Cytotoxicity and genotoxicity of the carcinogen aristolochic acid I (AA-I) in human bladder RT4 cells

2021 ◽  
Author(s):  
Medjda Bellamri ◽  
Kyle Brandt ◽  
Christina V. Brown ◽  
Ming-Tsang Wu ◽  
Robert J. Turesky
Keyword(s):  
Aristolochic Acid ◽  
Human Bladder ◽  
Aristolochic Acid I

Lupane Type Triterpene Isolated from the Leaves of Thecacoris Annobonea (Euphorbiaceae)

2013 ◽  
Vol 12 (10) ◽  
pp. 695-701
Author(s):  
Herve Martial Poumale Poumale ◽  
Alphonsine Nkapwa Guedem ◽  
Louis Pergaud Sandjo ◽  
Bonaventure Tchaleu Ngadjui ◽  
Yoshihito Shiono
Keyword(s):  
Antimicrobial Activity ◽  
Root Growth ◽  
Betulinic Acid ◽  
Aristolochic Acid ◽  
2D Nmr ◽  
Aristolochic Acid I

A new lupane type triterpene (1), together with betulinic acid (2), friedelin (3), aristolochic acid I (4), alpinumisoflavone (5) and 4’-O-methylepinumisoflavone (6) have been isolated from the leaves of Thecacoris annobonea. The structure of the new compound was elucidated on the basis of 1 and 2D NMR experiments. The isolated compounds were evaluated for their phytotoxicity and antimicrobial activity. 1 exhibited significant antimicrobial activity at 30 μg/ml and compounds 1, 2, 3, 4, 5 and 6 inhibited root growth lettuce at 100 μg/ml. 

scholarly journals Solid-Phase Extraction of Aristolochic Acid I from Natural Plant Using Dual Ionic Liquid-Immobilized ZIF-67 as Sorbent

Separations ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 22
Author(s):  
Pei Chen ◽  
Xiaoman Li ◽  
Xuemin Yan ◽  
Minglei Tian
Keyword(s):  
Solid Phase Extraction ◽  
Aristolochic Acid ◽  
Solid Phase ◽  
Phase Extraction ◽  
Liquid Adsorption ◽  
Successful Separation ◽  
Metal Organic ◽  
Herbal Plants ◽  
Aristolochic Acid I ◽  
Herbal Plant

(1) Background: ZIF-67 is one of the most intriguing metal–organic frameworks already applied in liquid adsorption. To increase its adsorption performance, dual ionic liquids were immobilized on ZIF-67 in this research; (2) Methods: The obtained sorbent was used to adsorb aristolochic acid I (AAI) in standard solutions. Then, the sorbent was applied in solid-phase extraction to remove AAI from Fibraurea Recisa Pierre extracted solution. (3) Results: By analyzing the adsorption models, the highest adsorption capacity of immobilized sorbent (50.9 mg/g) was obtained at 25 °C within 120 min. In the SPE process, 0.02 mg of AAI was removed per gram of herbal plant, the adequate recoveries were in the range of 96.2–100.0%, and RSDs were 3.5–4.0%; (4) Conclusions: The provided experimental data revealed that ZIF-67@EIM-MIM was an excellent potential sorbent to adsorb and remove AAI from herbal plant extract, and the successful separation indicated that this sorbent could be an ideal material for the pretreatment of herbal plants containing AAI.

scholarly journals Aristolochic Acid I-Induced Hepatotoxicity in Tianfu Broilers Is Associated with Oxidative-Stress-Mediated Apoptosis and Mitochondrial Damage

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3437
Author(s):  
Dan Xu ◽  
Lizi Yin ◽  
Juchun Lin ◽  
Hualin Fu ◽  
Xi Peng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aristolochic Acid ◽  
Liver Weight ◽  
Mitochondrial Damage ◽  
Chinese Herbs ◽  
Farm Animals ◽  
Mitochondrial Depolarization ◽  
Subchronic Toxicity ◽  
Serum Glutamic Oxalacetic Transaminase ◽  
Aristolochic Acid I

Aristolochic acid (AA) is a component of traditional Chinese herbs and commonly used for farm animals in China. Over-exposure of AA has been proven to be associated with hepatotoxicity; however, the mechanism of action of AA-I-induced hepatotoxicity remains unknown. In the current study, a subchronic toxicity test was conducted to evaluate the mechanism of AA-induced hepatotoxicity in Tianfu broilers. According to the results, AA-I-induced hepatotoxicity in Tianfu broilers was evidenced by the elevation of liver weight, levels of serum glutamic oxalacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT). Furthermore, hepatocyte swelling, vesicular degeneration and steatosis were observed. Additionally, AA-I elevated the production of reactive oxygen species (ROS) and induced oxidative stress, which further led to excessive apoptosis, characterized by mitochondrial depolarization, upregulation of Bax, and down-regulation of Bcl-2 expression. In conclusion, the mechanism of AA-I-induced hepatotoxicity was associated with oxidative-stress-mediated apoptosis and mitochondrial damage.

ACB-PCR measurement of H-ras codon 61 CAA→CTA mutation provides an early indication of aristolochic acid I carcinogenic effect in tumor target tissues

2012 ◽  
Vol 53 (7) ◽  
pp. 495-504 ◽  
Author(s):  
Yiying Wang ◽  
Volker M. Arlt ◽  
Candice A. Roufosse ◽  
Karen L. McKim ◽  
Meagan B. Myers ◽  
...  
Keyword(s):  
Aristolochic Acid ◽  
Tumor Target ◽  
Carcinogenic Effect ◽  
Early Indication ◽  
Aristolochic Acid I ◽  
Codon 61

ISOLATION AND CHARACTERIZATION OF ARISTOLOCHIC ACID - I FROM ARISTOLOCHIA INDICA LINN. LEAVES

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (09) ◽  
pp. 52-53
Author(s):  
M.B Mulik ◽  
◽  
K.S. Laddha
Keyword(s):  
Aristolochic Acid ◽  
Chloroform Extract ◽  
Isolation Method ◽  
Aristolochic Acids ◽  
Isolation And Characterization ◽  
Isolated Compound ◽  
Anti Fungal ◽  
Aristolochic Acid I ◽  
Aristolochia Species

Aristolochic Acids (AAs) are major components of plants in Aristolochia species. The plant Aristolochia indica Linn. has diverse biological actions such as analgesic, anti-diabetics, anti-bacterial, anti-fungal and treatment of malaria and fevers. Here, we report a new and simple isolation method of Aristolochic Acid-Ι (AA-I) from leaves of Aristolochia indica Linn. Defatted leaves of A. indica were extracted with chloroform by soxhlation. AA-I was further isolated from the chloroform extract by partitioning with aqueous sodium bicarbonate. AA-I was purified by repetitive washings with methanol and recrystallization in methanol yielded yellow leaflets. The purity of the isolated compound was ascertained by HPLC analysis. structural elucidation of the isolated compound was done by IR, MS and NMR spectral analysis.

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