A titration calorimetry study of a technical grade APG

Exposure of young pepper plants to chilling temperatures delays the development of terminal flower buds to flowering during post-stress growth. Degree of adverse influence depends on chilling intensity, exposure duration and varietal sensitivity. `Ma Belle' pepper plants were grown in a greenhouse (GH) during winter months on the St. Paul campus, No supplemental lighting was provided. When plants were at the 2- to 3-leaf stage, they were foliar sprayed with mefluidide (Technical grade) at 0, 5, 10 and 15 ppm. One day after treatment, some plants were transferred from GH to a cold room (3° ∼4°C day/night) with 12-h photoperiod. Treatad plants remaining in the GH served as the control. Plants were chilled for 1, 2, 4 and 6 days and then brought back to the GH for post-stress growth and development observation. Treated and untreated plants grown in the GH showed no difference in days to flowering, and reached 50% flowering at about 62 days after treatment. When untreated plants were chilled for 1,2,4 and 6 days, they showed a delay of 8, 18, 30 and 34 days, respectively, to flowering, If not killed, as compared to the control The long delay to flowering was due to the injury of the terminal flower buds. After 4 and 6 days of chilling, most terminal flower buds were killed. However, when plants were treated with mefluidide and subsequently chilled days to flowering were significantly shortened. A difference of 10-12 days was observed between chilled untreated plants and chilled treated plants. Concentrations of 5 to 15 ppm were equally effective in protection against chilling.

Influence of Ascorbic Acid on the Structure and Function of Alpha-2- macroglobulin: Investigations using Spectroscopic and Thermodynamic Techniques

Protein and Peptide Letters ◽

10.2174/0929866526666191002113525 ◽

2020 ◽

Vol 27 (3) ◽

pp. 201-209

Author(s):

Syed Saqib Ali ◽

Mohammad Khalid Zia ◽

Tooba Siddiqui ◽

Haseeb Ahsan ◽

Fahim Halim Khan

Keyword(s):

Ascorbic Acid ◽

Conformational Changes ◽

Structural Changes ◽

The Body ◽

Titration Calorimetry ◽

Spectroscopic Techniques ◽

Human Beings ◽

Plasma Ascorbic Acid ◽

Dietary Antioxidant ◽

And Function

Background: Ascorbic acid is a classic dietary antioxidant which plays an important role in the body of human beings. It is commonly found in various foods as well as taken as dietary supplement. Objective: The plasma ascorbic acid concentration may range from low, as in chronic or acute oxidative stress to high if delivered intravenously during cancer treatment. Sheep alpha-2- macroglobulin (α2M), a human α2M homologue is a large tetrameric glycoprotein of 630 kDa with antiproteinase activity, found in sheep’s blood. Methods: In the present study, the interaction of ascorbic acid with alpha-2-macroglobulin was explored in the presence of visible light by utilizing various spectroscopic techniques and isothermal titration calorimetry (ITC). Results: UV-vis and fluorescence spectroscopy suggests the formation of a complex between ascorbic acid and α2M apparent by increased absorbance and decreased fluorescence. Secondary structural changes in the α2M were investigated by CD and FT-IR spectroscopy. Our findings suggest the induction of subtle conformational changes in α2M induced by ascorbic acid. Thermodynamics signatures of ascorbic acid and α2M interaction indicate that the binding is an enthalpy-driven process. Conclusion: It is possible that ascorbic acid binds and compromises antiproteinase activity of α2M by inducing changes in the secondary structure of the protein.

Separation and Determination of Some Aromatic Sulfones by Reversed-Phase High-Performance Liquid Chromatography

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19940569 ◽

1994 ◽

Vol 59 (3) ◽

pp. 569-574 ◽

Cited By ~ 1

Author(s):

Josef Královský ◽

Marta Kalhousová ◽

Petr Šlosar

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Mobile Phase ◽

High Performance ◽

Reversed Phase ◽

Uv Spectra ◽

Optimum Conditions ◽

Linear Calibration ◽

Technical Grade

The reversed-phase high-performance liquid chromatography of some selected, industrially important aromatic sulfones has been investigated. The chromatographic behaviour of three groups of aromatic sulfones has been studied. The optimum conditions of separation and UV spectra of the sulfones and some of their hydroxy and benzyloxy derivatives are presented. The dependences of capacity factors vs methanol content in mobile phase are mentioned. The results obtained have been applied to the quantitative analysis of different technical-grade samples and isomer mixtures. For all the separation methods mentioned the concentration ranges of linear calibration curves have been determined.

Polyelectrolyte complex characterization with isothermal titration calorimetry and colloid titration

Colloids and Surfaces A Physicochemical and Engineering Aspects ◽

10.1016/j.colsurfa.2007.11.053 ◽

2008 ◽

Vol 317 (1-3) ◽

pp. 535-542 ◽

Cited By ~ 47

Author(s):

Xianhua Feng ◽

Marc Leduc ◽

Robert Pelton

Keyword(s):

Isothermal Titration Calorimetry ◽

Polyelectrolyte Complex ◽

Titration Calorimetry ◽

Colloid Titration ◽

Complex Characterization

Preparation of alcohol-based handrub in COVID-19 Alsatian cluster

Pharmaceutical Technology in Hospital Pharmacy ◽

10.1515/pthp-2020-0004 ◽

2020 ◽

Vol 5 (1) ◽

Author(s):

Elodie Di-Falco ◽

Johan Bourbon ◽

Isalyne Sbaffe ◽

Jean-Daniel Kaiser

Keyword(s):

Critical Period ◽

Hospital Management ◽

Management Team ◽

The Other ◽

Packaging Materials ◽

Local Production ◽

Technical Grade ◽

Hand Sanitizer ◽

Set Up ◽

Real Challenge

AbstractAlsace, in particular Haut-Rhin, is one of the main clusters of COVID-19 in France. There has been a shortage of essential supplies in the area, especially alcohol-based hand sanitizer. In this context, and in accordance with the decree dated March 6, 2020, our hospital management team asked us to start local production of alcohol-based handrub. This was a real challenge: In one week, we had to implement the production of handrub to meet the needs of a 1,400-bed hospital. The production had to comply with the French preparation guidelines and take place on specific premises, with qualified and calibrated equipment, by qualified staff, under the supervision of a pharmacist. The other big challenge we faced was the supply of pharmaceutical raw and packaging materials. During this particular critical period, all suppliers were out of stock. Here, we describe the organizational set-up and the decisions made, e. g., to use technical-grade ethanol before the publication of the decrees dated March 13 and March 23, 2020.