scholarly journals Xanthones Production in Gentiana dinarica Beck Hairy Root Cultures Grown in Simple Bioreactors

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1610
Author(s):  
Branka Vinterhalter ◽  
Nevena Banjac ◽  
Dragan Vinterhalter ◽  
Dijana Krstić-Milošević
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Bubble Column ◽  
Growth Parameters ◽  
Growth Index ◽  
Dry Weight ◽  
Growth Conditions ◽  
Good Growth ◽  
Gentiana Dinarica ◽  
Hairy Root Clones

The hairy root clones of Gentiana dinarica cl-B, cl-D, cl-3, and cl-14 were cultivated in parallel in diverse simple bioreactors, including temporary immersion systems RITA® (TIS RITA®), bubble column bioreactors (BCB), and Erlenmeyer flasks (EF), and evaluated for biomass production and xanthone content. The obtained results showed that TIS RITA® and BCB containing ½ MS medium with 4% sucrose provided equally good growth conditions in which the majority of the clones displayed the higher percentage of dry matter (DM%), and xanthones norswertianin-1-O-primeveroside (nor-1-O-prim) and norswertianin production than those cultivated in EF. Thin and well branched hairy root clone cl-B grown in BCB for 7 weeks was superior regarding all growth parameters tested, including growth index (19.97), dry weight (2.88 g), and DM% (25.70%) compared to all other clones. Cl-B cultured in TIS RITA® contained the highest amount of nor-1-O-prim (56.82 mg per vessel). In BCB with constant aeration, cl-B accumulated the highest norswertianin content reaching 18.08 mg/vessel. The optimized conditions for cultivation of selected G. dinarica hairy root clones in highly aerated TIS RITA® and BCB systems contribute to the development of bioreactor technology designed for the large scale commercial production of xanthones nor-1-O-prim and norswertianin.

scholarly journals Effect of salicylic acid on pre-transplant acclimatization of native tomato populations

2018 ◽  
Vol 36 (4) ◽  
pp. 480-485
Author(s):  
Wilberth A Poot-Poot ◽  
Rafael Delgado-Martínez ◽  
Sergio Castro-Nava ◽  
Ma Teresa Segura-Martínez ◽  
Alejandro Carreón-Pérez ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Growth And Development ◽  
Growth Parameters ◽  
Field Capacity ◽  
Dry Weight ◽  
Growth Conditions ◽  
Peat Moss ◽  
Hypocotyl Length ◽  
Cell Processes ◽  
Recorded Data

ABSTRACT The aim of this study was to evaluate the effect of salicylic acid (SA) application on pre-transplant acclimatization of native populations of tomato. SA is a growth regulator that modifies plant growth and development by inducing changes in cell processes, physiology and morphology. Five populations of native tomato were sown in polystyrene trays. Peat moss was used as substrate and plants were maintained at field capacity continuously. After emergence, seedlings were applied during three weeks with different concentration of SA (0.0, 0.01, 0.1, 0.5 and 1.0 μM). A completely random experimental design was used with five replications per treatment. The growth parameters evaluated were height, stem diameter, number of leaves, hypocotyl length, shoot fresh and dry weight (leaves and stem), and root length and fresh and dry weight. An analysis of variance was carried out, and means were compared with the Tukey test (5%) using SAS statistical software. The recorded data show that pre-transplant seedlings of each of the evaluated populations responded significantly (P=0.01) to the SA treatments. Also, the comparison of means of each of the factors under study showed positive changes. With the concentrations of 0.5 and 0.1 μM SA, higher values of the studied variables were obtained than with the concentrations 0.01 and 1.0 μM SA. The native tomato populations sprayed with SA at concentrations of 0.5 and 0.1 μM responded positively in terms of seedling growth and development. Based on these findings, SA treatments can help acclimatize and present better growth conditions to the seedlings before being transplanted.

