Bioassay-guided isolation and identification of anti-Alzheimer’s active compounds from Spiranthes sinensis (Pers.) Ames

2021 ◽  
Author(s):  
Meijia Zou ◽  
Runkun Wang ◽  
Qimeng Yin ◽  
Liang Liu
Keyword(s):  
Isolation And Identification ◽  
Active Compounds ◽  
Spiranthes Sinensis

Bioassay-Guided Isolation and Identification of a Acetylcholinesterase Inhibitors from Calophyllum polyanthum

2013 ◽  
Vol 634-638 ◽  
pp. 1225-1228 ◽  
Author(s):  
Fei Zhang ◽  
Hai Wei Ren ◽  
Yong Gang Wang ◽  
Rong Wang ◽  
Jie Sheng
Keyword(s):  
Inhibitory Activity ◽  
Cholinesterase Activity ◽  
Spectral Characteristics ◽  
Ethanolic Extract ◽  
Acetylcholinesterase Inhibitors ◽  
Microplate Assay ◽  
Acetyl Cholinesterase ◽  
Isolation And Identification ◽  
Active Compounds ◽  
Bioautographic Method

A significant acetylcholinesterase inhibitory activity was observed for the Ethanolic extract from the leaves of Calophyllum polyanthum by using TLC bioautographic method. Further bioassay-guided isolation of this extract using TLC bioautographic method resulted in obtaining a pyranochromanone, apetalic acid (1). The structure of 1 was identified by comparison of it’s spectral characteristics with previous reports. The concentration required for 50% inhibition of 1 was 0.95 mM, determined by a microplate assay. The anti-acetylcholinesterase aity of compound 1 was weak, but it was the first pyranochromanone which have anti-acetyl cholinesterase activity. As a new leading compound, it can be modified and transformed to obtain more potently active compounds.

Isolation and identification of active compounds from Drimys winteri barks

1998 ◽  
Vol 62 (3) ◽  
pp. 223-227 ◽  
Author(s):  
Valdir Cechinel Filho ◽  
Valfredo Schlemper ◽  
Adair R.S Santos ◽  
Tânia R Pinheiro ◽  
Rosendo A Yunes ◽  
...  
Keyword(s):  
Isolation And Identification ◽  
Active Compounds ◽  
Drimys Winteri

scholarly journals Ямаан арц (Juniperus sibirica Burgsd.)-ны мутагений үйлчлэлийг дарангуйлах идэвхийн судалгаа

2017 ◽  
pp. 14-24
Author(s):  
Одонтуяа Г ◽  
Батхүү Ж
Keyword(s):  
Salmonella Typhimurium ◽  
Plant Extracts ◽  
Life Quality ◽  
Antimutagenic Activity ◽  
Isolation And Identification ◽  
Traditional Use ◽  
Cytotoxic Effects ◽  
Active Compounds ◽  
Aerial Parts ◽  
Mongolian Flora

This study evaluated the antimutagenic and cytotoxic effects of some plants growing in Mongolia and plants were selected on the basis of their traditional use in various diseases. Over the past years numbers of cancer incidences are increasing in Mongolia. There are accounted several reasons, in particular the use of less guaranteed imported food, heavy air pollution, environmental contamination and low life quality of the population. Therefore, we attempted to search antimutagenic activity among plants from the Mongolian flora and possibility of their use for preventing and treating cancer cases. The study might promote the way to fight with cancer, if we can find antimutagenic active compounds from natural crude drugs, including medicinal plants.We have examined the antimutagenic and cytotoxic effects of 20 samples, which prepared from different parts of 10 plants species. The antimutagenic and cytotoxic tests were performed as described by Ames. The bacteria used in the test are a strain of Salmonella typhimurium TA1537, and using 9-aminoacridine as direct mutagen.From the 20 plant extracts studied, extracts of Achnatherum splendens Trin. (aerial parts), Cynoglossum divaricatum Steph. (roots, stems), Juniperus sibirica Burgsd. (leaf, stem) strongly inhibited mutagenicity of 9-aminoacridine and their inhibition activity was 49.7%, 41.5%, 61.5%, 40% and 43% respectively. All plant extracts have not any cytotoxic effect on Salmonella typhimurium TA1537.From the high active plants Juniperus sibirica Burgsd. (aerial parts) which was selected for further detailed study 3 pure active compounds as JS-1, JS-2 and JS-3 were isolated. The compound JS-3 showed the high (55%) antimutagenic activity and its molecular structure was determined as cetyl alcohol or hexadecanol-1 according to its physical and chemical characteristics as well as 1H-NMR and 13C-NMR spectroscopy analysis.We have revealed that the availability of plants possessing antimutagenic activity among the Mongolian flora. Consequently, it is needed further detailed study of active plants, isolation and identification of active pure compounds, as well as possible application of the active plant products in the medicinal practice.

scholarly journals Study the Inhibitory effect of Rose and blossom bitter orange flowers extracts against different types of bacteria isolated from the oral cavity

2014 ◽  
Vol 11 (2) ◽  
pp. 795-802
Author(s):  
Baghdad Science Journal
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Cold Water ◽  
Inhibitory Effect ◽  
Isolation And Identification ◽  
Active Compounds ◽  
Bitter Orange ◽  
Wide Range ◽  
Different Types ◽  
Amoxicillin Clavulanic Acid

