An LCEC method for the analysis of synthetic amino acids in fruit juices

1990 ◽  
Vol 29 (1-2) ◽  
pp. 51-53 ◽  
Author(s):  
A. Dyremark ◽  
M. Ericsson

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2756-2767
Author(s):  
Vijaya Vemani ◽  
Mounika P ◽  
Poulami Das ◽  
Anand Kumar Tengli

In the preservation of normal physiological functions, the building blocks of the body called amino acids play a crucial role. A number of valuable and nutritional phytoconstituents are contained in fruit juices, such as vitamins, minerals, microelements, organic acids, antioxidants, flavonoids, amino acids and other components. Due to the growing population and demand, the quality of fruit juices is decreasing. One of the unethical and harmful practices called adulteration or food fraudulence has been adopted by most food and beverage industries. The amino acids which is one of the most important phytochemicals of fruit and fruit juices which affects the organoleptic properties like color, odor, and taste of juices and also helps in authenticity process from governing bodies by providing total amino acid content. Consequently, the main aim of the present review work is to provide information regarding the importance of amino acids, how they are adulterated, the potential analytical approach to detected amino acids and which methods are generally accepted method by the food industries. According to the literature review, we presume that reverse phased high-performance liquid chromatography with pre-column derivatization was the most adopted method for quality checking due to its advantages over other old and recent analytical approaches like simple, rapid, cost-effective nature, less / no sample matrix effect with high sensitivity, accuracy, and precision.



2003 ◽  
Vol 77 (1) ◽  
pp. 3-10 ◽  
Author(s):  
L. A. Rubiot

AbstractThree experiments were carried out to determine plasma amino acids concentrations in circulating, portal and hepatic blood of growing male Wistar rats given diets containing lactalbumin, faba beans or chickpeas as the only protein source. Diets contained the same amount of digestible energy (15·5 kJ/g) and protein (lactalbumin in controls or legume proteins in the experimental diets; 100 g/kg). Appropriate amounts of essential synthetic amino acids were also added to legume-based diets taking into account their amino acid composition to equalize them to control (lactalbumin) diets. Portal blood flow (8·7±0·3 ml/min) was measured by using a transit-time ultrasound flow probe. Higher (P < 001) plasma concentrations of methionine than of controls were determined in hepatic veins of legume-fed rats. In contrast, lower (P < 001) concentrations of threonine, proline, valine, leucine, phenylalanine and lysine than those of controls were found in faba bean- and chickpea-fed rats. The same result as hepatic was obtained for portal and circulating plasma samples except that alanine and histidine values of legume-fed rats were also lower (P < 001) than controls. Calculated net afferent appearance rates of amino acids to the liver were lower (P < 001) than controls in rats given faba bean and chickpea diets for threonine, alanine, proline, valine, leucine, phenylalanine and lysine. This lower contribution of amino acids to the liver mainly via the portal vein in faba bean or chickpea-fed rats might explain previously reported differences in protein utilization and growth in comparison with animals given other protein sources (lactalbumin).



Author(s):  
Nurit Haspel ◽  
Jie Zheng ◽  
Carlos Aleman ◽  
David Zanuy ◽  
Ruth Nussinov


1990 ◽  
Vol 38 (8) ◽  
pp. 1648-1651 ◽  
Author(s):  
Ibolya Molnar-Perl ◽  
Mendel Friedman


2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Kirsten K. Ahring ◽  
Allan M. Lund ◽  
Erik Jensen ◽  
Thomas G. Jensen ◽  
Karen Brøndum-Nielsen ◽  
...  

Introduction. Management of phenylketonuria (PKU) is achieved through low-phenylalanine (Phe) diet, supplemented with low-protein food and mixture of free-synthetic (FS) amino acid (AA). Casein glycomacropeptide (CGMP) is a natural peptide released in whey during cheese-making and does not contain Phe. Lacprodan® CGMP-20 used in this study contained a small amount of Phe due to minor presence of other proteins/peptides.Objective. The purpose of this study was to compare absorption of CGMP-20 to FSAA with the aim of evaluating short-term effects on plasma AAs as well as biomarkers related to food intake.Methods. This study included 8 patients, who had four visits and tested four drink mixtures (DM1–4), consisting of CGMP, FSAA, or a combination. Plasma blood samples were collected at baseline, 15, 30, 60, 120, and 240 minutes (min) after the meal. AA profiles and ghrelin were determined 6 times, while surrogate biomarkers were determined at baseline and 240 min. A visual analogue scale (VAS) was used for evaluation of taste and satiety.Results. The surrogate biomarker concentrations and VAS scores for satiety and taste were nonsignificant between the four DMs, and there were only few significant results for AA profiles (not Phe).Conclusion. CGMP and FSAA had the overall same nonsignificant short-term effect on biomarkers, including Phe. This combination of FSAA and CGMP is a suitable supplement for PKU patients.



1985 ◽  
Vol 65 (1) ◽  
pp. 205-210 ◽  
Author(s):  
S. LEESON ◽  
J. D. SUMMERS

Leghorn pullets were reared on one of four dietary programs formulated to (1) conventional least-cost, (2) least-cost to amino acid requirements with no specification for crude protein, (3) as (2) with cost of protein feedstuffs increased 40% and (4) corn-soybean meal. All diets contained 18% and 15% CP, or equivalents, for 0–6 and 6–18 wk, respectively. Diets were offered ad-libitum to 14 replicate groups of 10 caged pullets. Pullets fed the conventional least-cost and corn-soybean diets were heavier (P < 0.05) at 6 wk of age than pullets reared on either of the two diets formulated to amino acid specifications. At 12 and 18 wk of age, conventionally fed birds were heavier than pullets fed amino-acid-based diets, while all groups were heavier than birds fed the corn-soybean diet. To 28 wk of age, pullets reared on amino-acid-based diets under conditions of elevated protein prices, produced more eggs (P < 0.05) relative to pullets reared on treatments 2 and 4. Results confirmed that pullets can be reared on diets formulated without crude protein specifications, and that substantial use of synthetic amino acids is not detrimental to growth or early reproductive performance. Key words: Pullet growth, amino acid formulation



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