scholarly journals Simultaneous determination of 137 drugs of abuse, new psychoactive substances, and novel synthetic opioids in meconium by UHPLC-QTOF

2021 ◽  
Author(s):  
Ángela López-Rabuñal ◽  
Daniele Di Corcia ◽  
Eleonora Amante ◽  
Marta Massano ◽  
Angelines Cruz-Landeira ◽  
...  
Keyword(s):  
Drugs Of Abuse ◽  
Limit Of Detection ◽  
New Psychoactive Substances ◽  
Range Limit ◽  
Psychoactive Substances ◽  
Childbearing Age ◽  
Two Samples ◽  
Novel Synthetic Opioids ◽  
Substances Of Abuse

AbstractNew psychoactive substances (NPS) have been introduced into the market in recent years, with new analytes reported every year. The use of these substances in women can occur at any stage of life, even in the childbearing age. Drug use during pregnancy presents significant risks for the mother and the fetus, so it is important to have tools that allow to detect prenatal exposure to these substances of abuse. Therefore, an analytical method for the determination of 137 NPS and other drugs of abuse in meconium by UHPLC-QTOF was developed and validated for semi-quantitative purpose. Linearity range, limit of detection (LOD), precision, matrix effect, selectivity, and specificity were evaluated. For all analytes, the calibration curves were studied in the ranges between 2, 10, or 50 ng/g and 750 or 1000 ng/g, (depending on the analyte) and the LOD ranged between 0.04 and 2.4 ng/g. The method was applied to 30 meconium specimens from cases in which fentanyl had been administered as epidural anesthesia at the time of delivery or cases in which the maternal hair was positive to other drug of abuse. Four meconium samples tested positive for fentanyl (range concentration = 440–750 ng/g) and two samples tested positive to acetylfentanyl (range concentration = 190–1400 ng/g).

scholarly journals DEVELOPMENT AND APPLICATION OF A RAPID SCREENING METHOD FOR DETERMINATION OF NEW PSYCHOACTIVE SUBSTANCES AND THEIR METABOLITES IN URINE

2019 ◽  
Vol 16 (33) ◽  
pp. 206-224
Author(s):  
K. M. SHESTAKOVA ◽  
S. A. SAVCHUK ◽  
N. V. MESONZHNIK ◽  
A. V. KUHARENKO ◽  
S. A. APPOLONOVA
Keyword(s):  
Mass Spectrometry ◽  
Limit Of Detection ◽  
Screening Method ◽  
Mass Spectrometry Analysis ◽  
Rapid Screening ◽  
New Psychoactive Substances ◽  
Preparation Technique ◽  
Psychoactive Substances ◽  
Urine Specimens

The ongoing appearance of new psychoactive substances on the black market of illegal drugs, as well as the lack of information on their influence on the human body, faces several challenges in their determination by standard analytical techniques. Moreover, the rapid metabolism of new psychoactive substances reveals in the absence of possibility in the identification of their native structures in biological fluids. This study presents a new screening method for determination 137 psychoactive substances including their metabolites. 'Dilute-and-shoot' method was chosen as the preferable sample preparation technique, and consisted of 1:5 dilution of urine specimens with the solution of acetonitrile and water (30:70) followed by electrospray ionization – liquid chromatography-tandem mass spectrometry analysis. The developed qualitative method was validated according to United Nations Office on Drugs and Crime requirements that included assessment of selectivity, limits of detection, precision, and stability. In addition, the presented method was tested on 50 certified positive urine specimens containing different drugs of abuse. The confirmatory analysis was performed using a high-resolution mass-spectrometry approach. The presented screening method provides the possibility of simultaneous determination of synthetic cannabinoids (96), opioid analgesics (16), stimulators (13), hallucinogens (5), benzodiazepines (5) and non-classified drugs (10) during one run. The validation assessments of the novel method have shown high rates of its specificity, selectivity, intra- and inter-day precision and stability with the limit of detection ranged from 1 to 5 ng?mL-1. At the same time, tests of 50 positive samples showed excellent applicability of the developed screening method for routine preliminary screening analysis in toxicological laboratories.

