Effect of the physical properties of Lentinula edodes bedlogs on fruiting body production

Mycoscience ◽  
1998 ◽  
Vol 39 (2) ◽  
pp. 217-219 ◽  
Author(s):  
Keisuke Tokimoto ◽  
Masaki Fukuda ◽  
Masatomo Tsuboi
Keyword(s):  
Physical Properties ◽  
Fruiting Body ◽  
Lentinula Edodes ◽  
Body Production

scholarly journals Molecular Cloning, Characterization, and Differential Expression of a Glucoamylase Gene from the Basidiomycetous Fungus Lentinula edodes

2000 ◽  
Vol 66 (6) ◽  
pp. 2531-2535 ◽  
Author(s):  
J. Zhao ◽  
Y. H. Chen ◽  
H. S. Kwan
Keyword(s):  
Gene Expression ◽  
Fruiting Body ◽  
Lentinula Edodes ◽  
Catalytic Domain ◽  
Coding Region ◽  
Fruiting Body Formation ◽  
Mycelium Growth ◽  
Body Formation ◽  
Basidiomycetous Fungus ◽  
Glucoamylase Gene

ABSTRACT The complete nucleotide sequence of putative glucoamylase genegla1 from the basidiomycetous fungus Lentinula edodes strain L54 is reported. The coding region of the genomic glucoamylase sequence, which is preceded by eukaryotic promoter elements CAAT and TATA, spans 2,076 bp. The gla1 gene sequence codes for a putative polypeptide of 571 amino acids and is interrupted by seven introns. The open reading frame sequence of thegla1 gene shows strong homology with those of other fungal glucoamylase genes and encodes a protein with an N-terminal catalytic domain and a C-terminal starch-binding domain. The similarity between the Gla1 protein and other fungal glucoamylases is from 45 to 61%, with the region of highest conservation found in catalytic domains and starch-binding domains. We compared the kinetics of glucoamylase activity and levels of gene expression in L. edodes strain L54 grown on different carbon sources (glucose, starch, cellulose, and potato extract) and in various developmental stages (mycelium growth, primordium appearance, and fruiting body formation). Quantitative reverse transcription PCR utilizing pairs of primers specific forgla1 gene expression shows that expression ofgla1 was induced by starch and increased during the process of fruiting body formation, which indicates that glucoamylases may play an important role in the morphogenesis of the basidiomycetous fungus.

scholarly journals A Novel Gene, Le-Dd 10, Is Involved in Fruiting Body Formation of Lentinula Edodes

2022 ◽  
Author(s):  
Akihiro Kishikawa ◽  
Satoshi Hamada ◽  
Ichiro Kamei ◽  
Yosuke Fujimoto ◽  
Kazuhiro Miyazaki ◽  
...  
Keyword(s):  
Cdna Library ◽  
Fruiting Body ◽  
Lentinula Edodes ◽  
Polyclonal Antibodies ◽  
Single Strand Conformation Polymorphism ◽  
Positive Control ◽  
Culture Conditions ◽  
Negative Control ◽  
Polymorphism Analysis ◽  
Fruiting Body Formation

Abstract The cDNA library prepared from Lentinula edodes, Hokken 600 (H600), primordia was screened by using cDNA expressed specifically in Dictyostelium discoideum prestalk as a probe. Twenty-one clones, Le-Dd 1~21, were isolated from the L. edodes primordia cDNA library. Functional analysis of each gene was carried out by transformation into protoplast cells from L. edodes Mori 252 (M252) mycelia with the overexpression vector pLG-RasF1 of each gene because M252 protoplast cells were transformed with 11-fold higher efficiency than H600 cells. Transformants with the overexpression vector of Le-Dd10 formed a fruiting body at almost the same time as H600, a positive control, although M252, a negative control, did not form a fruiting body under culture conditions. This suggested that Le-Dd10 is involved in the formation of fruiting bodies. Single-strand conformation polymorphism analysis revealed that Le-Dd10 is located on No. 4 linkage group of L. edodes. The properties of Le-Dd10 products were investigated by Western blotting analysis using polyclonal antibodies against GST:Le-Dd10 fusion proteins. As a result, 56-kDa, 27-kDa, and 14-kDa protein bands appeared in primordial and fruiting body stages, although the expected molecular weight of the Le-Dd10 product was 50 kDa.

