scholarly journals A Novel Gene, Le-Dd 10, Is Involved in Fruiting Body Formation of Lentinula Edodes

2022 ◽  
Author(s):  
Akihiro Kishikawa ◽  
Satoshi Hamada ◽  
Ichiro Kamei ◽  
Yosuke Fujimoto ◽  
Kazuhiro Miyazaki ◽  
...  
Keyword(s):  
Cdna Library ◽  
Fruiting Body ◽  
Lentinula Edodes ◽  
Polyclonal Antibodies ◽  
Single Strand Conformation Polymorphism ◽  
Positive Control ◽  
Culture Conditions ◽  
Negative Control ◽  
Polymorphism Analysis ◽  
Fruiting Body Formation

Abstract The cDNA library prepared from Lentinula edodes, Hokken 600 (H600), primordia was screened by using cDNA expressed specifically in Dictyostelium discoideum prestalk as a probe. Twenty-one clones, Le-Dd 1~21, were isolated from the L. edodes primordia cDNA library. Functional analysis of each gene was carried out by transformation into protoplast cells from L. edodes Mori 252 (M252) mycelia with the overexpression vector pLG-RasF1 of each gene because M252 protoplast cells were transformed with 11-fold higher efficiency than H600 cells. Transformants with the overexpression vector of Le-Dd10 formed a fruiting body at almost the same time as H600, a positive control, although M252, a negative control, did not form a fruiting body under culture conditions. This suggested that Le-Dd10 is involved in the formation of fruiting bodies. Single-strand conformation polymorphism analysis revealed that Le-Dd10 is located on No. 4 linkage group of L. edodes. The properties of Le-Dd10 products were investigated by Western blotting analysis using polyclonal antibodies against GST:Le-Dd10 fusion proteins. As a result, 56-kDa, 27-kDa, and 14-kDa protein bands appeared in primordial and fruiting body stages, although the expected molecular weight of the Le-Dd10 product was 50 kDa.

scholarly journals Molecular Cloning, Characterization, and Differential Expression of a Glucoamylase Gene from the Basidiomycetous Fungus Lentinula edodes

2000 ◽  
Vol 66 (6) ◽  
pp. 2531-2535 ◽  
Author(s):  
J. Zhao ◽  
Y. H. Chen ◽  
H. S. Kwan
Keyword(s):  
Gene Expression ◽  
Fruiting Body ◽  
Lentinula Edodes ◽  
Catalytic Domain ◽  
Coding Region ◽  
Fruiting Body Formation ◽  
Mycelium Growth ◽  
Body Formation ◽  
Basidiomycetous Fungus ◽  
Glucoamylase Gene

ABSTRACT The complete nucleotide sequence of putative glucoamylase genegla1 from the basidiomycetous fungus Lentinula edodes strain L54 is reported. The coding region of the genomic glucoamylase sequence, which is preceded by eukaryotic promoter elements CAAT and TATA, spans 2,076 bp. The gla1 gene sequence codes for a putative polypeptide of 571 amino acids and is interrupted by seven introns. The open reading frame sequence of thegla1 gene shows strong homology with those of other fungal glucoamylase genes and encodes a protein with an N-terminal catalytic domain and a C-terminal starch-binding domain. The similarity between the Gla1 protein and other fungal glucoamylases is from 45 to 61%, with the region of highest conservation found in catalytic domains and starch-binding domains. We compared the kinetics of glucoamylase activity and levels of gene expression in L. edodes strain L54 grown on different carbon sources (glucose, starch, cellulose, and potato extract) and in various developmental stages (mycelium growth, primordium appearance, and fruiting body formation). Quantitative reverse transcription PCR utilizing pairs of primers specific forgla1 gene expression shows that expression ofgla1 was induced by starch and increased during the process of fruiting body formation, which indicates that glucoamylases may play an important role in the morphogenesis of the basidiomycetous fungus.

scholarly journals Probiotic modulation of the gut bacterial community of juvenile Litopenaeus vannamei challenged with Vibrio parahaemolyticus CAIM 170