Incorporation de résidus organiques dans un substrat tourbeux pour la production d'impatiens et de géraniums

1998 ◽  
Vol 78 (1) ◽  
pp. 131-138
Author(s):  
Fabienne Gauthier ◽  
Serge Gagnon ◽  
Blanche Dansereau
Keyword(s):  
Key Words ◽  
Growth Parameters ◽  
Growth Index ◽  
Visual Quality ◽  
Dry Weight ◽  
Peat Moss ◽  
Paper Sludge ◽  
Organic Residues ◽  
Top Dry Weight ◽  
Impatiens Wallerana

During winter 1994 and spring-summer 1994, seedlings of Impatiens wallerana 'Accent Coral' and Pelargonium × hortorum 'Orbit Hot Pink' were grown in a commercial substrate (PRO-MIX 'BX') or in one of 24 substrates composed of perlite (35% by volume), peat moss and of six organic residues (composted water-treated sludge, forestry compost, fresh or composted used peat extracted by biofilter during treatment of municipal water, and fresh or composted paper sludge). The purpose of the study was to determine the maximal proportion of these residues to be incorporated into a peat substrate to obtain quality plants. Growth parameters (growth index, top dry weight, and visual quality) generally decreased with increasing proportion (5, 10, 25 or 40% per volume). During the winter experiment, growth of control plants was superior to growth of plants grown in substrates composed of residues. An incorporated proportion of 25% per volume was shown to be maximal for the production of impatiens and geraniums. Key words: Impatiens wallerana, Pelargonium × hortorum, water treated sludge, composted wastes, peat moss, subirrigation

scholarly journals Variations in Morphological Characters and Antioxidant Potential of Different Plant Parts of Four Ziziphus Mill. Species from the Cholistan

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2734
Author(s):  
Muhammad Umair Riaz ◽  
Muhammad Ali Raza ◽  
Amjad Saeed ◽  
Mukhtar Ahmed ◽  
Tanveer Hussain
Keyword(s):  
Large Scale ◽  
Positive Impact ◽  
Dry Weight ◽  
Growth Conditions ◽  
Morphological Characters ◽  
Leaf Length ◽  
Important Species ◽  
Medicinal Uses ◽  
Leaf Petiole ◽  
Plant Parts

Genus Ziziphus (Z.) contains various important species in tropical and subtropical regions that are globally famous for their food and medicinal uses. However, no comprehensive study was available on the morphology and phytochemistry of Ziziphus species, mainly under different growth conditions, i.e., irrigated and desert (Cholistan). Therefore, this study was carried out to evaluate the morphological and phytochemical characteristics of Ziziphus species, i.e., Z. jujuba, Z. mauritiana, Z. spina-christi, and Z. nummularia, found in the irrigated and desert conditions. Our results revealed significant variations for most of the measured parameters, showing a large-scale diversity among Ziziphus species under irrigated and desert conditions. Specifically, Ziziphus species showed better morphology of all measured parameters of leaves and fruits under irrigated conditions compared to desert conditions, indicating that the optimum water availability in irrigated conditions improved the morphological parameters of Z. species. Meanwhile, among all Ziziphus species, the maximum leaf length (7.4 cm), leaf width (4.1 cm), leaf area (30.6 cm2), and leaf petiole length (1.3 cm) were observed for Z. jujuba, and the highest leaf dry weight (55.4%) was recorded for Z. mauritiana. Similarly, the highest fruit length (3.9 cm), fruit stalk length (1.5 cm), fruit diameter (3.6 cm), fruit width (3.8 cm), fruit area (66.1 cm2), seed length (2 cm), and seed diameter (1.1 cm) were measured for species Z. jujuba, while the maximum fruit dry weight (49.9%) and seed width (1.4 cm) were recorded for species Z. nummularia. Interestingly, compared to irrigated conditions, higher values of bioactive contents, i.e., phenol, flavonoid, and antioxidant activity, in fruits and leaves of Ziziphus species under desert conditions indicated the positive impact of desert climate on the phytochemistry of the Z. plants. Among Ziziphus species, Z. nummularia accumulated the maximum fruit phenols (304.4 mg GAE/100 g), leaf phenols (314.2 mg GAE/100 g), fruit flavonoids (123.7 mg QE/100 g), and leaf flavonoids (113.4 mg QE/100 g). Overall, this study demonstrated the significant morphological and phytochemical variations of the Ziziphus species under irrigated and desert conditions, which could be utilized for future studies to improve the production and medicinal potential of the Ziziphus, especially in desert areas.

