ISOLATION AND CHARACTERIZATION OF ARISTOLOCHIC ACID - I FROM ARISTOLOCHIA INDICA LINN. LEAVES

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (09) ◽  
pp. 52-53
Author(s):  
M.B Mulik ◽  
◽  
K.S. Laddha
Keyword(s):  
Aristolochic Acid ◽  
Chloroform Extract ◽  
Isolation Method ◽  
Aristolochic Acids ◽  
Isolation And Characterization ◽  
Isolated Compound ◽  
Anti Fungal ◽  
Aristolochic Acid I ◽  
Aristolochia Species

Aristolochic Acids (AAs) are major components of plants in Aristolochia species. The plant Aristolochia indica Linn. has diverse biological actions such as analgesic, anti-diabetics, anti-bacterial, anti-fungal and treatment of malaria and fevers. Here, we report a new and simple isolation method of Aristolochic Acid-Ι (AA-I) from leaves of Aristolochia indica Linn. Defatted leaves of A. indica were extracted with chloroform by soxhlation. AA-I was further isolated from the chloroform extract by partitioning with aqueous sodium bicarbonate. AA-I was purified by repetitive washings with methanol and recrystallization in methanol yielded yellow leaflets. The purity of the isolated compound was ascertained by HPLC analysis. structural elucidation of the isolated compound was done by IR, MS and NMR spectral analysis.

Isolation and characterization of novel protein with anti-fungal and anti-inflammatory properties from Aloe vera leaf gel

2011 ◽  
Vol 48 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Swagata Das ◽  
Biswajit Mishra ◽  
Kamaldeep Gill ◽  
Md. Saquib Ashraf ◽  
Abhay Kumar Singh ◽  
...  
Keyword(s):  
Aloe Vera ◽  
Isolation And Characterization ◽  
Anti Inflammatory ◽  
Anti Fungal ◽  
Novel Protein

A review on phyto-pharmacology of Oxalis corniculata

2021 ◽  
Vol 24 ◽  
Author(s):  
Iqra Sarfraz ◽  
Azhar Rasul ◽  
Ghulam Hussain ◽  
Muhammad Ajmal Shah ◽  
Bushra Nageen ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Current Effort ◽  
Isolation And Characterization ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Anti Fungal ◽  
Warm Temperate

: Oxalis corniculata (Oxalidaceae) is a small decumbent and delicate appearing medicinal herb flourishing in warm temperate and tropical domains such as Pakistan and India. Main bioactive chemical constituents of Oxalis plant include several alkaloids, flavonoids, terpenoids, cardiac glycosides, saponins, phlobatannins along with steroids. Due to its polyphenolic, glycosides and flavonoid profile, it is proved to be protective in numerous ailments and exhibit various biological activities such as anti-fungal, anti-cancer, anti-oxidant, anti-bacterial, anti-diabetic, and cardioprotective. Moreover, bioactive phytochemicals from this plant possess significant wound healing potential. Our current effort intends to emphasize on the immense significance of this plant species, which have not been the subject matter of clinical trials and effective pharmacological studies, even though its favored usage has been stated. This review proposes that Oxalis corniculata possess potential for the cure of various diseases, however, further researches on isolation and characterization of bioactive compounds along with pre-clinical trials are compulsory to figure out its pharmacological applications.

scholarly journals Isolation and Characterization of Hypolipidemic Compound from Cajanus cajan

2011 ◽  
Vol 24 (1) ◽  
pp. 6-10
Author(s):  
M Anwar Habib ◽  
ASM Anisuzzaman ◽  
RK Barman ◽  
M Abdul Gafur ◽  
M Tofazzal Haque
Keyword(s):  
Cajanus Cajan ◽  
Methanol Extract ◽  
Fasting Blood Glucose ◽  
Hypolipidemic Activity ◽  
1H And 13C Nmr ◽  
Isolation And Characterization ◽  
Nmr Data ◽  
Biological Studies ◽  
Isolated Compound

The study was carried out to identify the compound responsible for hypolipidemic and hypoglycemic effects of Cajanus cajan (redgram). The methanol extract of redgram seeds was found to decrease fasting blood glucose and lipid profile (p<0.001) on streptozotocin-induced mice compared to control. This activity- guided fraction led to the isolation of a compound, substituted benzene containing polyhydroxy functions fused with lactone (CCA3) by analysis of 1H and 13C-NMR data. Biological studies of the isolated compound possessed prominent hypolipidemic activity. Although a number of hypoglycemic compounds are reported, yet not any hypolipidemic compound from redgram. The compound CCA3 seems to be the first report on hypolipidemic activity from methanol extract of redgram. TAJ 2011; 24(1): 6-10

scholarly journals Isolation and Characterization of Antimicrobial Compound from Stem Bark of Traditional Medicinal Plant Sonneratia apetala: Evaluation of their antimicrobial, Cytotoxic and Antioxidant Properties

