Flow cytometric analysis of cell proliferation dynamics in the B cell compartment of the mouse

1989 ◽  
Vol 1 (4) ◽  
pp. 321-331 ◽  
Author(s):  
Irmgard Förster ◽  
Paulo Vieira ◽  
Klaus Rajewsky
Keyword(s):  
Cell Proliferation ◽  
B Cell ◽  
Flow Cytometric Analysis ◽  
Cell Compartment ◽  
Flow Cytometric

Flow cytometric analysis of T cell proliferation in a mixed lymphocyte reaction with dendritic cells

2003 ◽  
Vol 275 (1-2) ◽  
pp. 57-68 ◽  
Author(s):  
Xuan Duc Nguyen ◽  
Hermann Eichler ◽  
Alex Dugrillon ◽  
Christoph Piechaczek ◽  
Michael Braun ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Dendritic Cells ◽  
T Cell ◽  
Mixed Lymphocyte Reaction ◽  
Flow Cytometric Analysis ◽  
T Cell Proliferation ◽  
Flow Cytometric

Roles of Endogenous IL-10 and IL-10-Competent and CD5+ B Cells in Autoimmune Thyroiditis in NOD.H-2h4 Mice

Endocrinology ◽  
2020 ◽  
Vol 161 (4) ◽  
Author(s):  
Jing Qin ◽  
Na Zhao ◽  
Shuo Wang ◽  
Shanshan Liu ◽  
Yongping Liu ◽  
...  
Keyword(s):  
B Cells ◽  
B Cell ◽  
Autoimmune Thyroiditis ◽  
Flow Cytometric Analysis ◽  
Cell Group ◽  
Autoantibody Production ◽  
Flow Cytometric ◽  
Cell Groups ◽  
Regulatory Capacity ◽  
Anti Inflammatory Cytokine

Abstract Interleukin (IL)-10 is a highly important anti-inflammatory cytokine in the immune system. CD1dhi and CD5+ B cells are both traditionally defined IL-10-secreting B cells. In recent years, a B cell group with combined markers of CD1dhi and CD5+ has been widely studied as it has been reported to suppress autoimmunity in mouse models of autoimmune diseases through IL-10 mechanisms. From the perspective of origination, CD1dhi and CD5+ B cells are developed from different B cell lineages. Whether the regulatory capacity of these 2 B cell groups is consistent with their ability to secrete IL-10 has not been determined. In this study, we generated IL-10 knockout NOD.H-2h4 mice to investigate the function of endogenous IL-10 in autoimmune thyroiditis and conducted adoptive transfer experiments to explore the respective roles of CD5+ and CD1dhi B cells. In our results, the IL-10–/– NOD.H-2h4 mice developed thyroiditis, similar to wild-type NOD.H-2h4 mice. The CD5+ B cells were more capable of secreting IL-10 than CD1dhi B cells in flow cytometric analysis, but the CD1dhi B cells showed more suppressive effects on thyroiditis development and autoantibody production, as well as Th17 cell response. In conclusion, endogenous IL-10 does not play an important role in autoimmune thyroiditis. CD1dhi B cells may play regulatory roles through mechanisms other than secreting IL-10.

Flow cytometric analysis of live cell proliferation and phenotype in populations with low viability

Cytometry ◽  
1999 ◽  
Vol 35 (1) ◽  
pp. 64-74 ◽  
Author(s):  
Ingrid Schmid ◽  
John Ferbas ◽  
Christel H. Uittenbogaart ◽  
Janis V. Giorgi
Keyword(s):  
Cell Proliferation ◽  
Flow Cytometric Analysis ◽  
Live Cell ◽  
Flow Cytometric

scholarly journals Myeloid cell dynamics correlate with clinical outcomes of severe coronavirus disease 2019

2020 ◽  
Author(s):  
Tomohiro Takano ◽  
Takayuki Matsumura ◽  
Yu Adachi ◽  
Kazutaka Terahara ◽  
Saya Moriyama ◽  
...  
Keyword(s):  
Clinical Outcome ◽  
Flow Cytometric Analysis ◽  
Myeloid Cell ◽  
Suppressor Cell ◽  
Recovery Phase ◽  
Cell Compartment ◽  
Cell Dynamics ◽  
Flow Cytometric ◽  
Myeloid Derived Suppressor Cell ◽  
Plasma Cytokines