The study in duded isolation and identification of microbial isolates from oral cavity to 10 volunteers, diagnosed within the three groups: Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus spp. and Candida albicans . The sensitivity test of all isolates bacteria Streptococcus spp. , S. aureus and S. epidermidis showed high resistance to Ampicillin(100)%,followed Methicillin (88.88)% and Amoxicillin / clavulanic acid(77.77)%, while the resistance for each of Vancomycin and Amoxicillin were (66.66)%, and the resistance to Erythromycin and Pencillin (55.55)% to each of them. The results showed less resistance to Trimethoprim (22.22)% and Cefalotine (11.11)% of all bacteria isolate. Investigation of the presence of active compounds in each of the hot and cold (water and alcoholic) extracts flowers Rose and flowers blossom bitter orange ( new preparatory 2012) (Alkaloids, Quartet alkaloids, sugars, Saponine, Flavones and comarins ) was carried out includes. While it was all kinds of extracts does not contain resins. The results showed the presence of active compounds (Sugars, Flavones and comarins) in old extracts that preparation after a year (2011). PH values of the plant extracts hot and cold (water and alcoholic) for each of orange flowers blossom and Rose flowers (Old and new preparatory) with a wide range ranging between (3.6 -6.4). All extracts hot and cold (alcoholic) (new preparatory 2012) showed most effective towards Streptococcus spp., S. epidermidis and Candida albicans, while the extracts show weak effective against S. aureus. Hot alcohol extracts of Rose (old preparatory 2011) showed most effective towards S. aureus and S. epidermidis only, while the other extracts of old perpetration show weak effective against Streptococcus spp. and C. albicans.

scholarly journals IDENTIFICATION AND ANALYSIS OF ACTIVE CAROTENOID COMPOUNDS FROM ENTAWAK FRUIT (ARTOCARPUS ANISOPHYLLUS)

2021 ◽  
Vol 8 (2) ◽  
pp. 136
Author(s):  
Gervacia Jenny Ratnawaty ◽  
Ratih Indrawati ◽  
Jajar Pramata Syari
Keyword(s):  
Extraction Process ◽  
Carotenoid Content ◽  
Carotenoid Pigments ◽  
Test Results ◽  
Total Carotenoid ◽  
Phytochemical Screening ◽  
Isolation And Identification ◽  
Active Compounds ◽  
Total Carotenoid Content ◽  
Chromatographic Profile

— Isolation and identification of active compounds from mentawak fruit (Artocarpus Anisophyllus) used a plant determination test to determine the plant content. Furthermore, the extraction process was carried out to obtain a crude extract of carotenoid pigments by maceration method using n-hexane as solvent. Furthermore, the obtained macerate was carried out by phytochemical tests to determine the content of the active compounds. The TLC test was carried out to confirm the presence of a positive group of compounds on phytochemical screening, and to determine the chromatographic profile of the extract.  The identification results showed that the phytochemicals showed that the active compounds contained in macerate were alkaloids, flavonoids, tannins and saponins. The KLT test results obtained an Rf value of 0.875. While the total carotenoid content using a spectrophotometer UV-Vis is 958 µg/ml.

scholarly journals Isolation and Identification of Potent Antidiabetic Compounds from Antrodia cinnamomea—An Edible Taiwanese Mushroom

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2864 ◽  
Author(s):  
Hung Huang ◽  
San-Lang Wang ◽  
Van Nguyen ◽  
Yao-Haur Kuo
Keyword(s):  
Type 2 Diabetes ◽  
Natural Resource ◽  
Fruiting Bodies ◽  
Inhibitory Effects ◽  
Meoh Extract ◽  
Isolation And Identification ◽  
Active Compounds ◽  
Antrodia Cinnamomea ◽  
Glucosidase Inhibitors

Antrodia cinnamomea (AC), an edible Taiwanese mushroom, has been recognized as a valuable natural resource with vast biological and medicinal benefits. Recently, the hypoglycemic and anti-diabetic effects of AC were mentioned in several studies. However, no studies have investigated α-glucosidase inhibitors from AC fruiting bodies (ACFB) as they relate to type 2 diabetes (T2D) treatment. The purpose of this study was to gain evidence of potent α-glucosidase inhibitory effects, as well as isolate, identify and characterize the active compounds of ACFB. The MeOH extract of ACFB demonstrated potent α-glucosidase inhibitory activity, and possessed high pH stability (pH 2–11) and thermostable properties at 40–50 °C. Further purification led to the isolation of eight constituents from ACFB, identified as: 25S-antcin K (1), 25R-antcin K (2), dehydrosulphurenic acid (3), 25S-antcin I (4), 25S-antcin B (5), 25R-antcin B (6), dehydroeburicoic acid (7) and eburicoic acid (8). Notably, the ACFB extract and its identified compounds, except 1, 4, and 6 demonstrated a greater effect (EC50 = 0.025–0.21 mg/mL) than acarbose (EC50 = 0.278 mg/mL). As such, these active compounds were determined to be new potent mushroom α-glucosidase inhibitors. These active compounds were also identified on the HPLC fingerprints of ACFB.

Bioassay-guided isolation and identification of anti-platelet-active compounds from the root of Ashitaba (Angelica keiskeiKoidz.)

2014 ◽  
Vol 28 (24) ◽  
pp. 2312-2316 ◽  
Author(s):  
Dong Ju Son ◽  
Ye Oak Park ◽  
Chengguang Yu ◽  
Sung Eun Lee ◽  
Young Hyun Park
Keyword(s):  
Isolation And Identification ◽  
Active Compounds
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