds mediated a protective and curative effect of LS against such changes. Further study through a rigorous clinical trial to prove LS activity on human is recommended.

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