Previous work in our laboratory and also reported in this meeting has indicated that insecticidal controlled atmospheres at high temperatures (0.5% O2 + 50% CO2 at 44-55°C and 50% RH) are very effective in causing in vitro mortality of eggs and third instar larvas of Anastrepaha ludens and A. obliqua. This work is a follow up that evaluated the effect of such atmospheres on the in vivo mortality of third instar larvas artificially infested in mango. Atmospheres evaluated included 0% O2+ 50% CO2 at 35, 37, 39, 40, 42, 43, 44, 45, 46, 47, 48, and 49°C for 160 min. Treatments at 35-40°C caused 100% mortality of larvas of A. obliqua, but not of A. ludens. Temperatures of 42 to 49°C caused 100% mortality of larvas of both species. Statistical analysis to calculate the probit 9 will be discussed.