Abstract An expanded myeloid cell compartment is a hallmark of severe coronavirus disease 2019 (COVID-19); however, it remains unclear whether myeloid cells are beneficial or detrimental to the clinical outcome. Here, we tracked cellular dynamics of myeloid-derived suppressor cell (MDSC) subsets and examined whether any of them correlate with disease severity and prognosis by flow cytometric analysis of blood samples from COVID-19 patients. We observed that polymorphonuclear (PMN)-MDSCs, rather than other MDSC subsets, transiently expanded in severe cases but not in mild or moderate cases. Notably, this subset was selectively expanded in survivors of severe cases and diminished during recovery. Analysis of plasma cytokines/chemokines revealed that interleukin-8 increased prior to PMN-MDSC expansion in survivors and returned to basal levels during the recovery phase. In contrast, interleukin-6 and interferon--induced protein 10 were abundantly induced in non-survivors, suggesting possible downstream targets for the immunosuppressive effects of the MDSC subset. Our data indicate that increased cellularity of PMN-MDSCs might be beneficial for the clinical outcome and could be useful as a possible predictor of prognosis in cases of severe COVID-19.

scholarly journals Flow Cytometric Analysis Revealed a Significant Accumulation of Terminally Differentiated T cell Subtypes in the Circulating Lymphocytes of Cytomegalovirus (CMV) Positive Follicular Lymphoma Patients

2021 ◽  
pp. 1-9
Author(s):  
Lugos MD ◽  
◽  
Dangana A ◽  
Ntuhun BD ◽  
Oluwatayo BO ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Follicular Lymphoma ◽  
B Cell ◽  
Flow Cytometric Analysis ◽  
B Cell Lymphoma ◽  
T Cell Subsets ◽  
Cmv Infection ◽  
Flow Cytometric ◽  
The Impact

Follicular lymphoma (FL), a non-Hodgkin lymphoma, is an indolent cancer of the B cell lineage that runs a chronic deterioration course that can result in multiple treatment episodes leading to resistance and possible transformation to diffuse large B cell lymphoma. Cytomegalovirus (CMV) reactivation during chemotherapy or after an organ or hematopoietic stem cell transplantation is a major cause of morbidity and mortality. This study tests the hypothesis that some of the heterogeneity of FL might result from chronic infection with Cytomegalovirus (CMV). This research was intended to appraise the impact of CMV infection on the subtypes of T cells in follicular lymphoma patients. We accessed stored peripheral blood mononuclear cells (PMBCs) from patients of known CMV serostatus recruited into an FL clinical trial. We undertook a multicolour flow cytometric analysis of the PBMCs and compared the number of lymphocyte subtypes of CMV-positive and CMV-negative FL patients. Data showed a significant increase in the quantity of terminally differentiated (TEMRA) T cell subsets, including EM3-CD8 (P=0.005), EM3-CD4 (P=0.018), E-CD4 (P=0.029), E-CD8 (P=0.033) and pE2-CD4 (P=0.046) phenotypes, as well as increased NKT cells (P=0.031) among CMV-positive patients compared to the negative group. Our findings support the hypothesis that recurrent infections characterise CMV infection in FL due to accelerated immune senescence and the accumulation of exhausted T cells. Based on the data, a case could be argued for the routine application of CMV screening in FL before treatment with chemo-immunotherapy to implement enhanced infection surveillance in CMV-positive patients. These discoveries can eventually help improve the treatment approaches in the management of FL toward a combinatorial viewpoint for direct cytotoxic and indirect immunomodulatory outlook

scholarly journals The expression of CD44, CD90 and CD133 in response to cisplatin in hepatocellular cancer cells

2021 ◽  
Vol 19 (1) ◽  
pp. 18-22
Author(s):  
Yaprak Donmez Cakıl ◽  
◽  
Zeynep Gunes Ozunal ◽  
Damla Gokceoglu Kayalı ◽  
Ranan Gulhan Aktas ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cisplatin Resistance ◽  
Flow Cytometric Analysis ◽  
Hepatocellular Cancer ◽  
Cancer Cell Line ◽  
Therapeutic Strategies ◽  
Resistance To Therapy ◽  
Flow Cytometric ◽  
Dose Dependent Decrease ◽  
Dose Dependent

Introduction. Cancer is a leading cause of mortality. Hepatocellular cancer is one of the malignancies associated with poor outcome and resistance to pharmacotherapy. Cancer stem cells (CSCs) contribute to resistance to therapy and hence lead to the treatment failure of tumors. Aim. This study aims to explore the expression of CSCs in response to cisplatin treatment in HepG2 hepatocellular cancer cell line. Material and methods. Cell proliferation test, CCK-8, was used to evaluate the cell proliferation following cisplatin treatment for 72 hours. The expressions of CSC markers CD44, CD90, and CD133 were assessed by flow cytometric analysis. Results. The results showed a dose-dependent decrease in cell proliferation and increased expression of CSC markers CD44 and CD90 in response to cisplatin. Conclusion. Understanding the roles of CSC markers may point to new targets and therapeutic strategies to predict and overcome cisplatin resistance.

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