Baicalin production in transformed hairy root clones ofScutellaria baicalensis

2006 ◽  
Vol 11 (2) ◽  
pp. 105-109 ◽  
Author(s):  
Sung Jin Hwang
Keyword(s):  
Hairy Root ◽  
Transformed Hairy Root ◽  
Hairy Root Clones

scholarly journals Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1610
Author(s):  
Branka Vinterhalter ◽  
Nevena Banjac ◽  
Dragan Vinterhalter ◽  
Dijana Krstić-Milošević
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Bubble Column ◽  
Growth Parameters ◽  
Growth Index ◽  
Dry Weight ◽  
Growth Conditions ◽  
Good Growth ◽  
Gentiana Dinarica ◽  
Hairy Root Clones

The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.

scholarly journals Response of Soybean Pathogens to Glyceollin

2010 ◽  
Vol 100 (9) ◽  
pp. 897-903 ◽  
Author(s):  
Anatoly V. Lygin ◽  
Curtis B. Hill ◽  
Olga V. Zernova ◽  
Laura Crull ◽  
Jack M. Widholm ◽  
...  
Keyword(s):  
Hairy Root ◽  
Degradation Products ◽  
Macrophomina Phaseolina ◽  
Phytophthora Sojae ◽  
Plant Diseases ◽  
Phialophora Gregata ◽  
Diaporthe Phaseolorum ◽  
Significant Difference ◽  
Transformed Hairy Root ◽  
Soybean Genotypes

Plants recognize invading pathogens and respond biochemically to prevent invasion or inhibit colonization in plant cells. Enhancing this response in crop plants could improve sustainable methods to manage plant diseases. To enhance disease resistance in soybean, the soybean phytoalexin glyceollin was assessed in soybean hairy roots of two soybean genotypes, Spencer and PI 567374, transformed with either soybean isoflavone synthase (IFS2) or chalcone synthase (CHS6) genes that were inoculated with the soybean pathogens Diaporthe phaseolorum var. meridionales, Macrophomina phaseolina, Sclerotinia sclerotiorum, and Phytophthora sojae. The hairy-root-transformed lines had several-fold decreased levels of isoflavone daidzein, the precursor of glyceollin, and considerably lower concentrations of glyceollin induced by pathogens measured 5 days after fungal inoculation compared with the nontransformed controls without phenolic transgenes. M. phaseolina, P. sojae, and S. sclerotiorum grew much more on IFS2- and CHS6-transformed roots than on control roots, although there was no significant difference in growth of D. phaseolorum var. meridionales on the transformed hairy-root lines. In addition, glyceollin concentration was lower in D. phaseolorum var. meridionales-inoculated transformed and control roots than roots inoculated with the other pathogens. Glyceollin inhibited the growth of D. phaseolorum var. meridionales, M. phaseolina, P. sojae, S. sclerotiorum, and three additional soybean pathogens: Cercospora sojina, Phialophora gregata, and Rhizoctonia solani. The most common product of glyceollin conversion or degradation by the pathogens, with the exception of P. sojae, which had no glyceollin degradation products found in the culture medium, was 7-hydroxyglyceollin.

scholarly journals Establishment of Suspension Cell Culture from Agrobacterium-transformed Hairy Root Cells of Psammosilene tunicoides, an Endangered and Rare Medicinal Plant of China

2015 ◽  
Vol 9 (5) ◽  
pp. 379-385
Author(s):  
Zhang Zong-Shen ◽  
Luo Huai-Yu ◽  
Pan Yan ◽  
Wang Chao ◽  
Jin Zhao-Xia ◽  
...  
Keyword(s):  
Cell Culture ◽  
Medicinal Plant ◽  
Hairy Root ◽  
Suspension Cell ◽  
Suspension Cell Culture ◽  
Root Cells ◽  
Transformed Hairy Root

Production and release of pigments by culture of transformed hairy root of red beet

1992 ◽  
Vol 73 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Masahito Taya ◽  
Koji Mine ◽  
Masahiro Kino-Oka ◽  
Setsuji Tone ◽  
Takahito Ichi
Keyword(s):  
Hairy Root ◽  
Red Beet ◽  
Transformed Hairy Root

ChemInform Abstract: Sesquiterpene Lactones in Agrobacterium rhizogenes - Transformed Hairy Root Culture of Lactuca virosa