scholarly journals Optimization of cultivation conditions of Salvia viridis L. shoots in the Plantform bioreactor to increase polyphenol production

2021 ◽  
Author(s):  
Izabela Grzegorczyk-Karolak ◽  
Paulina Staniewska ◽  
Liwia Lebelt ◽  
Dorota G. Piotrowska
Keyword(s):  
Phenolic Acid ◽  
Large Scale ◽  
Growth Parameters ◽  
Shoot Culture ◽  
Continuous System ◽  
Dry Weight ◽  
Total Phenolic ◽  
Efficient Production ◽  
Anticancer Activities ◽  
Batch System

AbstractSalvia viridis is a rich source of pharmacologically-important polyphenolic compounds with anti-inflammatory, antioxidant and anticancer activities. The aim of the present study was to optimise the large-scale cultivation of sage shoots in the Plantform bioreactor. It evaluated the effect of culture duration (two or three weeks), the use of a semi-continuous system and the immersion frequency in a liquid medium (every 80 or 170 min) on the growth and accumulation of secondary metabolites. The content of bioactive phenolic acids and phenylethanoids in the obtained material was determined using HPLC. The most efficient growth parameters, expressed as fresh (26.99 g) and dry weight (3.51 g) as well as proliferation ratio (18.6), were observed for shoots grown in a fed-batch system, immersed every 80 min; this approach yielded a 33-fold biomass increase within four weeks. However, the most efficient production of bioactive compounds was achieved for shoots grown for three weeks in a batch system, immersed every 80 min: total phenolic acid, total phenylethanoid and total phenol contents of 18.3, 11.4 and 29.7 mg/g DW. In these conditions, within three weeks, total phenolic acid level was almost 10 times higher than that found in the aerial parts of four-month-old soil-grown plants with a similar phenylethanoid level. Hence, the described S. viridis shoot culture cultivated in the Plantform bioreactor may be an alternative efficient source of plant material rich in valuable compounds.

scholarly journals Positive effects of duckweed polycultures on starch and protein accumulation

2016 ◽  
Vol 36 (5) ◽  
Author(s):  
Yang Li ◽  
Fantao Zhang ◽  
Maurycy Daroch ◽  
Jie Tang
Keyword(s):  
Protein Content ◽  
Large Scale ◽  
Growth Parameters ◽  
Biomass Accumulation ◽  
Growth Conditions ◽  
Protein Accumulation ◽  
Nitrogen And Phosphorus ◽  
Spirodela Polyrhiza ◽  
Relative Growth ◽  
Positive Effects

The effect of duckweed species composition (Lemna aequinoctialis 5505, Landoltia punctata 5506 and Spirodela polyrhiza 5507) in polyculture and monoculture on biomass and starch/protein content were investigated at different levels of temperature, light intensity, nitrogen and phosphorus concentrations. The three growth parameters significantly affect duckweed biomass accumulation. Different combinations of duckweed species greatly varied in starch/protein content. Although all the polycultures showed a median relative growth rate and the majority of the polycultures showed a median and starch/protein content as compared with their respective monocultures, some of the polycultures were found to promote the accumulation of starch/protein at different growth conditions. These findings indicated that proper combination of duckweed species could facilitate desirable biomass accumulation and improve biomass quality. The present study provides useful references for future large-scale duckweed cultivation.

scholarly journals Growth of Basil (Ocimum basilicum) in Aeroponics, DRF, and Raft Systems with Effluents of African Catfish (Clarias gariepinus) in Decoupled Aquaponics (s.s.)