scholarly journals Preparation of Cerium Oxide-MWCNTs Nanocomposite Bulk Modified Carbon Ceramic Electrode: A Sensitive Sensor for Tamoxifen Determination in Human Serum Samples

2021 ◽  
Author(s):  
Sepideh Shafaei ◽  
Elyas Hosseinzadeh ◽  
Gulsah Saydan Kanberoglu ◽  
Balal Khalilzadeh ◽  
Rahim Mohammad-Rezaei
Keyword(s):  
Cerium Oxide ◽  
Limit Of Detection ◽  
Low Cost ◽  
High Electron ◽  
Range Limit ◽  
Serum Samples ◽  
Important Species ◽  
Carbon Ceramic Electrode ◽  
Carbon Ceramic

Abstract In this study, cerium oxide and multi-walled carbon nanotubes nanocomposite was incorporated into the carbon ceramic electrode (CeO2-MWCNTs/CCE) as a renewable electrode for the electrocatalytic purposes. To demonstrate capability of the fabricated electrode, determination of Tamoxifen as an important anticancer drug with differential pulse voltammetry technique was evaluated. Linear range, limit of detection and sensitivity of the developed sensor were found to be 0.2-40 nM, 0.132 nM and 1.478 µA nM-1 cm-2, respectively. Ease of production, low cost and high electron transfer rate of CeO2-MWCNTs/CCE promise it as a novel electro-analytical tool for determination of important species in real samples.

Screening of 104 New Psychoactive Substances (NPS) and Other Drugs of Abuse in Oral Fluid by LC–MS-MS

2020 ◽  
Vol 44 (7) ◽  
pp. 697-707
Author(s):  
Kelly Francisco da Cunha ◽  
Karina Diniz Oliveira ◽  
Marilyn A Huestis ◽  
Jose Luiz Costa
Keyword(s):  
Oral Fluid ◽  
Drugs Of Abuse ◽  
Matrix Effects ◽  
Synthetic Cannabinoids ◽  
Screening Method ◽  
Simultaneous Detection ◽  
New Psychoactive Substances ◽  
Major Public Health Problem ◽  
Psychoactive Substances ◽  
Wide Range

Abstract New psychoactive substances (NPS) are a major public health problem, primarily due to the increased number of acute poisoning cases. Detection of these substances is a challenge. The aim of this research was to develop and validate a sensitive screening method for 104 drugs of abuse, including synthetic cannabinoids, synthetic cathinones, fentanyl analogues, phenethylamines and other abused psychoactive compounds (i.e., THC, MDMA, LSD and their metabolites) in oral fluid by liquid chromatography–tandem mass spectrometry (LC–MS-MS). The Quantisal™ oral fluid device was used to collect oral fluid samples. The oral fluid–elution buffer mixture (500-μL sample) was extracted with t-butyl methyl ether, and chromatographic separation was performed on a Raptor™ biphenyl column (100 × 2.1 mm ID, 2.7 μm), with a total run time of 13.5 min. Limits of detection were established at three concentrations (0.05, 0.1 or 1 ng/mL) for most analytes, except for acetyl norfentanyl and mescaline (5 ng/mL). Matrix effects were generally <20% and overall extraction recoveries >60%. The highest matrix effect was observed within the synthetic cannabinoid group (PB22, −55.5%). Lower recoveries were observed for 2C-T (47.2%) and JWH-175 (58.7%). Recoveries from the Quantisal™ device were also evaluated for all analytes (56.7–127%), with lower recoveries noted for 25I-NBOMe, valerylfentanyl and mCPP (56.7, 63.0 and 69.9%, respectively). Drug stability in oral fluid was evaluated at 15, 60 and 90 days and at 25, 4 and −20°C. As expected, greater stability was observed when samples were stored at −20°C, but even when frozen, some NPS (e.g., synthetic cannabinoids) showed more than 20% degradation. The method was successfully applied to the analysis of seven authentic oral fluid samples positive for 17 different analytes. The method achieved good sensitivity and simultaneous detection of a wide range of NPS.