2021 ◽  
Vol 38 (1) ◽  
pp. 1-5
Author(s):  
Md Arifur Rahman ◽  
Ahmed Abu Rus’d ◽  
Md Enamul Haque
Keyword(s):  
Brine Shrimp ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
Chloroform Extract ◽  
Free Radical Scavenging ◽  
Gram Negative Bacteria ◽  
Isolation And Characterization

Sonneratia apetala (S. apetala) (Lythraceae) has been investigated for the isolation and characterization of antimicrobial compounds and evaluation of their biological activities. The chloroform extract of the stem bark and different partitionate of the chloroform extracts i.e. Petroleum ether soluble fraction (PESF), Ethyl acetate soluble fraction(EASF), Methanol soluble fraction(MSF) and aqueous soluble fractions (ASF) were subjected to different chromatographic techniques to isolate secondary metabolites. Successive chromatographic separation and purification yielded a total of two compounds identified and characterized as Taraxerone(1) and 5,8-dihydroxy- 6-methoxy-4,9-dioxo-1,3,4,9-tetrahydronaphthol[2,3-c]furan-1-yl acetate (2) by extensive proton NMR spectrum (1H-NMRspectrum) analysis. The different partitionate like PESF, EASF, MESF and ASF were subjected to screen their antimicrobial properties against some selected Gram positive and Gram negative bacteria and fungi, brine shrimp lethality and antioxidant activities. The maximum zone of inhibition of chloroform extract was found against Pseudomonas sp. (16mm). All fractions showed more activity against Gram negative bacteria then Gram positive bacteria. In the brine shrimp lethality bioassay, among all extracts, the petroleum ether and ethyl acetate soluble fraction showed significant lethality having the LC50 value of 7.72 μg/ml. The antioxidant activity was evaluated in terms of determination of free radical scavenging activity (DPPH assay). Among all the extracts of S. apetala the highest free radical scavenging activity showed by (Methanol soluble fraction) MESF with IC50 value 18.0 μg/ml. Bangladesh J Microbiol, Volume 38, Number 1, June 2021, pp 1-5

scholarly journals QuEChERS pretreatment combined with high-performance liquid chromatography-tandem mass spectrometry for determination of aristolochic acids I and II in Chinese herbal patent medicines

RSC Advances ◽  
2020 ◽  
Vol 10 (42) ◽  
pp. 25319-25324
Author(s):  
Jinghe Zhang ◽  
Yinan Wang ◽  
Jing Sun ◽  
Guowei Zhou ◽  
Xiaojie Jiang ◽  
...  
Keyword(s):  
High Performance ◽  
Aristolochic Acid ◽  
Patent Medicines ◽  
Chinese Herbal ◽  
Aristolochic Acids ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Aristolochic Acid I ◽  
Carcinogenic Compound

Aristolochic acid I and II (AA I and II), a kind of nephrotoxic and carcinogenic compound, are widely added in Chinese herbal patent medicines though they have been banned due to their toxicity.

scholarly journals Isolation, characterization and biological properties of betulin from Entada africana Guill. and Perr. (Mimosaceae).

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
A. Kwaji ◽  
H. M. Adamu ◽  
I. Y. Chindo ◽  
R. Atiko
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Biological Properties ◽  
Pure Compound ◽  
Isolation And Characterization ◽  
Isolated Compound

The present study is aimed at the isolation and characterization of betulin from Entada africana.  A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.

scholarly journals Isolation and Characterisation of Hydrolysable Tannin from Ethyl Acetate Portion of the Aerial Part of Phyllanthus amarus Schum. & Thonn

2019 ◽  
pp. 1-10
Author(s):  
Halima A. Umar ◽  
Hamidu Usman ◽  
Mustapha B. Abubakar ◽  
Baba F. Mohammed ◽  
Mohammed Babakura ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Aerial Part ◽  
Methanol Extract ◽  
Phyllanthus Amarus ◽  
Hydrolysable Tannin ◽  
Isolation And Characterization ◽  
Hydrolysable Tannins ◽  
Isolated Compound ◽  
Layer Chromatography

The present work involves extraction of phytochemicals from aerial part of Phyllanthus amarus Schum. & Thonn with n-hexane and 85% methanol. The isolation and characterization of Phytoconstituents was done from the methanol extract through portioning with chloroform and ethyl acetate. Fractionation and isolation (using column and thin layer chromatography respectively) of ethyl acetate column pooled portion afforded a compound coded as E-3.3C. The structure of the isolated compound was established on spectroscopic evidences (IR, 1HNMR, MS), which revealed the compound as 1-de (oxygalloyl)-2ˈ,3ˈ,-di-methoxy-amariin a hydrolysable tannins.

scholarly journals Isolation and Characterization of Antitumor Alkaloid from Poppy Capsules (Papaver somniferum)

