The effect of liposomal cefoperazone againstPseudomonas aeruginosa in a granulocytopenic mouse model of acute lung infection

Infection ◽  
1992 ◽  
Vol 20 (6) ◽  
pp. 360-364 ◽  
Author(s):  
P. H. Di Rocco ◽  
M. C. Nacucchio ◽  
F. Mancuso ◽  
D. O. Sordelli ◽  
Anne Morris Hooke
Keyword(s):  
Mouse Model ◽  
Lung Infection

scholarly journals Imaging of bioluminescent Klebsiella pneumoniae induced pulmonary infection in an immunosuppressed mouse model

2020 ◽  
Vol 48 (10) ◽  
pp. 030006052095647
Author(s):  
Xing Hu ◽  
Yun Cai ◽  
Yuhang Wang ◽  
Rui Wang ◽  
Jin Wang ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Mouse Model ◽  
Real Time ◽  
Imaging System ◽  
Lung Infection ◽  
Infection Model ◽  
Immunodeficient Mice ◽  
Infection Models ◽  
Infection Disease ◽  
Immunocompetent Mice

Objective To establish a mouse model of bioluminescent Klebsiella pneumoniae-induced lung infection, under different infection states after pretreatment with various dosages of cyclophosphamide (CTX). Methods A K. pneumoniae strain carrying the luxCDABE operon was used to infect immunocompetent mice (intraperitoneal injection of saline at 4 days and 1 day prior to experimental lung infection) and immunodeficient mice (50 mg/kg CTX at 4 days and 50 mg/kg CTX at 1 day prior to lung infection; or 150 mg/kg CTX at 4 days and 100 mg/kg CTX at 1 day prior to lung infection). Disease progression was monitored in living mice using a bioluminescence imaging system. The bioluminescent images, bacterial loads in lungs, blood cytological changes and histopathology of lungs were analysed. Results K. pneumoniae-induced lung infection models were established in mice pretreated with CTX. Different doses of CTX led to different severities of lung infection. Mice pretreated with 150/100 mg/kg CTX were more suitable for real-time monitoring as they had more typical bioluminescent images of lung infection, more obvious changes in the bioluminescent intensity values, more bacterial colonies in the lungs and more distinct pulmonary pathological changes. Conclusions A stable bioluminescent K. pneumonia-induced lung infection model was successfully established in mice pretreated with CTX, which can be semi-quantitatively monitored in real-time.

scholarly journals Curcumin alone and in combination with augmentin protects against pulmonaryinflammation and acute lung injury generated during Klebsiella pneumoniae B5055-induced lung infection in BALB/c mice

2010 ◽  
Vol 59 (4) ◽  
pp. 429-437 ◽  
Author(s):  
Shruti Bansal ◽  
Sanjay Chhibber
Keyword(s):  
Klebsiella Pneumoniae ◽  
Mouse Model ◽  
Lung Tissue ◽  
Inflammatory Mediators ◽  
Bacterial Load ◽  
Neutrophil Infiltration ◽  
Lung Infection ◽  
Control Group ◽  
Intranasal Route ◽  
Anti Inflammatory

Acute lung injuries due to acute lung infections remain a major cause ofmortality. Thus a combination of an antibiotic and a compound with immunomodulatoryand anti-inflammatory activities can help to overcome acute lung infection-inducedinjuries. Curcumin derived from the rhizome of turmeric has been used fordecades and it exhibits anti-inflammatory, anti-carcinogenic, immunomodulatoryproperties by downregulation of various inflammatory mediators. Keeping theseproperties in mind, we investigated the anti-inflammatory properties of curcuminin a mouse model of acute inflammation by introducing Klebsiella pneumoniae B5055 into BALB/c mice via the intranasal route. Intranasal instillationof bacteria in this mouse model of acute pneumonia-induced inflammation resultedin a significant increase in neutrophil infiltration in the lungs along withincreased production of various inflammatory mediators [i.e. malondialdehyde (MDA),myeloperoxidase (MPO), nitric oxide (NO), tumour necrosisfactor (TNF)-α] in the lung tissue. The animalsthat received curcumin alone orally or in combination with augmentin, 15 daysprior to bacterial instillation into the lungs via the intranasal route, showeda significant (P <0.05) decrease in neutrophil influxinto the lungs and a significant (P <0.05) decreasein the production of MDA, NO, MPO activity and TNF-α levels.Augmentin treatment alone did not decrease the MDA, MPO, NO and TNF-α levels significantly (P >0.05) as compared tothe control group. We therefore conclude that curcumin ameliorates lung inflammationinduced by K. pneumoniae B5055 without significantly (P <0.05) decreasing the bacterial load in the lung tissue whereasaugmentin takes care of bacterial proliferation. Hence, curcumin can be usedas an adjunct therapy along with antibiotics as an anti-inflammatory or animmunomodulatory agent in the case of acute lung infection.

scholarly journals Multitracer Stable Isotope Quantification of Arginase and Nitric Oxide Synthase Activity in a Mouse Model of Pseudomonas Lung Infection

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Hartmut Grasemann ◽  
Thomas Jaecklin ◽  
Anne Mehl ◽  
Hailu Huang ◽  
Mahroukh Rafii ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Stable Isotope ◽  
Mouse Model ◽  
Lung Infection ◽  
Lung Perfusion ◽  
Arginase Activity ◽  
Mouse Lung ◽  
Perfusion Model ◽  
Isolated Lung Perfusion ◽  
Isolated Lung

Cystic fibrosis airways are deficient for L-arginine, a substrate for nitric oxide synthases (NOSs) and arginases. The rationale for this study was to quantify NOS and arginase activity in the mouse lung. Anesthetized unventilated mice received a primed constant stable isotope intravenous infusion containing labeled L-arginine, ornithine, and citrulline. The isotopic enrichment of each of the infused isotopomers and its product amino acids were measured in plasma and organ homogenates using liquid chromatography-tandem mass spectrometry. The effect of infection was studied three days after direct tracheal instillation of Pseudomonas-coated agar beads. In the infusion model, lung infection resulted in a significant (28-fold) increase in NOS activity in lung but not in trachea, kidney, liver, or plasma. Absolute rates of arginase activity in solid tissues could not be calculated in this model. In an isolated lung perfusion model used for comparison increased NOS activity in infected lungs was confirmed (28.5-fold) and lung arginase activity was increased 9.7-fold. The activity of L-arginine metabolizing enzymes can be measured using stable isotope conversion in the mouse. Accumulation of L-ornithine in the whole mouse model hindered the exact quantification of arginase activity in the lung, a problem that was overcome utilizing an isolated lung perfusion model.

scholarly journals Efficacy of Benzoxazinorifamycins in a Mouse Model of Chlamydia pneumoniae Lung Infection

2008 ◽  
Vol 52 (5) ◽  
pp. 1855-1858 ◽  
Author(s):  
Lee Ann Campbell ◽  
Cho-Chou Kuo ◽  
Robert J. Suchland ◽  
David M. Rothstein
Keyword(s):  
Body Weight ◽  
Mouse Model ◽  
Chlamydia Pneumoniae ◽  
Lung Infection ◽  
New Chemical Entities

ABSTRACT The efficacy of rifalazil and other benzoxazinorifamycins was tested in a mouse model of lung infection against Chlamydia pneumoniae. Rifalazil and six related new chemical entities all showed efficacy after one dose per day for 3 days at either 3 or 1 mg/kg of body weight.

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