Recent bionalytical methods for the determination of new psychoactive substances in biological specimens

Bioanalysis ◽  
2020 ◽  
Vol 12 (21) ◽  
pp. 1557-1595
Author(s):  
Ana Y Simão ◽  
Mónica Antunes ◽  
Hernâni Marques ◽  
Tiago Rosado ◽  
Sofia Soares ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Analytical Methods ◽  
Synthetic Cannabinoids ◽  
New Psychoactive Substances ◽  
Screening Methods ◽  
Psychoactive Substances ◽  
Clinical Action ◽  
One Step ◽  
Preparation Techniques

One of the problems associated with the consumption of new psychoactive substances is that in most scenarios of acute toxicity the possibility of quick clinical action may be impaired because many screening methods are not responsive to them, and laboratories are not able to keep pace with the appearance of new substances. For these reasons, developing and validating new analytical methods is mandatory in order to efficiently face those problems, allowing laboratories to be one step ahead. The goal of this work is to perform a critical review regarding bionalytical methods that can be used for the determination of new psychoactive substances (phenylethylamines, cathinones, synthetic cannabinoids, opioids, benzodiazepines, etc), particularly concerning sample preparation techniques and associated analytical methods.

scholarly journals Comparison of six derivatizing agents for the determination of nine synthetic cathinones using gas chromatography-mass spectrometry

2017 ◽  
Vol 9 (18) ◽  
pp. 2732-2743 ◽  
Author(s):  
Khalid A. Alsenedi ◽  
Calum Morrison
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Synthetic Cathinones ◽  
Gas Chromatography Mass Spectrometry ◽  
New Psychoactive Substances ◽  
Psychoactive Substances ◽  
Chromatography Mass Spectrometry ◽  
Synthetic Cathinone ◽  
Insight Into

Insight into mass spectrometry ions and derivatization conditions for synthetic cathinone type new psychoactive substances.

scholarly journals Synthesis and Voltammetric Determination of Pb(II) Using a ZIF-8-Based Electrode

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Dinh Quang Khieu ◽  
Mai Thi Thanh ◽  
Tran Vinh Thien ◽  
Nguyen Hai Phong ◽  
Duc Hoang Van ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Hydrothermal Process ◽  
Specific Area ◽  
Differential Pulse ◽  
Range Limit ◽  
Solution Ph ◽  
Transmission Electron ◽  
Potential Electrode ◽  
Limit Of Quantitation

Zeolite imidazole framework-8 (ZIF-8) was prepared by the hydrothermal process. The obtained ZIF-8 was a characteristic of X-ray-diffraction (XRD), transmission electron microscope (TEM), thermal gravity-differential thermal analysis (TG-DTA), and dynamic light scattering (DLS). The obtained ZIF-8 possessed large specific area and was highly dispersed. Its morphology consisted of nanospherical particles with 30–50 nm in diameter. Chemical stability of ZIF-8 in different conditions was studied. The ZIF-8 was used as an electrode modifier for the determination of trace levels of lead. The parameters including solvents and solution pH were investigated. The repeatability, reproducibility, accuracy, linear range, limit of detection, and limit of quantitation were also addressed. The results showed that ZIF-8 is a potential electrode modifier for differential pulse anodic stripping method to determine Pb(II) in aqueous solution.

scholarly journals Hair Testing for Drugs of Abuse and New Psychoactive Substances in a High-Risk Population

2017 ◽  
Vol 41 (5) ◽  
pp. 376-381 ◽  
Author(s):  
Alberto Salomone ◽  
Joseph J. Palamar ◽  
Enrico Gerace ◽  
Daniele Di Corcia ◽  
Marco Vincenti
Keyword(s):  
High Risk ◽  
Drugs Of Abuse ◽  
New Psychoactive Substances ◽  
High Risk Population ◽  
Psychoactive Substances ◽  
Risk Population ◽  
Hair Testing
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