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Ibrahim Bulduk ◽  
Fulya Taktak
Keyword(s):  
Papaver Somniferum ◽  
Toxicity Profile ◽  
Isolation And Characterization ◽  
Cancer Types ◽  
Ft Ir ◽  
Low Toxicity ◽  
Increasing Demand ◽  
Isolated Compound ◽  
First Time

Noscapine, a phthalideisoquinoline alkaloid derived from opium, has been used in the treatment of various cancer types. Its low-toxicity profile has increased attention to this alkaloid. With regard to increasing demand for this compound, we developed a new method for isolation of noscapine from dried capsules ofPapaver somniferum. Noscapine was successfully isolated from poppy capsules for the first time and the purity of the isolated compound was determined to be over 99.59% by HPLC analysis. The structure of noscapine was confirmed by NMR, NMR, FT-IR, elemental analysis, and HR-ESI-MS methods.

Isolation and Characterization of Vanillin from Coconut Husk Lignin via Alkaline Nitrobenzene Oxidation

2014 ◽  
Vol 67 (4) ◽  
Author(s):  
Norzita Ngadi ◽  
Noor Amirah Abdul Halim ◽  
Mohammad Nasir Mohamad Ibrahim
Keyword(s):  
Gas Chromatography ◽  
Infrared Spectroscopy ◽  
Crystallization Process ◽  
Black Liquor ◽  
Coconut Husk ◽  
Isolation And Characterization ◽  
Ftir Characterization ◽  
Isolated Compound ◽  
Detection And Quantification

Isolation of vanillin from lignin extracted from coconut husk is presented in this study. Phosphoric acid (20%) is employed to precipitate the lignin from the black liquor produced by cooking the mixture of coconut husk and 1M NaOH in steam autoclave at 121ºC for one hour. Separation of vanillin from lignin was carried out via oxidation by adding 0.1 g of coconut husk lignin into a mixture of 2M NaOH (10 ml) and nitrobenzene (0.4 ml) in steel capillary bomb heated at 160ºC for 2.5 hours. Detection and quantification of vanillin presents in the lignin oxidized mixture was performed by liquid and gas chromatography. The recovery of vanillin from the mixture was done via crystallization process by dissolving the mixture in acetone and heats up to 60ºC with periodic stirring. Vanillin crystal appeared by rapidly cooled the heated mixture in the ice bath. The vanillin obtained was characterized by Infrared Spectroscopy (FTIR) for component verification. Component isolation by HPLC and GC-FID denoted the retention time of vanillin appeared around 18.852 minutes and 16.913 minutes respectively. A yield of 2.83 % vanillin was isolated from 0.1 g lignin sample and the result of FTIR characterization has suggested that the isolated compound is vanillin.

scholarly journals Rapid Analysis of Aristolochic Acid Analogues in Traditional Chinese Patent Medicine by LC-MS/MS

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Jing Liu ◽  
Yang Liu ◽  
Yingxue Wu ◽  
Zhong Dai ◽  
Shuangcheng Ma
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Aristolochic Acid ◽  
Gene Mutations ◽  
Aristolochic Acids ◽  
Potential Safety ◽  
Akebiae Caulis ◽  
The One ◽  
Traditional Chinese Patent Medicine ◽  
Aristolochic Acid I

Aristolochic acids have been demonstrated to have renal toxicity, cause carcinogenesis, and may cause gene mutations. A series of risk control measurements have been adopted worldwide since 1990s. Some varieties of traditional Chinese medicine with high content of aristolochic acids have been banned in China. However, some species containing aristolochic acids in microscale are still in use. In recent years, with the continuous awareness of drug safety, the aristolochic acid analogues were generally considered to be of potential safety risks. Among these constituents, aristolochic acid I is still the one with most studies. Therefore, in addition to aristolochic acid I, it is necessary to establish an accurate and rapid method to determine other aristolochic acid analogues. LC-MS/MS methods based on multireaction monitoring mode was established to simultaneously determine 9 aristolochic acid analogues including 5 aristolochic acids and 4 aristolactams for the first time. Furthermore, the method was applied for Long dan Xie gan Pill, a traditional complex compound preparation with a long history for treatment of diseases including hepatochlic hygropyrexia, dizziness, tinnitus, and deafness. It has attracted widespread attention because of the aristolochic acid nephropathy. The crude drug Caulis Aristolochiae manshuriensis (Guanmutong) collected in the prescription was replaced by Akebiae Caulis (Mutong), and the established method helps to understand the product safety on market. As a result, aristolochic acid I, aristolochic acid Iva, and aristolactam I were detected and determined in one batch of Long dan Xie gan Pill among 25 batches of samples. It provided practical approach to demonstrate trace aristolochic acids and aristolactams. It was beneficial to control the safety of related traditional Chinese medicine products.

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