HPLC-ESI-MS/MS Profiling of Phenolic Acids, Flavonoids And Sesquiterpene Lactones from Xanthium spinosum
Bathurst burr (Xanthium spinosum L.) is used worldwide in traditional medicine to treat a diverse range of health problems including urinary problems associated with various prostate diseases. The aim of this study was to complete the identification and structural characterization of the chemical constituents from the aerial part of X. spinosum by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry. From the unequivocally detected and characterized compounds of aqueous-methanol extract, protocatechuic acid, 4-O-caffeoylquinic acid and 3,5-di-O-caffeoylquinic acid were found for the first time in X. spinosum. Besides these compounds, 25 phenolics (including 7 hydroxybenzoic derivatives, 13 hydroxycinnamic derivatives, one benzyl alcohol-hexose-pentose, and 4 flavonoids), 6 sesquiterpenes and 3 diterpenes were tentatively identified.