2021 ◽  
Vol 3 (3) ◽  
pp. 559-574
Author(s):  
Johannes Pasch ◽  
Samuel Appelbaum ◽  
Harry Wilhelm Palm ◽  
Ulrich Knaus
Keyword(s):  
Large Scale ◽  
Growth Parameters ◽  
Clarias Gariepinus ◽  
Dry Weight ◽  
Ocimum Basilicum ◽  
African Catfish ◽  
Floating Raft ◽  
Tropical Conditions ◽  
Intensive Rearing ◽  
System Improvement

Basil (Ocimum basilicum) was cultivated in three hydroponic subsystems (i) a modified commercial aeroponics, (ii) a dynamic root floating (DRF) system, and (iii) a floating raft system in a decoupled aquaponic system in Northern Germany, Mecklenburg–Western Pomerania. For plant nutrition, aquaculture process water from intensive rearing of African catfish (Clarias gariepinus) was used without fertilizer. After 39 days, 16 plant growth parameters were compared, with aeroponics performing significantly better in 11 parameters compared with the DRF, and better compared with the raft in 13 parameters. The economically important leaf wet and dry weight was over 40% higher in aeroponics (28.53 ± 8.74 g; 4.26 ± 1.23 g), but similar in the DRF (20.19 ± 6.57 g; 2.83 ± 0.90 g) and raft (20.35 ± 7.14 g; 2.84 ± 1.04 g). The roots in the DRF grew shorter and thicker; however, this resulted in a higher root dry weight in aeroponics (1.08 ± 0.38 g) compared with the DRF (0.82 ± 0.36 g) and raft (0.67 ± 0.27 g). With optimal fertilizer and system improvement, aquaponic aeroponics (s.s.) could become a productive and sustainable large-scale food production system in the future. Due to its simple construction, the raft is ideal for domestic or semi-commercial use and can be used in areas where water is neither scarce nor expensive. The DRF system is particularly suitable for basil cultivation under hot tropical conditions.

scholarly journals Variability of Bioactive Glucosinolates, Isothiocyanates and Enzyme Patterns in Horseradish Hairy Root Cultures Initiated from Different Organs

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2828 ◽  
Author(s):  
Bertóti ◽  
Böszörményi ◽  
Alberti ◽  
Béni ◽  
M-Hamvas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Horseradish Peroxidase ◽  
Hairy Root ◽  
Growth Index ◽  
Biologically Active ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Daily Growth ◽  
Hairy Root Clones ◽  
Enzyme Patterns

Horseradish hairy root cultures are suitable plant tissue organs to study the glucosinolate–myrosinase–isothiocyanate system and also to produce the biologically active isothiocyanates and horseradish peroxidase, widely used in molecular biology. Fifty hairy root clones were isolated after Agrobacterium rhizogenes infection of surface sterilized Armoracia rusticana petioles and leaf blades, from which 21 were viable after antibiotic treatment. Biomass properties (e.g. dry weight %, daily growth index), glucosinolate content (analyzed by liquid chromatography-electronspray ionization-mass spectrometry (LC-ESI-MS/MS)), isothiocyanate and nitrile content (analyzed by gas chromatography-mass spectrometry (GC-MS)), myrosinase (on-gel detection) and horseradish peroxidase enzyme patterns (on-gel detection and spectrophotometry), and morphological features were examined with multi-variable statistical analysis. In addition to the several positive and negative correlations, the most outstanding phenomenon was many parameters of the hairy root clones showed dependence on the organ of origin. Among others, the daily growth index, sinigrin, glucobrassicin, 3-phenylpropionitrile, indole-3-acetonitrile and horseradish peroxidase values showed significantly higher levels in horseradish hairy root cultures initiated from leaf blades.