2017 ◽  
Vol 43 (4) ◽  
pp. 766-775
Author(s):  
Irasema E. Luis-Villaseñor ◽  
Domenico Voltolina ◽  
Bruno Gomez-Gil ◽  
Felipe Ascencio ◽  
Ángel I. Campa-Córdova ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Litopenaeus Vannamei ◽  
Vibrio Parahaemolyticus ◽  
Single Strand Conformation Polymorphism ◽  
Positive Control ◽  
Negative Control ◽  
Rrna Gene ◽  
Protective Effects ◽  
Probiotic Treatment ◽  
Significant Difference

The protective effects of two probiotic mixtures was studied using the fingerprints of the bacterial community of Litopenaeus vannamei juveniles exposed to probiotics and challenged with Vibrio parahaemolyticus CAIM 170. Fingerprints were constructed using 16S rRNA gene and the PCR-SSCP (Single strand conformation polymorphism) technique, and the probiotics used were an experimental Bacillus mixture (Bacillus tequilensis YC5-2 + B. endophyticus C2-2 and YC3-B) and the commercial probiotic Alibio. The DNA for PCR-SSCP analyses was extracted directly from the guts of shrimps treated for 20 days with the probiotics and injected with 2.5×105 CFU g-1 of V. parahaemolyticus one week after suspension of the probiotic treatment. Untreated shrimps served as positive (injected with V. parahaemolyticus) and negative (not injected) controls Analysis of the bacterial community carried out after inoculation and 12 and 48 h later confirmed that V. parahaemolyticus was present in shrimps of the positive control , but not in the negative control or treated with the probiotic mixtures. A significant difference in the diversity of the bacterial community was observed between times after infection. The band patterns in 0-12 h were clustered into a different group from that determined after 48 h, and suggested that during bacterial infection the guts of whiteleg shrimp were dominated by gamma proteobacteria represented by Vibrio sp. and Photobacterium sp. Our results indicate that the experimental and the commercial mixtures are suitable to modulate the bacterial community of L. vannamei and could be used as a probiotic to control vibriosis in juvenile shrimp.

The Optimal Culture Conditions Affecting the Mycelial Growth and Fruiting Body Formation ofPaecilomyces fumosoroseus

Mycobiology ◽  
2003 ◽  
Vol 31 (4) ◽  
pp. 214 ◽  
Author(s):  
Sung Mi Shim ◽  
Kyung Rim Lee ◽  
Seong Hwan Kim ◽  
Kyung Hoan Im ◽  
Jung Wan Kim ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Mycelial Growth ◽  
Culture Conditions ◽  
Fruiting Body Formation ◽  
Body Formation

scholarly journals Two Cases of Natural Infection of Dengue-2 Virus in Bats in the Colombian Caribbean

2021 ◽  
Vol 6 (1) ◽  
pp. 35
Author(s):  
Alfonso Calderón ◽  
Camilo Guzmán ◽  
Teresa Oviedo-Socarras ◽  
Salim Mattar ◽  
Virginia Rodríguez ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Viral Infections ◽  
Polyclonal Antibodies ◽  
Natural Infection ◽  
Immunohistochemical Analysis ◽  
Positive Control ◽  
Negative Control ◽  
Sodium Pentobarbital ◽  
Rt Pcr ◽  
Cross Sectional

