Characterization and genetic mapping of the β-diketone deficient eceriferum-b barley mutant

2017 ◽  
Vol 130 (6) ◽  
pp. 1169-1178 ◽  
Author(s):  
Qin Zhou ◽  
Chao Li ◽  
Kohei Mishina ◽  
Jiecai Zhao ◽  
Jiwei Zhang ◽  
...  
Keyword(s):  
Genetic Mapping ◽  
Barley Mutant

scholarly journals Characterization and genetic mapping of eceriferum-ym (cer-ym), a cutin deficient barley mutant with impaired leaf water retention capacity

2015 ◽  
Vol 65 (4) ◽  
pp. 327-332 ◽  
Author(s):  
Chao Li ◽  
Cheng Liu ◽  
Xiaoying Ma ◽  
Aidong Wang ◽  
Ruijun Duan ◽  
...  
Keyword(s):  
Genetic Mapping ◽  
Water Retention ◽  
Leaf Water ◽  
Water Retention Capacity ◽  
Retention Capacity ◽  
Barley Mutant

Phenotypic Characterization and Genetic Mapping of an Increased Stigma Mutant in Rice (Oryza sativa L.)

2011 ◽  
Vol 37 (10) ◽  
pp. 1779-1784
Author(s):  
Ming-Jing ZHOU ◽  
Yong WEN ◽  
Shuang-Cheng LI ◽  
Cheng-Bo LI ◽  
Man-Hua ZHANG ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Genetic Mapping ◽  
Oryza Sativa L ◽  
Phenotypic Characterization

scholarly journals Characterization and in Vivo Cloning of prlC, a Suppressor of Signal Sequence Mutations in Escherichia coli K12

Genetics ◽  
1987 ◽  
Vol 116 (4) ◽  
pp. 513-521
Author(s):  
Nancy J Trun ◽  
Thomas J Silhavy
Keyword(s):  
Escherichia Coli ◽  
Genetic Mapping ◽  
Signal Sequence ◽  
Illegitimate Recombination ◽  
Mutant Phenotype ◽  
E Coli ◽  
Physical Location ◽  
Sequence Deletion ◽  
New Alleles

ABSTRACT The prlC gene of E. coli was originally identified as an allele, prlC1, which suppresses certain signal sequence mutations in the genes for several exported proteins. We have isolated six new alleles of prlC that also confer this phenotype. These mutations can be placed into three classes based on the degree to which they suppress the lamBsignal sequence deletion, lamBs78. Genetic mapping reveals that the physical location of the mutations in prlC correlates with the strength of the suppression, suggesting that different regions of the gene can be altered to yield a suppressor phenotype. We also describe an in vivo cloning procedure using λplacMu9H. The procedure relies on transposition and illegitimate recombination to generate a specialized transducing phage that carries prlC1. This method should be applicable to any gene for which there is a mutant phenotype.

scholarly journals GENETIC MAPPING OF GENES REQUIRED FOR MOTILITY IN CAULOBACTER CRESCENTUS

Genetics ◽  
1984 ◽  
Vol 108 (3) ◽  
pp. 523-532
Author(s):  
Bert Ely ◽  
Ronda H Croft ◽  
Connie J Gerardot
Keyword(s):  
Genetic Mapping ◽  
Caulobacter Crescentus ◽  
Pleiotropic Effect ◽  
Structure And Function ◽  
Bacteriophage Resistance ◽  
And Function ◽  
Chromosomal Map ◽  
Bacterial Systems

ABSTRACT Mutations in more than 30 genes affect motility in Caulobacter crescentus. We have determined the chromosomal map locations for 27 genes involved in flagellar morphogenesis (fla), three genes involved in flagellar function (mot), and three genes that have a pleiotropic effect on both motility and bacteriophage resistance (ple). Three multigene clusters have been detected at widely separated chromosomal locations, but in addition, there are 12 fla and mot genes that are found at eight additional sites scattered around the C. cresentus chromosome. Thus, there is more scatter of genes involved in flagellar structure and function than has been observed in other bacterial systems.

scholarly journals Fast genetic mapping using insertion-deletion polymorphisms in Caenorhabditis elegans

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ho-Yon Hwang ◽  
Jiou Wang
Keyword(s):  
Caenorhabditis Elegans ◽  
Genetic Mapping ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Genetic Material ◽  
Mapping Method ◽  
Forward Genetics ◽  
Whole Genome ◽  
Nucleotide Polymorphisms ◽  
Large Populations

AbstractGenetic mapping is used in forward genetics to narrow the list of candidate mutations and genes corresponding to the mutant phenotype of interest. Even with modern advances in biology such as efficient identification of candidate mutations by whole-genome sequencing, mapping remains critical in pinpointing the responsible mutation. Here we describe a simple, fast, and affordable mapping toolkit that is particularly suitable for mapping in Caenorhabditis elegans. This mapping method uses insertion-deletion polymorphisms or indels that could be easily detected instead of single nucleotide polymorphisms in commonly used Hawaiian CB4856 mapping strain. The materials and methods were optimized so that mapping could be performed using tiny amount of genetic material without growing many large populations of mutants for DNA purification. We performed mapping of previously known and unknown mutations to show strengths and weaknesses of this method and to present examples of completed mapping. For situations where Hawaiian CB4856 is unsuitable, we provide an annotated list of indels as a basis for fast and easy mapping using other wild isolates. Finally, we provide rationale for using this mapping method over other alternatives as a part of a comprehensive strategy also involving whole-genome sequencing and other methods.

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