Molecular identification and phylogenetic status of marineBacillus associated with coral sediment, showing antibacterial effects against human pathogens

2008 ◽  
Vol 58 (2) ◽  
pp. 309-312 ◽  
Author(s):  
Shruti Chatterjee ◽  
Soumya Haldar ◽  
Masahiro Asakura ◽  
Shinji Yamasaki ◽  
Thangavel Balasubramanian
Keyword(s):  
Molecular Identification ◽  
Human Pathogens ◽  
Antibacterial Effects ◽  
Phylogenetic Status

scholarly journals Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Nursenem Karaca ◽  
Görkem Şener ◽  
Betül Demirci ◽  
Fatih Demirci
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Antibacterial Effect ◽  
Synergistic Effects ◽  
Pharmaceutical Formulations ◽  
Human Pathogens ◽  
Broth Microdilution ◽  
Antibacterial Effects ◽  
Lavandula Latifolia

AbstractCombination of various compounds and essential oils for pharmaceutical formulations withdraw attention. In this present study, it was aimed to evaluate the in vitro potential synergistic antibacterial effect of Lavandula latifolia (spike lavender) essential oil with camphor by using the checkerboard method against the human pathogens; Staphylococcus aureus and Listeria monocytogenes. Pharmacopoeia quality L. latifolia essential oil and racemic camphor were analyzed and verified by GC-FID and GC/MS, simultaneously. In vitro antibacterial activity of essential oil and camphor (MIC range: 0.16–20 mg/mL) and standard antimicrobial clarithromycin (MIC range: 0.125–16 μg/mL) were carried out by broth microdilution against S. aureus and L. monocytogenes standard strains, respectively. Resulting antibacterial effects were evaluated for their fractional inhibitory concentrations (FICs) as antagonistic, additive and synergistic effects. The analytical results showed that the major component of essential oil was linalool (45.2%) and 1,8-cineole (25.6%). Antibacterial effects of essential oil were determined as MIC 1.25–5 mg/mL. As a result of the experiments, L. latifolia essential oil–camphor combinations were identified as “synergistic (FIC ≤ 0.5), and additive (0.5 < FIC ≤ 1)” in the respective combinations, suggesting further evaluation for formulations for potential antimicrobial applications in food and pharmaceuticals.

scholarly journals Antibacterial effects of Lactobacillus isolates of curd and human milk origin against food-borne and human pathogens

3 Biotech ◽  
2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Chetan Sharma ◽  
Brij Pal Singh ◽  
Nishchal Thakur ◽  
Sachin Gulati ◽  
Sanjolly Gupta ◽  
...  
Keyword(s):  
Human Milk ◽  
Human Pathogens ◽  
Antibacterial Effects ◽  
Food Borne

Morphological and molecular identification of a lung fluke, Paragonimus macrorchis (Trematoda, Paragonimidae), found in central Lao PDR and its molecular phylogenetic status in the genus Paragonimus

2015 ◽  
Vol 64 (6) ◽  
pp. 513-518 ◽  
Author(s):  
Oranuch Sanpool ◽  
Pewpan Maleewong Intapan ◽  
Tongjit Thanchomnang ◽  
Penchom Janwan ◽  
Sakhone Laymanivong ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Lao Pdr ◽  
Phylogenetic Status ◽  
Molecular Phylogenetic ◽  
Lung Fluke

scholarly journals Molecular Identification, Phylogenetic Status, and Geographic Distribution of Culicoides oxystoma (Diptera: Ceratopogonidae) in Israel

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33610 ◽  
Author(s):  
Neta Morag ◽  
Yonatan Saroya ◽  
Yehuda Braverman ◽  
Eyal Klement ◽  
Yuval Gottlieb
Keyword(s):  
Geographic Distribution ◽  
Molecular Identification ◽  
Culicoides Oxystoma ◽  
Phylogenetic Status

Antibacterial Effects of Aloe Barbedensis Miller (Aloe Vera) Leaf Extract on Some Common Human Pathogens

2018 ◽  
Vol 7 (1) ◽  
Author(s):  
Ezenwa C. M ◽  
Emukah E. ◽  
Nnagbo P. A ◽  
Obasi C. C. ◽  
Ohabuiro B. N. C ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Aloe Vera ◽  
Human Pathogens ◽  
Antibacterial Effects

scholarly journals Isolation, Molecular identification, Molecular docking In Vitro and In Silico Evaluation study against Fusarium oxysporum sp. Lactucae

2021 ◽  
Author(s):  
Israa M Shamkh ◽  
Dina Pratiwi ◽  
Ahmed S El faki
Keyword(s):  
Molecular Docking ◽  
Fusarium Oxysporum ◽  
Molecular Identification ◽  
Hydroxypropyl Methylcellulose ◽  
Pathological Process ◽  
Infection Process ◽  
Large Species ◽  
Human Pathogens ◽  
Molecular Docking Study

Fusarium oxysporum is a large species complex of both plant and human pathogens that attack a diverse array of species in a host-specific manner. Fusarium is a plant disease that lives in the soil. It penetrates the plant that leads to the loss of productivity of crops. The flexibility of casing the pathological process of the several Fusarium virulence pathways. The aims of this study were the fungus strain isolation and molecular identification of F. oxysporum f. sp. Lactucae, in addition to the Insilco study using molecular docking analysis to determine enzyme and plant phytochemicals from neem and black cumin responsible for inhibition of the pathological process of the Fusarium virulence pathways and prevent the initiation of the infection process. The causal pathogen of lettuce wilt was isolated, tested its ability to infect the lettuce plant under artificial infection, molecularly identified it and theoretically determined the most active natural compounds against it by molecular docking technique. Our results revealed that the typical symptoms of lettuce Fusarium wilt were shown when the plants are grown in soil inoculated with the pathogen. The pathogen is identified as F. oxysporum f. sp. lactucae race 5 and the sequence of this F. oxysporum was given the accession number MT249304.1. Moreover, it is known that the Azadirachta indica and Nigella stevia from previous studies have a fungicide effect, but the cause was not known in this study for the first time the substances responsible for this effect were identified. On the other hand, a homology modeling and molecular docking study were carried out on the most active compounds against F oxysporum .a fungus, in order to understand and determine the molecular interactions taking place between the ligand and the corresponding receptor of the studied target. Results indicated that active ingredients were Azadirachtin2D, Longicyclene 2D, Stearic acid 2D, Neohesperidin, Hydroxypropyl methylcellulose, and Vilasinin 2 D exhibited the best docking scores and interaction profiles within the active site of Fusarium spp. enzymes. After data analyses, information on mycelial growth inhibitors, structural requirements, and putative enzyme targets may be used in further antifungal development based on phytoalexin analogs for controlling phytopathogens.

Electron microscopy in diagnostic virology

1984 ◽  
Vol 42 ◽  
pp. 70-73
Author(s):  
S. E. Miller
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Molecular Identification ◽  
Point Of View ◽  
Negative Stain ◽  
Viral Diagnosis ◽  
Electron Microscopist ◽  
Diagnostic Virology

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.

Antibacterial Effects of Lactoferricin B

1999 ◽  
Vol 31 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Lars H. Vorland, Hilde Ulvatne, Jill And
Keyword(s):  
Antibacterial Effects
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