Dengue, a mosquito-borne zoonotic disease, is the most common vector-borne disease in tropical and subtropical areas. In this study, we aim to demonstrate biological evidence of dengue virus infection in bats. A cross-sectional study was carried out in the departments of Cordoba and Sucre, Colombia. A total of 286 bats were captured following the ethical protocols of animal experimentation. The specimens were identified and euthanized using a pharmacological treatment with atropine, acepromazine and sodium pentobarbital. Duplicate samples of brain, heart, lung, spleen, liver, and kidney were collected with one set stored in Trizol and the other stored in 10% buffered formalin for histopathological and immunohistochemical analysis using polyclonal antibodies. Brain samples from lactating mice with an intracranial inoculation of DENV-2 were used as a positive control. As a negative control, lactating mouse brains without inoculation and bats brains negative for RT-PCR were included. Tissue sections from each specimen of bat without conjugate were used as staining control. In a specimen of Carollia perspicillata captured in Ayapel (Cordoba) and Phylostomus discolor captured in San Carlos (Cordoba), dengue virus was detected, and sequences were matched to DENV serotype 2. In bats RT-PCR positive for dengue, lesions compatible with viral infections, and the presence of antigens in tissues were observed. Molecular findings, pathological lesions, and detection of antigens in tissues could demonstrate viral DENV-2 replication and may correspond to natural infection in bats. Additional studies are needed to elucidate the exact role of these species in dengue epidemics.

scholarly journals Expression Profile of Laccase Gene Family in White-Rot Basidiomycete Lentinula edodes under Different Environmental Stresses

Genes ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1045
Author(s):  
Lianlian Yan ◽  
Ruiping Xu ◽  
Yinbing Bian ◽  
Hongxian Li ◽  
Yan Zhou
Keyword(s):  
Gene Family ◽  
Fruiting Body ◽  
Lentinula Edodes ◽  
Expression Profiles ◽  
Draft Genome ◽  
White Rot ◽  
Laccase Gene ◽  
Fruiting Body Formation ◽  
Body Formation ◽  
Laccase Genes

Laccases belong to ligninolytic enzymes and play important roles in various biological processes of filamentous fungi, including fruiting-body formation and lignin degradation. The process of fruiting-body development in Lentinula edodes is complex and is greatly affected by environmental conditions. In this paper, 14 multicopper oxidase-encoding (laccase) genes were analyzed in the draft genome sequence of L. edodes strain W1-26, followed by a search of multiple stress-related Cis-elements in the promoter region of these laccase genes, and then a transcription profile analysis of 14 laccase genes (Lelcc) under the conditions of different carbon sources, temperatures, and photoperiods. All laccase genes were significantly regulated by varying carbon source materials. The expression of only two laccase genes (Lelcc5 and Lelcc6) was induced by sodium-lignosulphonate and the expression of most laccase genes was specifically upregulated in glucose medium. Under different temperature conditions, the expression levels of most laccase genes decreased at 39 °C and transcription was significantly increased for Lelcc1, Lelcc4, Lelcc5, Lelcc9, Lelcc12, Lelcc13, and Lelcc14 after induction for 24 h at 10 °C, indicating their involvement in primordium differentiation. Tyrosinase, which is involved in melanin synthesis, was clustered with the same group as Lelcc4 and Lelcc7 in all the different photoperiod treatments. Meanwhile, five laccase genes (Lelcc8, Lelcc9, Lelcc12, Lelcc13, and Lelcc14) showed similar expression profiles to that of two blue light receptor genes (LephrA and LephrB) in the 12 h light/12 h dark treatment, suggesting the involvement of laccase genes in the adaptation process of L. edodes to the changing environment and fruiting-body formation. This study contributes to our understanding of the function of the different Lelcc genes and facilitates the screening of key genes from the laccase gene family for further functional research.

scholarly journals Mapping of genes abundantly expressed during fruiting body formation of Lentinula edodes

2010 ◽  
Vol 60 (1) ◽  
pp. 81-86 ◽  
Author(s):  
Kazuhiro Miyazaki ◽  
Miho Sakai ◽  
Yasumasa Miyazaki
Keyword(s):  
Fruiting Body ◽  
Lentinula Edodes ◽  
Fruiting Body Formation ◽  
Body Formation

Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

2017 ◽  
Vol 9 (2) ◽  
pp. 71
Author(s):  
Nurhasanah Nurhasanah ◽  
Fauzia Andrini ◽  
Yulis Hamidy
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Allium Sativum ◽  
Fungicidal Activity ◽  
Positive Control ◽  
Negative Control ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

Sign in / Sign up

Export Citation Format

Share Document