Bivariate flow-cytometric analysis of regulation of 3T3 cell proliferation

1981 ◽  
Vol 7 (4) ◽  
pp. 318-318
Author(s):  
K. Seuwen ◽  
U. Steiner
Keyword(s):  
Cell Proliferation ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric

Flow cytometric analysis of T cell proliferation in a mixed lymphocyte reaction with dendritic cells

2003 ◽  
Vol 275 (1-2) ◽  
pp. 57-68 ◽  
Author(s):  
Xuan Duc Nguyen ◽  
Hermann Eichler ◽  
Alex Dugrillon ◽  
Christoph Piechaczek ◽  
Michael Braun ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Dendritic Cells ◽  
T Cell ◽  
Mixed Lymphocyte Reaction ◽  
Flow Cytometric Analysis ◽  
T Cell Proliferation ◽  
Flow Cytometric

Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability

Cytometry ◽  
1999 ◽  
Vol 35 (1) ◽  
pp. 64-74 ◽  
Author(s):  
Ingrid Schmid ◽  
John Ferbas ◽  
Christel H. Uittenbogaart ◽  
Janis V. Giorgi
Keyword(s):  
Cell Proliferation ◽  
Flow Cytometric Analysis ◽  
Live Cell ◽  
Flow Cytometric

scholarly journals The expression of CD44, CD90 and CD133 in response to cisplatin in hepatocellular cancer cells

2021 ◽  
Vol 19 (1) ◽  
pp. 18-22
Author(s):  
Yaprak Donmez Cakıl ◽  
◽  
Zeynep Gunes Ozunal ◽  
Damla Gokceoglu Kayalı ◽  
Ranan Gulhan Aktas ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cisplatin Resistance ◽  
Flow Cytometric Analysis ◽  
Hepatocellular Cancer ◽  
Cancer Cell Line ◽  
Therapeutic Strategies ◽  
Resistance To Therapy ◽  
Flow Cytometric ◽  
Dose Dependent Decrease ◽  
Dose Dependent

Introduction. Cancer is a leading cause of mortality. Hepatocellular cancer is one of the malignancies associated with poor outcome and resistance to pharmacotherapy. Cancer stem cells (CSCs) contribute to resistance to therapy and hence lead to the treatment failure of tumors. Aim. This study aims to explore the expression of CSCs in response to cisplatin treatment in HepG2 hepatocellular cancer cell line. Material and methods. Cell proliferation test, CCK-8, was used to evaluate the cell proliferation following cisplatin treatment for 72 hours. The expressions of CSC markers CD44, CD90, and CD133 were assessed by flow cytometric analysis. Results. The results showed a dose-dependent decrease in cell proliferation and increased expression of CSC markers CD44 and CD90 in response to cisplatin. Conclusion. Understanding the roles of CSC markers may point to new targets and therapeutic strategies to predict and overcome cisplatin resistance.

scholarly journals Coix lacryma-jobi var. ma-yuen Stapf sprout extract induces cell cycle arrest and apoptosis in human cervical carcinoma cells

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Eun Suk Son ◽  
Se-Hee Kim ◽  
Young Ock Kim ◽  
Young Eun Lee ◽  
Sun Young Kyung ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Cell Proliferation ◽  
Cell Cycle Arrest ◽  
Hela Cells ◽  
Flow Cytometric Analysis ◽  
B Cell Lymphoma ◽  
Cell Counting ◽  
Cycle Arrest ◽  
Flow Cytometric

Abstract Background Cervical cancer is the second-leading cause of cancer-related mortality in females. Coix lacryma-jobi L. var. ma-yuen (Rom.Caill.) Stapf ex Hook. f. is the most widely recognized medicinal herb for its remedial effects against inflammation, endocrine system dysfunctions, warts, chapped skin, rheumatism, and neuralgia and is also a nourishing food. Methods To investigate the activity of Coix lacryma-jobi sprout extract (CLSE) on cell proliferation in human cervical cancer HeLa cells, we conducted a Cell Counting Kit-8 (CCK-8) assay. Flow-cytometric analysis and western blot analysis were performed to verify the effect of CLSE on the regulation of the cell cycle and apoptosis in HeLa cells. Results We observed that CLSE significantly inhibited cell proliferation. Furthermore, CLSE dose-dependently promoted cell cycle arrest at the sub-G1/ S phase in HeLa cells, as detected by bromodeoxyuridine (BrdU) staining. The cell-cycle-arrest effects of CLSE in HeLa cells were associated with downregulation of cyclin D1 and cyclin-dependent kinases (CDKs) 2, 4, and 6. Moreover, CLSE induced apoptosis, as determined by flow-cytometric analysis and nuclear DNA fragmentation with Annexin V/propidium iodide (PI) and 4′6′-diamidino-2-phenylindole (DAPI) staining. Induction of apoptosis by CLSE was involved in inhibition of the antiapoptotic protein B-cell lymphoma 2 (Bcl-2) and upregulation of the apoptotic proteins p53, cleaved poly (ADP-ribose) polymerase (PARP), cleaved caspase-3, and cleaved caspase-8. Finally, we observed that CLSE inactivated the phosphoinositide 3-kinase (PI3K) and protein kinase B (AKT) pathways. Conclusions CLSE causes cell cycle arrest and apoptotic cell death through inactivation of the PI3K/AKT pathway in HeLa cells, suggesting it is a viable therapeutic agent for cervical cancer owing to its anticancer effects.

Flow cytometric analysis of bromodeoxyuridine-induced inhibition of cell proliferation in the human teratocarcinoma-derived cell line, p3

1989 ◽  
Vol 14 (2) ◽  
pp. 107-114 ◽  
Author(s):  
Suzanne M. Morris ◽  
Lynda J. McGarrity ◽  
Olen E. Domon ◽  
William G. Hinson ◽  
Ralph L. Kodell
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric

scholarly journals Flow cytometric analysis of activation markers on stimulated T cells and their correlation with cell proliferation

Cytometry ◽  
1997 ◽  
Vol 27 (1) ◽  
pp. 71-76 ◽  
Author(s):  
A. Caruso ◽  
S. Licenziati ◽  
M. Corulli ◽  
A.D. Canaris ◽  
M.A. De Francesco ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
T Cells ◽  
Flow Cytometric Analysis ◽  
Activation Markers ◽  
Flow Cytometric

Flow cytometric analysis of cell proliferation dynamics in the B cell compartment of the mouse

1989 ◽  
Vol 1 (4) ◽  
pp. 321-331 ◽  
Author(s):  
Irmgard Förster ◽  
Paulo Vieira ◽  
Klaus Rajewsky
Keyword(s):  
Cell Proliferation ◽  
B Cell ◽  
Flow Cytometric Analysis ◽  
Cell Compartment ◽  
Flow Cytometric
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