The effect of storage temperature on reducing sugar concentration and the activities of three amylolytic enzymes in tubers of the cultivated potato,Solanum tuberosum L.

1993 ◽  
Vol 36 (2) ◽  
pp. 107-117 ◽  
Author(s):  
J. E. Cottrell ◽  
C. M. Duffus ◽  
L. Paterson ◽  
G. R. Mackay ◽  
M. J. Allison ◽  
...  
2021 ◽  
Vol 5 (2) ◽  
pp. 289-294
Author(s):  
Zeenat Ibrahim Saulawa ◽  
Lawal Nura ◽  
Muntari Bala ◽  
Abdullahi A. Iman

The effectiveness of alkaline hydrogen peroxide as a suitable choice of pretreatment for the conversion of millet husk to reducing sugars using cellulase enzyme for hydrolysis and subsequent ethanol production was determined. The effects of three variables on reducing sugar production from millet husk were determined using one factor at a time (OFAT) method namely; peroxide concentration, pretreatment time and pretreatment temperature. From the results, it was observed that a significant (P<0.05) amount of reducing sugars were lost during pretreatment of millet husk. The untreated group which was only physically pretreated (milled) however yielded a significantly higher (P<0.05) reducing sugar concentration of 10.67mg/ml after enzymatic hydrolysis while the highest reducing sugar concentration of 4.82mg/ml was obtained using 0.375%v/v peroxide concentration for 60minutes at 250C. Therefore, pretreatment of biomass with alkaline hydrogen peroxide may be more suitable for feedstock with high lignin contents than millet husk.


Euphytica ◽  
1991 ◽  
Vol 53 (3) ◽  
pp. 247-253 ◽  
Author(s):  
E. Jacobsen ◽  
M. S. Ramanna ◽  
D. J. Huigen ◽  
Z. Sawor

2002 ◽  
Vol 45 (2-4) ◽  
pp. 215-224 ◽  
Author(s):  
D. S. Douches ◽  
W. W. Kirk ◽  
M. A. Bertram ◽  
J. J. Coombs ◽  
B. A. Niemira

2018 ◽  
Vol 5 (2) ◽  
pp. 86-95
Author(s):  
Ardiansyah Ardiansyah ◽  
Nurlansi Nurlansi ◽  
Rustam Musta

This study aims to determine optimum time of action of the enzyme α-amylase and glucoamylase needed in hydrolyze of starch from waste processed cassava (Manihot esculenta Crantz var. Lahumbu). This research was conducted through three main stages, namely the gelatinization, liquefaction and saccharification. The method was used method are liquefaction and  saccharification. The variation time of the stage liquefaction: 12; 24; 36; 48; 60; and 72 minutes and the saccharification stage are: 9; 18; 27; 36; 45; 54; and 63 hours. The results showed that the optimum time required for stage liquefaction using α-amylase enzyme is 48 minutes on the condition of a temperature of 80 oC with a value of 0.09% amylose levels were measured using UV-Vis spectrophotometer. The optimum time required for saccharification step using a glucoamylase which is 54 hours on the conditions of a temperature of 50oC with the amount of reducing sugar concentration of 9.186 g/L as measured using a UV-Vis spectrophotometer.


2018 ◽  
Vol 156 ◽  
pp. 03006
Author(s):  
Nadiem Anwar ◽  
Iman Mukhaimin ◽  
Mining Harsanti ◽  
Ate Romli

Organic wastes from Swiettenia marcophylla L, Artocarpus heterophyllus L, Mangifera indica L, and Annona muricata L were prepared by grinding into 0.1875, 0.3750, 0.7500 mm of particle size and delignified by 2% NaOH at 80°C for 90 minutes. Acid dilution hydrolysis process with H2SO4 1% was performed at 150°C for 120 minutes in a closed reactor. The effect of particle size and delignification on and reducing sugar concentration were investigated. The result showed (1) leaves that can be used as raw material to produce hydrogen should have 38–49% cellulose and hemicellulose. (2) Reducing sugar concentration increased with particle size reduction and delignification. (3) the best result with the highest reducing sugar concentration was achieved by 0.1875 mm particle size with delignification on Annona muricata L.


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