scholarly journals Effect of container and potting media on raising quality seedlings of Acacia auriculiformis in the nursery

2019 ◽  
Vol 3 (01) ◽  
Author(s):  
MD. ARIFUL ISLAM ◽  
RAYHANUR RAHMAN ◽  
MOHAMMED KAMAL HOSSAIN
Keyword(s):  
Root Length ◽  
Quality Index ◽  
Large Scale ◽  
Growth Parameters ◽  
Block Design ◽  
Dry Weight ◽  
Acacia Auriculiformis ◽  
Cow Dung ◽  
Randomized Block Design ◽  
Potting Media

Abstract. Islam MDA, Rahman MDA, Hossain MK. 2019. Effect of container and potting media on raising quality seedlings of Acacia auriculiformis in the nursery. Asian J Agric 3: 26-32. The study elucidates the effect of container and potting media on raising quality seedlings of Acacia auriculiformis in the nursery of the Institute of Forestry and Environmental Sciences, University of Chittagong to find out a suitable container and potting media for raising large scale quality seedlings. The seedlings were evaluated by five container and seven potting media treatments for eight months. A Completely Randomized Block Design (CRBD) was adopted for the study with three replications for each treatment. The Analysis of variance (ANOVA) and Duncan’s Multiple Range Test (DMRT) was tested for the analysis to explore the possible treatment variations. However, the study reveals nodulation with growth parameters; shoot and root dry biomass production and quality index were highest in 20 cm × 15 cm size polybags whereas the highest root length and shoot-root ratio was observed in 15 cm × 10 cm and 15 cm × 13 cm size polybag respectively. Considering the potting media, highest nodulation, growth parameters, shoot dry and fresh weight, shoot- root ratio and biomass were found in combination of soil + cow dung + phosphorus (0.16 g/polybag). Highest root length, root fresh and dry weight, and quality index were found in the combination of soil + cow dung (3:1). Therefore, it is recommended that containers of 20 cm × 15 cm size polybag and with a potting media of soil + cow dung + phosphorus (3 parts soil, 1 parts cow dung + 0.16 g/polybag) combination produce quality A. auriculiformis seedlings in the nursery.

Effect of rhizobacteria strains on the induction of resistance in barley genotypes against Cochliobolus sativus

2020 ◽  
Vol 13 (2) ◽  
pp. 83-92 ◽  
Author(s):  
A. Adam
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Growth Parameters ◽  
Disease Incidence ◽  
Dry Weight ◽  
Cochliobolus Sativus ◽  
Resistance Level ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Barley Genotypes

SummaryEnhancement of the resistance level in plants by rhizobacteria has been proven in several pathosystems. This study investigated the ability of four rhizobacteria strains (Pseudomonas putida BTP1 and Bacillus subtilis Bs2500, Bs2504 and Bs2508) to promote the growth in three barley genotypes and protect them against Cochliobolus sativus. Our results demonstrated that all tested rhizobacteria strains had a protective effect on barley genotypes Arabi Abiad, Banteng and WI2291. However, P. putida BTP1 and B. subtilis Bs2508 strains were the most effective as they reduced disease incidence by 53 and 38% (mean effect), respectively. On the other hand, there were significant differences among the rhizobacteria-treated genotypes on plant growth parameters, such as wet weight, dry weight, plant height and number of leaves. Pseudomonas putida BTP1 strain was the most effective as it significantly increased plant growth by 15-32%. In addition, the susceptible genotypes Arabi Abiad and WI2291 were the most responsive to rhizobacteria. This means that these genotypes have a high potential for increase of their resistance against the pathogen and enhancement of plant growth after the application of rhizobacteria. Consequently, barley seed treatment with the tested rhizobacteria could be considered as an effective biocontrol method against C. sativus.

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