Quantification of hydroxycinnamic derivatives in wines by UHPLC-MRM-MS

Chañar (Geoffroea decorticans- Fabaceae) is a tree from South America that is normally infected with galls originated by insects. One of its parasites is Allodiplosis crassa (Cecidomyiidae, Diptera) which produces globular galls with sticky prolongations. Since this plant has medicinal uses in Argentina, its infestation could alter the quality of the plant drug. The surface of insect-induced galls usually contains defensive features such as trichomes, increased hardness and an increase in the content of polyphenolic compounds. The objective of this research is to assess the structural and histochemical features of the gall and to compare the content of polyphenolic metabolites in the gall, in the healthy leaf and in lignified stems of G. decorticans. The methanolic extract from the galls showed the highest amount of polyphenolic and proanthocyanidins and the lowest amount of hydroxycinnamic derivatives and flavonoids compared to the methanolic extract of the leaves. The photographs taken from the external surface of the gall showed that some prolongations have heads. The histochemical analysis showed that the prolongations have a high amount of proanthocyanidins and flavonoids; and that the heads are reactive to Sudan III. These phytochemical and histological characteristics may have a defensive role against harmful fungi and parasites that attack the larvae of the A. crassa. The results of this study show the presence of defensive features in an insect-induced gall of a medicinal plant with potential implications in the pharmacological activity of this species. This is the first report of a histochemical and phytochemical study in G. corticans galls.

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Phenolic compounds variation in Mentha L. Species in the course of a four-years period / Kolísanie fenolových látok v rôznych druhoch Mentha L. Počas 4 ročného obdobia

Acta Facultatis Pharmaceuticae Universitatis Comenianae ◽

10.1515/afpuc-2015-0013 ◽

2015 ◽

Vol 62 (s9) ◽

pp. 2-7

Author(s):

S. Fialová ◽

D. Tekeľová ◽

K. Rendeková ◽

J. Klinčok ◽

M. Kolárik ◽

...

Keyword(s):

Phenolic Compounds ◽

Essential Oil ◽

Phenolic Acids ◽

Rosmarinic Acid ◽

Pharmacological Properties ◽

European Pharmacopoeia ◽

Total Polyphenols ◽

Spectrophotometric Methods ◽

Hydroxycinnamic Derivatives

AbstractMints rank among the most important Lamiaceae plants. In addition to essential oil, they contain many valuable phenolic compounds, including flavonoids and phenolic acids that participate in mints’ pharmacological properties. In this work, we examined the contents of phenolic compounds variation in the course of 4 years of vegetation. We compared the contents of total hydroxycinnamic derivatives expressed as rosmarinic acid (λ = 505 nm), total polyphenols and tannins expressed as rosmarinic acid (λ = 760 nm), and flavonoids expressed as luteolin-7-O-glucoside (λ = 392 nm) and quercetin (λ = 420 nm) in 1-, 2-, 3-, and 4-year-old plants’ dry leaves, respectively. Spectrophotometric methods of the European Pharmacopoeia were employed. Our results show high levels of active phenolic compounds, particularly in 3- and 4-year-old plants.

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HPLC-ESI-MS/MS Profiling of Phenolic Acids, Flavonoids And Sesquiterpene Lactones from Xanthium spinosum

Revista de Chimie ◽

10.37358/rc.20.3.8031 ◽

2001 ◽

Vol 71 (3) ◽

pp. 558-564

Author(s):

Erzsebet Varga ◽

Erzsebet Domokos ◽

Hajnal Kelemen ◽

Ibolya Fulop ◽

Laszlo Kursinszki

Keyword(s):

High Performance ◽

Chemical Constituents ◽

Sesquiterpene Lactones ◽

Diverse Range ◽

Caffeoylquinic Acid ◽

Prostate Diseases ◽

First Time ◽

Hydroxycinnamic Derivatives ◽

Urinary Problems

Bathurst burr (Xanthium spinosum L.) is used worldwide in traditional medicine to treat a diverse range of health problems including urinary problems associated with various prostate diseases. The aim of this study was to complete the identification and structural characterization of the chemical constituents from the aerial part of X. spinosum by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry. From the unequivocally detected and characterized compounds of aqueous-methanol extract, protocatechuic acid, 4-O-caffeoylquinic acid and 3,5-di-O-caffeoylquinic acid were found for the first time in X. spinosum. Besides these compounds, 25 phenolics (including 7 hydroxybenzoic derivatives, 13 hydroxycinnamic derivatives, one benzyl alcohol-hexose-pentose, and 4 flavonoids), 6 sesquiterpenes and 3 diterpenes were tentatively identified.