scholarly journals Long term observation of mycorrhizal status and above-ground fungi fruiting body production in oak forest

Dendrobiology ◽  
2012 ◽  
Vol 69 ◽  
pp. 99-110 ◽  
Author(s):  
Vítězslava Pešková ◽  
Jaroslav Landa ◽  
Roman Modlinger
Keyword(s):  
Fruiting Body ◽  
Oak Forest ◽  
Mycorrhizal Status ◽  
Long Term Observation ◽  
Body Production

scholarly journals Pertumbuhan dan Produksi Jamur Lentinus sajor-caju isolat LSC9 pada Media Serbuk Gergajian Kayu Sengon (Paraserianthes falcataria) dan Tandan Kosong Kelapa Sawit

2016 ◽  
Vol 1 (2) ◽  
pp. 41-46
Author(s):  
HENNY SULISTANY ◽  
LISDAR IDWAN SUDIRMAN
Keyword(s):  
Oil Palm ◽  
Fruiting Body ◽  
Growth And Development ◽  
Biological Efficiency ◽  
Mushroom Cultivation ◽  
Paraserianthes Falcataria ◽  
Alternative Substrates ◽  
Generative Phase ◽  
Body Production ◽  
Better Than

Paraserianthes falcataria sawdust (SGS) and oil palm empty fruit bunch (TKKS) are by-product of forestry and oil palm industries. SGS is commonly substrates for mushroom cultivation. TKKS is expected to be an alternative substrates for mushroom cultivation besides SGS. This study was conducted to determine the growth and fruiting body production of Lentinus sajor-caju LSC9 isolate on SGS, TKKS and mixtures of both substrates (C) with proportion 1:1 respectively. Each substrates were added with 15% rice bran, 1.5% gypsum and 1.5% CaCO3 with a total weight of 500 g/bag. The result showed that the growth and fruiting body production of Lentinus sajor-caju LSC9 isolate on SGS was better than TKKS and C substrates with biological efficiency on SGS substrates (50.88%) higher than TKKS substrates (34.42%) and C substrates (29.51%), with vegetative phase (16 days), generative phase (100 days) and growth and development phase (115 days) on TKKS substrates were shorter than SGS and C substrates. The greatest pileus number found on SGS substrates (12 pieces), while the largest pileus diameter found on C substrates (10.17 cm). Nevertheless, TKKS can be used as alternative substrates for fruiting body production of Lentinus sajor-caju LSC9 isolate. 

scholarly journals Selection of Reference Genes for qRT-PCR Analysis in Lentinula edodes after Hot-Air Drying

Molecules ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 136 ◽  
Author(s):  
Shuangshuang Gao ◽  
Gangzheng Wang ◽  
Zhicheng Huang ◽  
Xiaoyu Lei ◽  
Yinbing Bian ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Reference Genes ◽  
Lentinula Edodes ◽  
Sulfur Compounds ◽  
Pcr Analysis ◽  
Expression Stability ◽  
Hot Air Drying ◽  
Air Drying ◽  
Qrt Pcr ◽  
Hot Air

Volatile sulfur compounds gradually develop in Lentinula edodes after hot-air drying, and many genes are involved in the generation of these sulfur compounds. The expression stability of reference genes may vary in a particular experimental treatment when analyzing their expressions by quantitative real-time polymerase chain reaction (qRT-PCR). In this study, the expression profile of 17 candidate genes was assessed in L. edodes under treatment at 50 °C for 0, 1, 2, and 3 h, and the expression stability of each reference gene was analyzed by three statistical algorithms, including geNorm, NormFinder, and BestKeeper. Results indicated that the two optimal reference genes for mycelium and fruiting body were CAC and DAHP as well as CAC and NUP, respectively. Additionally, CAC and DAHP were found to be the two most stable reference genes across the mycelium and fruiting body set. Our results will provide a genetic foundation for further research on the metabolism genes of sulfur compounds in L. edodes.

scholarly journals Cmfhp Gene Mediates Fruiting Body Development and Carotenoid Production in Cordyceps militaris