ChemInform ◽  
2010 ◽  
Vol 27 (9) ◽  
pp. no-no
Author(s):  
W. KISIEL ◽  
A. STOJAKOWSKA ◽  
J. MALARZ ◽  
S. KOHLMUENZER
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Sesquiterpene Lactones ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Lactuca Virosa ◽  
Transformed Hairy Root

Genetic transformation of Gentiana macrophylla with Agrobacterium rhizogenes: growth and production of secoiridoid glucoside gentiopicroside in transformed hairy root cultures

2006 ◽  
Vol 26 (2) ◽  
pp. 199-210 ◽  
Author(s):  
Rajesh Kumar Tiwari ◽  
Mala Trivedi ◽  
Zhang Chun Guang ◽  
Guang-Qin Guo ◽  
Guo-Chang Zheng
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Transformed Hairy Root ◽  
Gentiana Macrophylla ◽  
Secoiridoid Glucoside

Determination of lignans, phenolic acids and antioxidant capacity in transformed hairy root culture of Linum usitatissimum

2017 ◽  
Vol 32 (15) ◽  
pp. 1867-1871 ◽  
Author(s):  
Ahmed M. M. Gabr ◽  
Hoda B. Mabrok ◽  
Emam A. Abdel-Rahim ◽  
Mohamed K. El-Bahr ◽  
Iryna Smetanska
Keyword(s):  
Antioxidant Capacity ◽  
Phenolic Acids ◽  
Hairy Root ◽  
Linum Usitatissimum ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Transformed Hairy Root

scholarly journals Studies on the alkaloid production of genetically transformed and non-transformed cultures of Lobelia inflata L.

2001 ◽  
Vol 7 (2) ◽  
Author(s):  
E. Szőke ◽  
I. Bálványos ◽  
L. Kursinszki ◽  
A. Krajewska ◽  
A. Mészáros ◽  
...  
Keyword(s):  
Amino Acids ◽  
Hairy Root ◽  
Growth Regulators ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Alkaloid Production ◽  
Lobelia Inflata ◽  
Transformed Hairy Root ◽  
Total Alkaloid Content ◽  
Callus Tissues

The investigations of the growth and alkaloid production of cell suspension-, callus-, organized- and hairy root cultures from Lobelia inflata L. proved that these cultures are able to synthesize the characteristic piperidine alkaloids of the intact plant. Alkaloid precursor amino acids (Phe, Lys) and plant growth regulators affect not only the growth and differentiation of tissue cultures but also their secondary metabolism. The synthetic regulator Sz/I I combined with Phe increased the total alkaloid content considerably in callus- and organized cultures; regulator Sz/28 especially increased the lobeline content (in organized cultures in response to Lys, in callus tissues as a result of Phe application). With the aim of optimizing growth and alkaloid production of the genetically transformed hairy root cultures of Lobelia inflata L. we studied the effect of some growth regulators (NAA, IAA, kinetin) and precursor amino acids (Lys, Phe). The kinetin had inhibiting effect on the growth and lobeline production of the hairy roots. The IAA and NAA increased the biomass formation and lobeline production. The highest lobeline level was detected in tissues cultivated on hormone-free medium containing Phe.  

scholarly journals Efficient genetic transformation of Impatiens hawkerii Bull. (Balsamiaceae) using agrobacterium rhizogenes

2009 ◽  
Vol 61 (3) ◽  
pp. 467-474 ◽  
Author(s):  
Snezana Milosevic ◽  
Angelina Subotic ◽  
A. Cingel ◽  
Sladjana Jevremovic ◽  
Slavica Ninkovic
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Lateral Roots ◽  
Basal Medium ◽  
Growth Patterns ◽  
Pcr Analysis ◽  
Gus Assay ◽  
Hairy Root Clones ◽  
First Time

Transformation of Impatiens hawkerii Bull. mediated by Agrobacterium rhizogenes strain A4M70GUS was studied. Hairy roots developed 10 days after inoculation were excised from the shoot explants and transferred onto Murashige and Skoog's (MS) basal medium lacking plant growth regulators. More than 20 hairy root clones were established and eight of them were further analyzed. Each clone differed significantly from the others in growth capacity and lateral branching. Clone C2 showed the highest biomass (20.6 g L-1) as well as the highest number of lateral roots (37 ? 2.2). The transgenic nature of the established hairy root clones was confirmed by GUS assay and PCR analysis. In conclusion, hairy roots were developed for the first time in I. hawkerii Bull., and transgenic hairy root clones showed a distinct morphological nature and growth patterns.

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