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Quality Control and Analytical Test Method for Taxus baccata Tincture Preparation

Natural Product Communications ◽

10.1177/1934578x1200700717 ◽

2012 ◽

Vol 7 (7) ◽

pp. 1934578X1200700

Author(s):

Pamela Vignolini ◽

Beatrice Gehrmann ◽

Matthias Friedrich Melzig ◽

Leonardo Borsacchi ◽

Arianna Scardigli ◽

...

Keyword(s):

Test Method ◽

Drug Preparation ◽

Taxus Baccata ◽

Quick Response ◽

Polyphenol Content ◽

Total Polyphenol ◽

Analytical Test ◽

Identification Test ◽

Reproducible Method ◽

Hydroxycinnamic Derivatives

The homeopathic tincture of Taxus baccata L. is monographed in the current German Homeopathic Pharmacopoeia (HAB 2009). However, the described identification test is a common comparative TLC procedure that might be updated. The purpose of the current work was the quali-quantitative analysis by HPLC/DAD/MS of Taxus tincture. In this study we characterized polyphenolic compounds, in particular four hydroxycinnamic derivatives (0.85 mg/mL) and four flavonoids (quercetin and kaempferol 3- O-rutinoside and xylosyl glucosides); the total polyphenol content was 1.265 mg/mL of tincture. Starting from the official German Homeopathic Pharmacopoeia method of preparation, the aim of this work was to optimize a rapid and reproducible method for the analysis of herbal drugs and tincture, directly prepared in store or the herbalist's shop, to ensure safety and efficacy of the preparation. The procedure has to ensure validation, robustness of the results, and provide a quick response about the composition of compounds in the herbal drug preparation.

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Cytotoxic and COX-2 Inhibition Properties of Hydroxycinnamic Derivatives

Letters in Drug Design & Discovery ◽

10.2174/157018006777574212 ◽

2006 ◽

Vol 3 (5) ◽

pp. 316-320 ◽

Cited By ~ 10

Author(s):

M. Paula M. Marques ◽

Fernanda Borges ◽

Joana Sousa ◽

Rita Calheiros ◽

Jorge Garrido ◽

...

Keyword(s):

Cox 2 ◽

Hydroxycinnamic Derivatives

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Quantification of some secondary metabolites in selected Cornaceae species / Stanovenie vybraných sekundárnych metabolitov v niektorých druhoch čeľade Cornaceae

Acta Facultatis Pharmaceuticae Universitatis Comenianae ◽

10.1515/afpuc-2015-0008 ◽

2015 ◽

Vol 62 (s9) ◽

pp. 8-11 ◽

Cited By ~ 2

Author(s):

V. Forman ◽

M. Haladová ◽

D. Grančai

Keyword(s):

Secondary Metabolites ◽

Caffeic Acid ◽

Rosmarinic Acid ◽

Plant Material ◽

European Pharmacopoeia ◽

Total Polyphenols ◽

Spectrophotometric Methods ◽

Cornus Mas ◽

Cornus Kousa ◽

Hydroxycinnamic Derivatives

Abstract Leaves of selected species of the Cornaceae family (Swida alba, Swida australis, Swida controversa, Swida coreana, Swida baileyi, Cornus kousa, Cornus mas) were investigated in this study. These shrubs or small trees with hard wood are cultivated in Slovakia (Tesarske Mlyňany Arboretum). Some species (C. mas and C. kousa) have edible fruits. Cornels find broad ethnopharmacological use, for example, in Traditional Chinese Medicine (TCM), where fruits of C. officinalis are used for the treatment of several disorders, such as organ hypersecretion or diabetes. They are also used as tonics and for astringent effects. Leaves and fruits of C. kousa have been reported to possess antioxidative, anti-inflammatory and antidiabetic properties. The aim of our study is to compare the content of selected secondary metabolites groups in the leaves of above-mentioned species. The plant material was collected in the Tesarske Mlyňany Arboretum, Slovakia, in September 2010. Modified spectrophotometric methods of the European Pharmacopoeia, 8th edition (Ph. Eur. 8) were used for all assays. Results show that the total hydroxycinnamic derivatives (THD) content varied from 1.14 (S. alba) to 2.86% (S. coreana) when expressed as rosmarinic acid, and from 1.01 (S. alba) to 2.55% (S. coreana) when expressed as caffeic acid. The flavonoids content ranged from 0.21 (C. mas) to 1.37% (S. coreana), total polyphenols content from 9.6 (C. kousa) to 7.27% (S. coreana), and tannins content from 7.17 (C. kousa) to 4.04% (S. coreana). Leaves of Korean dogwood (S. coreana) were observed to show the highest content of THD expressed as rosmarinic acid (2.86%) and caffeic acid (2.55%), as well as of flavonoids (1.37%). Kousa dogwood (C. kousa) contained the highest amounts of tannins (7.17%) and total polyphenols (9.6%) on the other hand.

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