Biomolecules ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 410 ◽  
Author(s):  
Hai-Wei Lou ◽  
Yu Zhao ◽  
Bai-Xiong Chen ◽  
Ying-Hao Yu ◽  
Hong-Biao Tang ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Transformation Method ◽  
Cordyceps Militaris ◽  
Carotenoid Content ◽  
Fruiting Bodies ◽  
Developmental Mechanism ◽  
Targeted Gene Deletion ◽  
Body Development ◽  
Gene Expression Cassette ◽  
Body Production

Cordyceps militaris fruiting bodies contain a variety of bioactive components that are beneficial to the human body. However, the low yield of fruiting bodies and the low carotenoid content in C. militaris have seriously hindered the development of the C. militaris industry. To elucidate the developmental mechanism of the fruiting bodies of C. militaris and the biosynthesis mechanism of carotenoids, the function of the flavohemoprotein-like Cmfhp gene of C. militaris was identified for the first time. The Cmfhp gene was knocked out by the split-marker method, and the targeted gene deletion mutant ΔCmfhp was obtained. An increased nitric oxide (NO) content, no fruiting body production, decreased carotenoid content, and reduced conidial production were found in the mutant ΔCmfhp. These characteristics were restored when the Cmfhp gene expression cassette was complemented into the ΔCmfhp strain by the Agrobacterium tumefaciens-mediated transformation method. Nonetheless, the Cmfhp gene had no significant effect on the mycelial growth rate of C. militaris. These results indicated that the Cmfhp gene regulated the biosynthesis of NO and carotenoids, the development of fruiting bodies, and the formation of conidia. These findings potentially pave the way to reveal the developmental mechanism of fruiting bodies and the biosynthesis mechanism of carotenoids in C. militaris.

scholarly journals Expression Profile of Laccase Gene Family in White-Rot Basidiomycete Lentinula edodes under Different Environmental Stresses

Genes ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1045
Author(s):  
Lianlian Yan ◽  
Ruiping Xu ◽  
Yinbing Bian ◽  
Hongxian Li ◽  
Yan Zhou
Keyword(s):  
Gene Family ◽  
Fruiting Body ◽  
Lentinula Edodes ◽  
Expression Profiles ◽  
Draft Genome ◽  
White Rot ◽  
Laccase Gene ◽  
Fruiting Body Formation ◽  
Body Formation ◽  
Laccase Genes

Laccases belong to ligninolytic enzymes and play important roles in various biological processes of filamentous fungi, including fruiting-body formation and lignin degradation. The process of fruiting-body development in Lentinula edodes is complex and is greatly affected by environmental conditions. In this paper, 14 multicopper oxidase-encoding (laccase) genes were analyzed in the draft genome sequence of L. edodes strain W1-26, followed by a search of multiple stress-related Cis-elements in the promoter region of these laccase genes, and then a transcription profile analysis of 14 laccase genes (Lelcc) under the conditions of different carbon sources, temperatures, and photoperiods. All laccase genes were significantly regulated by varying carbon source materials. The expression of only two laccase genes (Lelcc5 and Lelcc6) was induced by sodium-lignosulphonate and the expression of most laccase genes was specifically upregulated in glucose medium. Under different temperature conditions, the expression levels of most laccase genes decreased at 39 °C and transcription was significantly increased for Lelcc1, Lelcc4, Lelcc5, Lelcc9, Lelcc12, Lelcc13, and Lelcc14 after induction for 24 h at 10 °C, indicating their involvement in primordium differentiation. Tyrosinase, which is involved in melanin synthesis, was clustered with the same group as Lelcc4 and Lelcc7 in all the different photoperiod treatments. Meanwhile, five laccase genes (Lelcc8, Lelcc9, Lelcc12, Lelcc13, and Lelcc14) showed similar expression profiles to that of two blue light receptor genes (LephrA and LephrB) in the 12 h light/12 h dark treatment, suggesting the involvement of laccase genes in the adaptation process of L. edodes to the changing environment and fruiting-body formation. This study contributes to our understanding of the function of the different Lelcc genes and facilitates the screening of key genes from the laccase gene family for further functional research.

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