A study on pathogens of Chinese prawn (Penaeus Chinensis) virus diseases

1995 ◽  
Vol 13 (3) ◽  
pp. 284-288
Author(s):  
Sun Xiu-qin ◽  
Zhang Jin-xing



Aquaculture ◽  
1994 ◽  
Vol 127 (1) ◽  
pp. 29-40 ◽  
Author(s):  
X.L. Xu ◽  
W.J. Ji ◽  
J.D. Castell ◽  
R.K. O'Dor


2010 ◽  
Vol 60 (11) ◽  
pp. 2517-2523 ◽  
Author(s):  
Yan Wang ◽  
Xiao-Hua Zhang ◽  
Min Yu ◽  
Hong Wang ◽  
Brian Austin

A culture (designated strain HHS02T) was isolated from Chinese prawn (Penaeus chinensis, O'sbeck) and determined to be a member of the genus Vibrio. Strain HHS02T comprised slightly curved, rod-shaped, non-endospore-forming, Gram-negative, catalase-negative, oxidase-positive, O/129-sensitive and facultatively anaerobic cells that were motile by means of a single polar flagellum. Growth of strain HHS02T occurred in 0.5–7 % (w/v) NaCl [optimally in 1–3 % (w/v) NaCl] and between pH 7.0 and 10.0 (optimally at pH 8.0–9.0). The strain showed growth between 16 and 30 °C (optimum 20 °C). Analysis using the 16S rRNA, gapA, gyrB, mreB, pyrH, recA and topA gene sequences of the novel isolate revealed that the organism belonged to the genus Vibrio, with ∼98, 98, 90, 88, 92, 89 and 83 % sequence similarity, respectively, with representatives of the genus Vibrio. DNA–DNA hybridization experiments indicated that the novel strain was distinct from recognized species of the genus Vibrio. The major fatty acid components were summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH, 38.7 %), C16 : 0 (22.9 %) and C18 : 1 ω7c (12.5 %). The G+C content of the genomic DNA was 44.4 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, it is concluded that strain HHS02T should be classified as a novel species of the genus Vibrio, for which the name Vibrio atypicus sp. nov. is proposed. The type strain is HHS02T (=CGMCC 1.8461T=LMG 24781T).



Aquaculture ◽  
1993 ◽  
Vol 118 (3-4) ◽  
pp. 277-285 ◽  
Author(s):  
Xueling Xu ◽  
Wenjuan Ji ◽  
John D. Castell ◽  
Ron O'Dor


Author(s):  
O. E. Bradfute

Electron microscopy is frequently used in preliminary diagnosis of plant virus diseases by surveying negatively stained preparations of crude extracts of leaf samples. A major limitation of this method is the time required to survey grids when the concentration of virus particles (VPs) is low. A rapid survey of grids for VPs is reported here; the method employs a low magnification, out-of-focus Search Mode similar to that used for low dose electron microscopy of radiation sensitive specimens. A higher magnification, in-focus Confirm Mode is used to photograph or confirm the detection of VPs. Setting up the Search Mode by obtaining an out-of-focus image of the specimen in diffraction (K. H. Downing and W. Chiu, private communications) and pre-aligning the image in Search Mode with the image in Confirm Mode facilitates rapid switching between Modes.



1953 ◽  
Vol 23 (1) ◽  
pp. 121
Keyword(s):  


1950 ◽  
Vol 34 (6) ◽  
pp. 1637-1652
Author(s):  
Thomas M. Durant ◽  
Charles R. Shuman




1963 ◽  
Vol 02 (02) ◽  
pp. 49-51 ◽  
Author(s):  
K. Knapp ◽  
W. Lenz

SummaryIn 1961 an increase in certain congenital malformations was noticed in various parts of the Federal Republic of Germany. From the outset, it seemed very probable that a single cause was responsible, since, although these malformations varied, they appeared to belong to one and the same syndrome. For general reasons, such causes as radioactivity, contraceptives, attempted abortions or virus diseases did not provide an acceptable explanation. The epidemiology was, however, suggestive of some chemical substance taken orally.After thalidomide had been indicated in several histories, suspicion of that drug was aroused and, within one week, intake of it could be proved or was found to be very likely in 17 out of 20 cases, while in the remaining cases it could not be excluded. Though this first result was highly significant, we were at first reluctant to incriminate thalidomide definitely, because there was still some doubt about the reliability of the histories. But within a few weeks, the thalidomide aetiology received support from many sources. Specific studies on limited material are sometimes superior to extensive, broadly planned investigations.



2020 ◽  
Vol 1 (1) ◽  
pp. 51-57
Author(s):  
Meghnath Dhimal ◽  
Tamanna Neupane ◽  
Samir Kumar Adhikari ◽  
Pradip Gyanwali

We are facing global pandemic of novel corona virus diseases COVID-19 which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This paper is aimed to assess trend of COVID-19 cases and health sector response in Nepal. We reviewed WHO databases to observe the global trends and epidemiology of COVID-19 as well as daily situation updated reports of Health Emergency and Operation Centre (HEOC), guidelines, national and international government documents. The first case of COVID was reported in Nepal on 23 January 2020 and number of cases reached 454 on 21 May 2020. In order to address the increasing number of cases of COVID-19, Government of Nepal is adopting various preventive measures like extending lockdown period, setting up quarantine and isolation facilities, sealing borders, suspending flights, closing public places etc. There is need of joint effort by individuals, communities and government to prevent the further spread and flatten epidemic curve in Nepal.



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